Ionized jet deposition of antimicrobial and stem cell friendly silver-substituted tricalcium phosphate nanocoatings on titanium alloy

Graziani, Gabriela; Barbaro, Kátia Cristina; Фадеева, И. В.; Ghezzi, Daniele; Fosca, Marco; Sassoni, Enrico; Vadalà, Gianluca; Cappelletti, Martina; Valle, Francesco; Baldini, Nicola; Rau, Julietta V.

doi:10.1016/j.bioactmat.2020.12.019

Cited by 25 publications

(42 citation statements)

References 57 publications

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“…Notably, HC-0.1 showed antibacterial activity even though the Ag content (9.9 × 10 −4 wt %) was remarkably lower than those in other reported scaffolds (0.08−20 wt %), 18,22,23,25,26,31−33 and the antibacterial activity of the reported scaffolds was quite weak when the Ag content was <1 wt %. 32,33 This superiority of HC-0.1 seems to be due to its honeycomb framework structure because bacteria and necrotic bone were eliminated from the CAp HC scaffolds without Ag 3 PO 4 modification (HC-0) through their channels. This suggests that a honeycomb structure that possesses uniaxial channels perfectly penetrating the scaffold is inherently appropriate for the prevention of bacterial adhesion and growth.…”

Section: ■ Discussionmentioning

confidence: 99%

Antibacterial Honeycomb Scaffolds for Achieving Infection Prevention and Bone Regeneration

Hayashi

Shimabukuro

Ishikawa

2022

ACS Appl. Mater. Interfaces

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Surgical site infection (SSI) is a severe complication associated with orthopedic bone reconstruction. For both infection prevention and bone regeneration, the framework surface of osteoconductive and bioresorbable scaffolds must be locally modified by minimum antibacterial substances, without sacrificing the osteoconductivity of the scaffold framework. In this study, we fabricated antibacterial honeycomb scaffolds by replacing carbonate apatite, which is the main component of the scaffold, with silver phosphate locally on the scaffold surface via dissolution–precipitation reactions. When the silver content was 9.9 × 10–4 wt %, the honeycomb scaffolds showed antibacterial activity without cytotoxicity and allowed cell proliferation, differentiation, and mineralization. Furthermore, the antibacterial honeycomb scaffolds perfectly prevented bacterial infection in vivo in the presence of methicillin-resistant Staphylococcus aureus, formed new bone at 2 weeks after surgery, and were gradually replaced with a new bone. Thus, the antibacterial honeycomb scaffolds achieved both infection prevention and bone regeneration. In contrast, severe infection symptoms, including abscess formation, osteolytic lesions, and inflammation, occurred 2 weeks after surgery when honeycomb scaffolds without silver phosphate modification were implanted. Nevertheless, the unmodified honeycomb scaffolds eliminated bacteria and necrotic bone through their scaffold channels, resulting in symptom improvement and bone formation. These results suggest that the honeycomb structure is inherently effective in hindering bacterial growth. This novel insight may contribute to the development of antibacterial scaffolds. Moreover, our modification method is useful for providing antibacterial activity to various biomaterials.

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Section: ■ Discussionmentioning

confidence: 99%

Antibacterial Honeycomb Scaffolds for Achieving Infection Prevention and Bone Regeneration

Hayashi

Shimabukuro

Ishikawa

2022

ACS Appl. Mater. Interfaces

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“…Similarly, the Ti implant with formed TiO 2 nanotubes showed antioxidant and immunomodulation functions for the promotion of bone integration with implants in diabetic rats . In addition, the introduction of nutrient elements or enzymes to the implant was also constructed to reduce the ROS level in the surgical area to help the remodeling of bone. , Moreover, the introduction of silver nanoparticles or metal ions or graphene oxide to the implant is fabricated to enable the antibacterial ability of implants for enhanced osseointegration. , However, limited reports mainly focus on the modulation of glucose level or ROS degree of the diabetic environment to enhance osteogenic capacity for the improved integration effect of implants. , Therefore, to improve the osseointegration ability of implants, the microenvironment of excessive oxidative stress affected vasculogenic disorders and potential infection risk in diabetic bone defect should be emphasized in further research on the implants.…”

Section: Introductionmentioning

confidence: 99%

“…31,32 Moreover, the introduction of silver nanoparticles or metal ions or graphene oxide to the implant is fabricated to enable the antibacterial ability of implants for enhanced osseointegration. 33,34 However, limited reports mainly focus on the modulation of glucose level or ROS degree of the diabetic environment to enhance osteogenic capacity for the improved integration effect of implants. 30,32 Therefore, to improve the osseointegration ability of implants, the microenvironment of excessive oxidative stress affected vasculogenic disorders and potential infection risk in diabetic bone defect should be emphasized in further research on the implants.…”

Section: Introductionmentioning

confidence: 99%

Decorated Polyetheretherketone Implants with Antibacterial and Antioxidative Effects through Layer-by-Layer Nanoarchitectonics Facilitate Diabetic Bone Integration with Infection

Huang

Lin

Bai

et al. 2022

ACS Appl. Mater. Interfaces

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Patients suffering diabetic bone defects still need some new and effective strategies to achieve enhanced prognostic effects. Although medical implants are the common treatment of bone defects, the excessive oxidative stress and high risk of bacterial infection in diabetes mellitus lead to a higher risk of implant failure. To improve the healing ability of diabetic bone defects, herein, polyetheretherketone (PEEK) was modified through a developed layer-by-layer (LBL) construction strategy to obtain multifunctional PEEK (SP@(TA-GS/PF)*3) by the assembly of tannic acid (TA), gentamicin sulfate (GS) and Pluronic F127 (PF127) on the basis of prepared porous PEEK through sulfonation (SPEEK). The prepared SP@(TA-GS/PF)*3 exhibited sustained antimicrobial activity and enhanced the differentiation of osteoblast (MC3T3-E1) for needed osteogenesis. Moreover, SP@(TA-GS/PF)*3 scavenged excessive oxidative stress to promote the growth of H 2 O 2 damaged HUVEC with enhanced secretion of VEGF for neovascularization. In addition, the remarkable in vivo outcomes of angiogenesis and osseointegration were revealed by the subcutaneous implant model and bone tissue implant model in diabetic rats, respectively. The in vitro and in vivo results demonstrated that modified PEEK with multifunction can be an attractive tool for enhancing bone integration under diabetic conditions, underpinning the clinical application potential of modified implants for diabetic osseointegration.

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“…In addition to the modulation of physicochemical characteristics of β -TCP, metal-substituted TCPs provide new advanced properties to the materials. Antibacterial characteristics can be imparted by Cu 2+ , Fe 3+ , Zn 2+ , and Ag + ions [ 12 , 14 , 15 , 16 ], and the biocompatibility properties and, namely, cell proliferation on the materials surface can be improved by Fe 3+ and Sr 2+ ions substitution [ 5 , 14 , 17 , 18 , 19 ]. Mg-doped β -TCP biomaterials are able to enhance the cell proliferation and viability of human bone marrow-derived mesenchymal stem cells [ 20 ] and the differentiation of hBMSCs into osteoblasts [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Synthesis Conditions on Gadolinium-Substituted Tricalcium Phosphate Ceramics and Its Physicochemical, Biological, and Antibacterial Properties

et al. 2022

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Gadolinium-containing calcium phosphates are promising contrast agents for various bioimaging modalities. Gadolinium-substituted tricalcium phosphate (TCP) powders with 0.51 wt% of gadolinium (0.01Gd-TCP) and 5.06 wt% of (0.1Gd-TCP) were synthesized by two methods: precipitation from aqueous solutions of salts (1) (Gd-TCP-pc) and mechano-chemical activation (2) (Gd-TCP-ma). The phase composition of the product depends on the synthesis method. The product of synthesis (1) was composed of β-TCP (main phase, 96%), apatite/chlorapatite (2%), and calcium pyrophosphate (2%), after heat treatment at 900 °C. The product of synthesis (2) was represented by β-TCP (main phase, 73%), apatite/chlorapatite (20%), and calcium pyrophosphate (7%), after heat treatment at 900 °C. The substitution of Ca2+ ions by Gd3+ in both β-TCP (main phase) and apatite (admixture) phases was proved by the electron paramagnetic resonance technique. The thermal stability and specific surface area of the Gd-TCP powders synthesized by two methods were significantly different. The method of synthesis also influenced the size and morphology of the prepared Gd-TCP powders. In the case of synthesis route (1), powders with particle sizes of tens of nanometers were obtained, while in the case of synthesis (2), the particle size was hundreds of nanometers, as revealed by transmission electron microscopy. The Gd-TCP ceramics microstructure investigated by scanning electron microscopy was different depending on the synthesis route. In the case of (1), ceramics with grains of 1–50 μm, pore sizes of 1–10 µm, and a bending strength of about 30 MPa were obtained; in the case of (2), the ceramics grain size was 0.4–1.4 μm, the pore size was 2 µm, and a bending strength of about 39 MPa was prepared. The antimicrobial activity of powders was tested for four bacteria (S. aureus, E. coli, S. typhimurium, and E. faecalis) and one fungus (C. albicans), and there was roughly 30% of inhibition of the micro-organism’s growth. The metabolic activity of the NCTC L929 cell and viability of the human dental pulp stem cell study demonstrated the absence of toxic effects for all the prepared ceramic materials doped with Gd ions, with no difference for the synthesis route.

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Ionized jet deposition of antimicrobial and stem cell friendly silver-substituted tricalcium phosphate nanocoatings on titanium alloy

Cited by 25 publications

References 57 publications

Antibacterial Honeycomb Scaffolds for Achieving Infection Prevention and Bone Regeneration

Antibacterial Honeycomb Scaffolds for Achieving Infection Prevention and Bone Regeneration

Decorated Polyetheretherketone Implants with Antibacterial and Antioxidative Effects through Layer-by-Layer Nanoarchitectonics Facilitate Diabetic Bone Integration with Infection

Influence of Synthesis Conditions on Gadolinium-Substituted Tricalcium Phosphate Ceramics and Its Physicochemical, Biological, and Antibacterial Properties

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