Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves

Abstract

An ionic liquid aqueous solvent-based microwave-assisted hydrolysis (ILAS-MAH) approach was proposed for the rapid extraction and accurate determination of myricetin and quercetin from Myrica rubra (M. rubra) leaves for the first time. The effects of the ionic liquid class and concentration, liquid-solid ratio, hydrolysis temperature and time were investigated to obtain the optimal ILAS-MAH conditions. The optimized conditions were 2.0 mol/L [bmin][HSO4] or 2.5 mol/L acidified [bmin]Br solution, liquid-solid ratio 30:1 (mL : g), hydrolysis temperature 70°C and hydrolysis time 10 min. Under these conditions, the recoveries of myricetin and quercetin were in the range of 86.3-107.3% with relative standard deviation lower than 5.8%. Compared with conventional heating hydrolysis and regular MAH, the proposed approach reduced hydrolysis time and improved yields. The mechanism of ILAS-MAH was also investigated.

Ionic Liquid-Based Microwave-Assisted Extraction of Flavonoids from Bauhinia championii (Benth.) Benth.

“…IL was only used as a solvent and additive to enhance the hydrolysis of flavonol glycosides. HCl concentration played a very important role in the hydrolysis of flavonol glycosides [ 32 ], so the presence of HCl in [bmim] Br was necessary for the hydrolysis of the myricetin, quercetin and kaempferol glycosides. According to the results of Wang et al .…”

Optimization of ionic liquid based ultrasonic assisted extraction of puerarin from Radix Puerariae Lobatae by response surface methodology

“…These intrinsic features of ILs, combined with their ease of preparation, have resulted in a remarkable increase in their use in academia and industry (Sun & Armstrong, 2010). Now ILs have been successfully applied for the IL based extraction of bioactive compounds (Poole & Poole, 2010) from medicinal plants, such as alkaloids (Cao, Ye, Lu, Yu, & Mo, 2009;Du, Xiao, Xu, & Li, 2010;Lu, Ma, Hu, Dai, & Pan, 2008;Ma et al, 2010;Zhang et al, 2009), lactones (Chi et al, 2011), polyphenolic compounds (Du, Xiao, Luo, & Li, 2009;Du et al, 2007;Ma, Liu, et al, 2011;Ma, Qiao, et al, 2011), flavonoids (Du, Xiao, & Li, 2011;Huang & Xie, 2010;Zeng, Wang, Kong, Nie, & Yuan, 2010), phenolic acids and quinones (Wu et al, 2009). Recently, a novel ionic liquid based ultrasonic assisted extraction (ILUAE) has been developed, combining the advantages of UAE and beneficial properties of ILs.…”

Effect of amino acid-functionalized multi-walled carbon nanotubes on the properties of dopamine-based poly(amide-imide) composites: An experimental study

“…Some of the reported methods require a prolonged reaction time and the use of toxic solvents. The growing interest during the past two decades in the environmental protection has made both modern, academic and industrial groups develop green chemical processes with maximum yield and minimum cost (Du et al 2011;Sugamoto et al 2011). As part of our programme, which aimed at development of synthetic methodologies (Mallakpour and Rafiee 2011) by eliminating flammable, toxic or carcinogenic organic solvents, we employed a molten ionic salt, TBAB, as a green chemical medium for step-growth polymerization.…”