IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation

Kumari, Pratibha; Dahiya, Pradeep; Livanos, Pantelis; Zergiebel, Luise; Kölling, Malte; Poeschl, Yvonne; Stamm, Gina; Hermann, Arvid; Abel, Steffen; Müller, Sabine; Bürstenbinder, Katharina

doi:10.1038/s41477-021-00923-z

Cited by 51 publications

(34 citation statements)

References 45 publications

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“…This mutant was recently reported to have distorted cell division planes in roots, along with alterations in the organization of the MT cytoskeleton. 35 Likewise, in 35% of the analyzed embryos ( Figure S3 B), the mutant shows a shift in division plane orientation during different stages, which varies from subtle to more severe defects ( Figure S3 C). The divisions leading to 8-cell embryos are symmetric and use the minimal surface area, similar to wild-type embryos ( Figures 5 D and 5E).…”

Section: Resultsmentioning

confidence: 95%

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Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

et al. 2021

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Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo Graphical abstract Highlights d Auxin responses regulate directional cell expansion in Arabidopsis embryos d Cell shape and division orientation are tightly coupled d Transcriptome analysis identifies MT-associated IQD proteins in division control d Cytoskeletal dynamics control division orientation

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Section: Resultsmentioning

confidence: 95%

“…All lines were backcrossed at least once with Col-0 and subsequently iqd6 , iqd7 , and iqd8 were crossed among themselves to generate iqd678 triple mutant. 35 …”

Section: Methodsmentioning

confidence: 99%

Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

et al. 2021

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“…We hypothesize that IQD9, KLCR1 and TRM4 could be an integral part of an expanding group of proteins (Polko & Kieber, 2019) that support PM‐bound CSC movement along the orientation of MT tracks. IQDs are hypothesized to function as scaffolds that recruit other proteins and may be modified by TON1/TRM/PP2A (TTP)‐mediated dephosphorylation (Kumari et al ., 2021). Via IQ67‐domain‐mediated calmodulin binding (Abel et al ., 2005), IQDs could also participate in Ca 2+ signaling to ultimately influence plant cell wall dynamics.…”

Section: Discussionmentioning

confidence: 99%

Microtubule‐associated IQD9 orchestrates cellulose patterning in seed mucilage

et al. 2022

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Arabidopsis seeds release large capsules of mucilaginous polysaccharides, which are shaped by an intricate network of cellulosic microfibrils. Cellulose synthase complexes are guided by the microtubule cytoskeleton, but it is unclear which proteins mediate this process in the seed coat epidermis.Using reverse genetics, we identified IQ67 DOMAIN 9 (IQD9) and KINESIN LIGHT CHAIN-RELATED 1 (KLCR1) as two highly expressed genes during seed development and comprehensively characterized their roles in cell wall polysaccharide biosynthesis.Mutations in IQD9 as well as in KLCR1 lead to compact mucilage capsules with aberrant cellulose distribution, which can be rescued by transgene complementation. IQD9 physically interacts with KLCR1 and localizes to cortical microtubules (MTs) to maintain their organization in seed coat epidermal (SCE) cells. IQD9 as well as a previously identified TONNEAU1 (TON1) RECRUITING MOTIF 4 (TRM4) protein act to maintain cellulose synthase velocity.Our results demonstrate that IQD9, KLCR1 and TRM4 are MT-associated proteins that are required for seed mucilage architecture. This study provides the first direct evidence that members of the IQD, KLCR and TRM families have overlapping roles in cell wall biosynthesis. Therefore, SCE cells provide an attractive system to further decipher the complex genetic regulation of polarized cellulose deposition.

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“…iqd678 triple mutants have PPB formation defects and fail to recruit POK1 to the division site in cells lacking PPBs. However, POK1 localization to the division site in iqd678 mutants recovers during telophase to wild-type levels (Kumari et al, 2021). We speculate that this IQD6-8 independent recruitment may depend on TAN1.…”

Section: Discussionmentioning

confidence: 99%

The localization of PHRAGMOPLAST ORIENTING KINESIN1 at the division site depends on two microtubule binding proteins TANGLED1 and AUXIN-INDUCED-IN-ROOT-CULTURES9 in Arabidopsis

Mills¹,

Morris

Rasmussen

2022

Preprint

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Proper plant growth and development requires spatial coordination of cell divisions. Two unrelated microtubule-binding proteins, TANGLED1 (TAN1) and AUXIN-INDUCED-IN-ROOT-CULTURES9 (AIR9), are together required for normal growth and division-plane orientation in Arabidopsis. tan1 air9 double mutants have synthetic growth and division-plane orientation defects while single mutants lack obvious defects. Here we show that the division-site localized protein, PHRAGMOPLAST-ORIENTING-KINESIN1 (POK1), was aberrantly lost from the division site during metaphase and telophase in tan1 air9 mutants. Since TAN1 and POK1 interact via the first 132 amino acids of TAN1 (TAN1-1-132), we assessed its localization and function in the tan1 air9 double mutant. TAN1-1-132 rescued tan1 air9 mutant phenotypes and localized to the division site in telophase. However, replacing six amino-acid residues within TAN1-1-132 that disrupts POK1-TAN1 interaction in the yeast-two-hybrid system caused loss of both rescue and division-site localization of TAN1-1-132 in tan1 air9 mutants. Full-length TAN1 with the same alanine substitutions had defects in phragmoplast guidance and reduced TAN1 and POK1 localization at the division site but rescued most tan1 air9 mutant phenotypes. Together, these data suggest that TAN1 and AIR9 are required for POK1 localization, and yet unknown proteins may stabilize TAN1-POK1 interactions.

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IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation

Cited by 51 publications

References 45 publications

Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

Microtubule‐associated IQD9 orchestrates cellulose patterning in seed mucilage

The localization of PHRAGMOPLAST ORIENTING KINESIN1 at the division site depends on two microtubule binding proteins TANGLED1 and AUXIN-INDUCED-IN-ROOT-CULTURES9 in Arabidopsis

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