Ionic‐liquid‐based ultrasound‐assisted extraction of isoflavones from Belamcanda chinensis and subsequent screening and isolation of potential α‐glucosidase inhibitors by ultrafiltration and semipreparative high‐performance liquid chromatography

Abstract

The separation of a compound of interest from its structurally similar homologues to produce high-purity natural products is a challenging problem. This work proposes a novel method for the separation of iristectorigenin A from its structurally similar homologues by ionic-liquid-based ultrasound-assisted extraction and the subsequent screening and isolation of potential α-glucosidase inhibitors via ultrafiltration and semipreparative high-performance liquid chromatography. Ionic-liquid-based ultrasound-assisted extraction was successfully applied to the extraction of tectorigenin, iristectorigenin A, irigenin, and irisflorentin from Belamcanda chinensis. The optimum conditions for the efficient extraction of isoflavones were determined as 1.0 M 1-ethyl-3-methylimidazolium tetrafluoroborate with extraction time of 30 min and a solvent to solid ratio of 30 mL/g. Ultrafiltration with liquid chromatography and mass spectrometry was applied to screen and identify α-glucosidase inhibitors from B. chinensis, followed by the application of semipreparative high-performance liquid chromatography to separate and isolate the active constituents. Four major compounds including tectorigenin, iristectorigenin A, irigenin, and irisflorentin were screened and identified as α-glucosidase inhibitors, and then the four active compounds abovementioned were subsequently isolated by semipreparative high-performance liquid chromatography (99.89, 88.97, 99.79, and 99.97% purity, respectively). The results demonstrate that ionic liquid extraction can be successfully applied to the extraction of isoflavones from B. chinensis.

Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid‐phase extraction coupled with two‐dimensional preparative liquid chromatography

“…7), and all compounds were found to be more than 97% pure and had similar UV spectra. The isolated compounds 1-8 were characterized by 1 H NMR and 13…”

“…Preparative HPLC (prep‐HPLC) is one of the most efficient purification techniques, and provides many advantages in the separation of natural compounds, such as high efficiency, high resolution, and good reproducibility . Accordingly, prep‐HPLC has drawn increasing attention from many phytochemists and pharmaceutical companies.…”

Determination of Ferulic Acid in Angelica sinensis by Temperature-Controlled Hydrophobic Ionic Liquids-Based Ultrasound/Heating-Assisted Extraction Coupled with High Performance Liquid Chromatography

“…Recently, ionic liquids (ILs) have become promising alternatives to the traditional organic solvents employed in sample preparation because of their unique properties, such as negligible vapor pressure, high thermal stability, non-flammability and good solubility for many inorganic and organic compounds [17]. Therefore, ILs have been extensively applied as substituents for conventional organic solvents in various extraction processes including salt-induced liquid-liquid extraction (SI-LLE) [18], dispersive liquid-phase microextraction (DLPME) [19], microwave-assisted extraction (MAE) [20], pressurized liquid extraction [21], ultrasonic-assisted extraction [22][23][24] and temperature-controlled liquid phase micro-extraction (TC-LPME) [25][26][27][28]. In this context, hydrophobic ILs are usually used to extract liquid samples, while hydrophilic ILs are mostly used to extract solid samples.…”

Screening and identification of α‐glucosidase inhibitors from Shenqi Jiangtang Granule by ultrafiltration liquid chromatography and mass spectrometry

“…It is necessary for the characterization of compounds present at low concentrations in complex matrixes without complicated purification procedures. Until now, most reports on ultrafiltration LC-MS, especially for the screening of α-glucosidase inhibitors, focus on single herb extracts, such as Radix Astragali, Radix Salviae miltiorrhizae, Folium Hawthorn, and P. ginseng [8][9][10][11][12]. Jiang et al screened out seven α-glucosidase inhibitors from the flavonoid extract of Radix Astragali by ultrafiltration [8].…”