Irisin pathways in hearts of Type 1 diabetic adult male rats following 6 weeks of moderate and high-volume aerobic exercise on a treadmill

Çelik, Hümeyra; Dursun, Ali Doğan; Tatar, Yakup; Ömercioğlu, Göktuğ; Baştuğ, Metin

doi:10.1007/s11332-022-00924-1

Cited by 1 publication

(2 citation statements)

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“…Second, our study mainly focused on in vivo experiments, which means that we did not examine the specific effects of irisin on different cell types of the heart such as cardiomyocytes, endothelial cells, and fibroblasts, nor did we measure fibrosis markers such as fibronectin and collagen in cardiac tissue. Furthermore, the absence of hemodynamic data, such as blood pressure, is also a limitation as previous studies have revealed the involvement of irisin in the positive impact of exercise on blood pressure in diabetic mice 27,51,52 . Therefore, more studies are required to clarify these issues and to explore the potential of irisin as a novel therapeutic strategy for improving DCM.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the absence of hemodynamic data, such as blood pressure, is also a limitation as previous studies have revealed the involvement of irisin in the positive impact of exercise on blood pressure in diabetic mice. 27 , 51 , 52 Therefore, more studies are required to clarify these issues and to explore the potential of irisin as a novel therapeutic strategy for improving DCM.…”

Section: Discussionmentioning

confidence: 99%

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Exercise ameliorating myocardial injury in type 2 diabetic rats by inhibiting excessive mitochondrial fission involving increased irisin expression and AMP‐activated protein kinase phosphorylation

Wang,

Zhao,

Wang

et al. 2023

Journal of Diabetes

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PurposeThough exercise generates beneficial effects on diabetes‐associated cardiac damage, the underlying mechanism is largely unclear. Therefore, we prescribed a program of 8‐week treadmill training for type 2 diabetes mellitus (T2DM) rats and determined the role of irisin signaling, via interacting with AMP‐activated protein kinase (AMPK), in mediating the effects of exercise on myocardial injuries and mitochondrial fission.MethodsForty 8‐week‐old male Wistar rats were randomly divided into groups of control (Con), diabetes mellitus (DM), diabetes plus exercise (Ex), and diabetes plus exercise and Cyclo RGDyk (ExRg). Ex and ExRg rats received 8 weeks of treadmill running, and the rats in the ExRg group additionally were treated with a twice weekly injection of Cyclo RGDyk, an irisin receptor‐αV/β5 antagonist. At the end of the experiment, murine blood samples and heart tissues were collected and analyzed with methods of ELISA, Western blot, real‐time quantitative polymerase chain reaction, as well as immunofluorescence staining.ResultsExercise effectively mitigated T2DM‐related hyperglycemia, hyperinsulinemia, lipid dysmetabolism, and inflammation, which could be diminished by Cyclo RGDyk treatment. Additionally, exercise alleviated T2DM‐induced myocardial injury and excessive mitochondrial fission, whereas the beneficial effects were blocked by the administration of Cyclo RGDyk. T2DM significantly decreased serum irisin concentrations and fibronectin type III domain‐containing protein 5 (FNDC5)/irisin gene and protein expression levels in the rat heart, whereas exercise could rescue T2DM‐reduced FNDC5/irisin expression. Blocking irisin receptor signaling diminished the exercise‐alleviated mitochondrial fission protein expression and elevated AMPK phosphorylation.ConclusionExercise is effective in mitigating diabetes‐related insulin resistance, metabolic dysfunction, and inflammation. Irisin signaling engages in exercise‐associated beneficial effects on myocardial injury and excessive mitochondrial fission in diabetes rats involving elevated AMPK phosphorylation.

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Section: Discussionmentioning

confidence: 99%