IP3 Mediates Nitric Oxide–Guanosine 3′,5′-Cyclic Monophosphate (NO-cGMP)-Induced Isoflavone Accumulation in Soybean Sprouts under UV-B Radiation

Abstract

In this study, to investigate the role of inositol 1,4,5-trisphosphate (IP3) in nitric oxide-guanosine 3',5'-cyclic monophosphate (NO-cGMP)-induced isoflavone accumulation in soybean sprouts under UV-B radiation, the sprouts were treated with donors and inhibitors of NO and cGMP as well as IP3 inhibitor. Results showed that NO, with cGMP as a second messenger, stimulates IP3 accumulation under UV-B radiation. Consistent with the increase in IP3 content, the up-regulation of gene and protein expression of phosphoinositide-specific phospholipase C (PI-PLC) in response to sodium nitroprusside (SNP) (exogenous NO donor) and 8-Br-cGMP (cGMP analogue) was also observed. In addition, protein kinase G (PKG) participated in NO-cGMP-induced IP3 production. IP3 induced by the NO-cGMP pathway was involved in isoflavone synthesis by elevating the activity and gene and protein expressions of chalcone synthase (CHS) and isoflavone synthase (IFS). Overall, IP3 mediates NO-cGMP-induced isoflavone accumulation in soybean sprouts under UV-B stress.

Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress

“…The membranes were incubated with primary antibody (anti-CaMK, anti-CDPK and anti-CCaMK, own-made) overnight at 4 °C and then washed 4 times for 5 min each with TBST and were then incubated with secondary goat polyclonal antibody conjugated to horseradish peroxidase (goat anti-mouse IgG, 1:5000; goat anti-rabbit IgG, 1:5000, Thermo Pierce) for 1 h at 25 °C. The subsequent experiment was carried out according to the method of Jiao et al (Jiao, Wang, Yang, Tian, & Gu, 2016).…”

Occurrence of isoflavones in soybean sprouts and strategies to enhance their content: A review

“…Some studies have tried to establish the mechanistic interactions between external irradiation and isoflavone biosynthesis. As shown in Figure 4, nitric oxide (NO) has been reported to induce isoflavone biosynthesis under UV‐B radiation (Jiao & Gu, 2019a, 2019c, 2019d; Jiao & Liu, 2021; Jiao, Wang, et al., 2016; Jiao, Yang, & Gu, 2016; Jiao, Yang, Zhou, et al., 2016; Jiao et al., 2017; Jiao, Yang, & Gu, 2018; Jiao, Yang, et al., 2018). As a response to UV‐B stress, plants produce NO, which in turn triggers an increase in guanylyl cyclase (GC) activity, increasing the production of guanosine 3′,5′‐cyclic monophosphate (cGMP).…”

Comparative Analysis of Phenolic Compound Profiles, Antioxidant Capacities, and Expressions of Phenolic Biosynthesis-Related Genes in Soybean Microgreens Grown under Different Light Spectra

“…For instance, ultraviolet B (UV-B) light was shown to significantly increase isoflavone content in soybean sprouts. 7,[29][30][31] Similarly, UV-A was reported to induce anthocyanin biosynthesis in hypocotyls of soybean sprouts. 32 Besides, the antioxidant capacity of germinated soybean was also reported to be subjected by light treatments (such as fluorescent light and UV-B).…”