Ionic mechanisms of the rapid (nicotinic) phase of acetylcholine response in identified Planobarius corneus neurones

Katchman, Alexander N.; Zeimal, E.V.

doi:10.1016/0006-8993(82)91232-x

Cited by 14 publications

(4 citation statements)

References 22 publications

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“…The loop diuretic furosemide is well-established as an inhibitor of the Na+-K+-2Cl-co-transport system (Geck & Heinz, 1986). It has also been reported that furosemide inhibits neurotransmitter-activated chloride currents: GABA responses in frog spinal motoneurones (Nicoll, 1978) and acetylcholine responses in molluscan neurones (Katchman & Zeimal, 1982). In Nicoll's and in the present experiments little or no change in agonist reversal potential during the blocking effect of furosemide was observed.…”

Section: Ionic Basis Of Glu Responsesupporting

confidence: 68%

“…The potency of furosemide to inhibit chloride-dependent responses evoked by GABA in frog spinal motoneurones (Nicoll, 1978) and by acetylcholine in isolated neurones of the mollusc Planorbarius corneus (Katchman & Zeimal, 1982) was shown in earlier work. In our experiments (n = 4) furosemide (0-1 mM) reduced or eliminated completely the fast inward current evoked by Glu application ( Fig.…”

Section: Ionic Basis Of the Action Of Glutamate And Glutamate Agonistsmentioning

confidence: 84%

“…In some experiments the potassium concentration was increased twofold (3-2 mM) with a corresponding decrease of sodium content, or sodium content was doubled (addition of 25 mM-Na2S04). Increased osmolarity of the latter solution (1-7 times) could be neglected since it is known that a prominent increase in osmolarity does not influence responses of these mollusc neurones (Katchman & Zeimal, 1982). The temperature was maintained at 20 +1°C.…”

Section: Perfusion Techniques and Solutionsmentioning

confidence: 99%

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Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.

Bolshakov

Gapon

1991

The Journal of Physiology

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SUMMARY1. The membrane currents evoked by glutamate agonists on isolated and identified neurones of molluscan pedal ganglia were investigated using the voltage clamp technique.2. The fast chloride current (Er (reversal potential) =-41 mV) evoked in a Ped-9 neurone by application of glutamate, quisqualate and ibotenic acid could be blocked by furosemide (0-1 mM). The slow potassium current (Er = -85 mV) evoked in Ped-8 and Ped-9 neurones by glutamate, quisqualate and kainate could be blocked by tetraethylammonium (50 /M).3. N-Methyl-D-aspartate (NMDA) and a-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) failed to induce a response in neurones studies.4. The spider venoms argiopine and argiopinine III (50-500 nM) selectively inhibited quisqualate-induced potassium current, but had no influence on glutamate-, ibotenate-or quisqualate-induced chloride and kainate-induced potassium currents. Glutamate-induced potassium current was partially inhibited by argiopine and argiopinine III.5. The existence of several types of distinct glutamate receptors was confirmed in cross-desensitization experiments, and a lack of interaction was observed between quisqualate and kainate.6. Potassium currents induced both by quisqualate and kainate strongly depended on temperature and could be blocked by pertussis toxin. Intracellular injection of the calcium chelator, EGTA, did not affect quisqualate and kainate responses.7. In neurones loaded with non-hydrolysable GTP analogues, GTP-y-S (guanosine-5'-O-(3-thio)triphosphate) or GppNHp (5'-guanylylimidodiphosphate), the potassium current was gradually induced in the absence of agonists. As this current progressed, the magnitude of the glutamate-or kainate-evoked current transients became smaller and finally negligible. The GTP-y-S-induced current was not inhibited by argiopine.

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Section: Ionic Basis Of Glu Responsesupporting

confidence: 68%

Section: Ionic Basis Of the Action Of Glutamate And Glutamate Agonistsmentioning

confidence: 84%

Section: Perfusion Techniques and Solutionsmentioning

confidence: 99%

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Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.

Bolshakov

Gapon

1991

The Journal of Physiology

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“…It has also been reported that furosemide inhibits neurotransmitter-activated Cl-conductances in neurones (Nicoll, 1978;Katchman & Zeimal, 1982) and Ca2l-activated Cl-channels in lacrimal glands (Evans, Marty, Tan & Trautman, 1986). Figure 13 shows this not to be the case for the apical Cl-conductance of Necturus enterocytes, where furosemide, used at a concentration of 10-3 M, had no effect on the value of AEm induced by a lowCl-mucosal solution.…”

Section: Effects Of Chloride Removal On Membrane Potentialmentioning

confidence: 99%

A chloride conductance activated by adenosine 3',5'‐cyclic monophosphate in the apical membrane of Necturus enterocytes.

Giráldez

Sepúlveda

Sheppard

1988

The Journal of Physiology

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SUMMARY1. Intracellular potentials, C1-activity and membrane resistances were measured in Necturus small intestinal epithelium during C1-replacement experiments using conventional or Cl--selective double-barrelled microelectrodes. A Cl-conductance, located in the apical membrane and activated by cyclic nucleotides is demonstrated by ion-substitution experiments.2. The mean mucosal membrane potential (Em) was -35-5 mV. Removal of C1-from the mucosal medium by replacement with gluconate, evoked a sudden depolarization of Em and an immediate increase in the fractional resistance of the mucosal membrane (f(Rm)). The size of the change in Em varied between 3 and 65 mV, corresponding to Cl-to K+ permeability ratios between 0-2 and 20. It was inversely related to the initial f(Rm), which ranged from 0 04 to 0 50.3. Prolonged incubation in low-Cl-solutions led to a reversal of the initial depolarization and to a sustained hyperpolarization accompanied by a marked increase in f(Rm). The new value of Em was close to the K+ equilibrium potential, consistent with a depletion of cellular Cl-and the preponderance of a K+ membrane permeability in the absence of Cl-. This emphasizes the role of Cl-in establishing Em. 4. Removal of mucosal Cl-produced a fast decrease in intracellular Cl-, as measured with Cl--selective microelectrodes. The efflux was consistent with electrodiffusion across the mucosal membrane. Changes in Em paralleled changes in intracellular Cl-activity, indicating the presence of a large Cl-conductance.5. Dibutyryl cyclic AMP or forskolin produced a slow depolarization, a decrease in f(Rm) and an increased change in intracellular potential in low mucosal Cl-which on average corresponds to an approximately 15-fold increase in the relative Clpermeability. These results are consistent with an activation of apical Clconductance.6. The selectivity of Cl-channels of Necturus enterocytes to different anions was obtained from potential measurements. The sequence of permeabilities was SCN-> I-3 Br-> N03-> Cl-> HC03-> gluconate. This is consistent with a model involving a weak interaction of the anions with the selectivity filter.F. GIRALDEZ, F. V. SEPULVEDA AND D. N. SHEPPARD 7. The selectivity of the anion conductance was maintained after activation with cyclic nucleotides, suggesting a single channel for the permeation of the different anions tested, rather than parallel channels.8. Derivatives of 9-anthracene which are potent inhibitors of Cl-channels in other systems failed to block the apical Cl-conductance of Necturus enterocytes. Chloride conductance was also insensitive to furosemide.9. The Cl-channels described here can coexist within a single enterocyte with Na+-dependent amino acid carriers, as demonstrated by the simultaneous presence of depolarizations in response to 0 Cl-conditions and to L-alanine in the same cells. No apparent relation was observed between the size of the two effects.10. The present results provide the first direct evidence for the presence of a Clconductance in the apical membrane o...

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Molluscan ligand-gated ion-channel receptors

Darlison

Hutton²,

Harvey

1993

Comparative Molecular Neurobiology

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Ionic mechanisms of the rapid (nicotinic) phase of acetylcholine response in identified Planobarius corneus neurones

Cited by 14 publications

References 22 publications

Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.

Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.

A chloride conductance activated by adenosine 3',5'‐cyclic monophosphate in the apical membrane of Necturus enterocytes.

Molluscan ligand-gated ion-channel receptors

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