Iron Chlorin e6 Scavenges Hydroxyl Radical and Protects Human Endothelial Cells against Hydrogen Peroxide Toxicity.

Yu, Jeong Sik; Yoon, Sang Hyun; Yang, Ryung

doi:10.1248/bpb.24.1053

Cited by 15 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Free Radical Scavenging Activity Assay Free radical scavenging activity was assayed spectrophotometrically by the cytochrome C reduction method as described by McCord and Fridovich 22) and Yu et al 23) When xanthine oxidase converts xanthine to uric acid, the superoxide anion produced would reduce ferricytochrome C to ferrocytochrome C. Since ferrocytochrome C shows a maximum absorption at 550 nm, the amount of superoxide anion can be evaluated indirectly by spectrophotometric measurement of ferrocytochrome C. Therefore, the superoxide anion scavenging activity of T. chebula can be evaluated using the cytochrome C reduction method. Samples were dissolved in DMSO and diluted to desired concentrations with 50 mM of KH 2 PO 4 (pHϭ7.8) solution.…”

Section: Antioxidant and Free Radical Scavenging Activities Of Terminmentioning

confidence: 99%

Antioxidant and Free Radical Scavenging Activities of Terminalia chebula

Cheng

Lin

et al. 2003

Biological & Pharmaceutical Bulletin

217

125

View full text Add to dashboard Cite

Section: Antioxidant and Free Radical Scavenging Activities Of Terminmentioning

confidence: 99%

Antioxidant and Free Radical Scavenging Activities of Terminalia chebula

Cheng

Lin

et al. 2003

Biological & Pharmaceutical Bulletin

217

125

View full text Add to dashboard Cite

“…14,15 Another powerful oxidant, the hydroxyl radical (OH·), may also be formed under conditions of oxidative stress when hydrogen peroxide interacts with divalent cations in a reaction known as Fenton chemistry. Hydroxyl radicals are highly cytotoxic and are thought to cause cellular dysfunction through oxidative modification of lipids, 16 proteins 17,18 and DNA 19 alike.…”

Section: Vascular Effects Of Rosmentioning

confidence: 99%

Novel isoforms of NADPH oxidase in vascular physiology and pathophysiology

Bengtsson

Gulluyan

Dusting

et al. 2003

Clin Exp Pharma Physio

120

View full text Add to dashboard Cite

1. Vascular cells have evolved to use reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, as signalling molecules. Under physiological conditions, ROS are important regulators of cell cycle, protein kinase activity and gene expression. However, in vascular disease states, such as hypertension and hypercholesterolaemia, excessive production of ROS may overwhelm the anti-oxidant defence mechanisms of cells, resulting in 'oxidative stress', damage to the artery wall and, ultimately, development of atherosclerotic plaques. 2. The primary source of ROS in the vasculature is NADPH oxidase. There appear to be at least three isoforms of NADPH oxidase expressed in the vascular wall, each differing with respect to the flavin-containing catalytic subunit it uses to transfer electrons from NADPH to molecular oxygen. Thus, although endothelial cells and adventitial fibroblasts express a gp91phox-containing NADPH oxidase similar to that originally identified in phagocytes, vascular smooth muscle cells may rely on novel homologues of gp91phox, namely Nox1 and Nox4, to produce superoxide. 3. Controversy remains over which isoform(s) of NADPH oxidase is responsible for the oxidative stress associated with vascular diseases. We and others have shown that although gp91phox mRNA expression is upregulated during atherogenesis in human and animal models, expression of the Nox4 subunit remains unchanged. Nox1 expression is also likely to be increased in diseased arteries; however, its relative level of expression, at least at the mRNA level, appears to be markedly lower than that of the other gp91phox homologues, even after upregulation. 4. Whether these findings suggest that a gp91phox-containing NADPH oxidase is more important than either Nox4 or Nox1 in vascular disease awaits studies examining relative protein expression and enzyme kinetics of each subunit, as well as the effects of targeted gene deletion of each of these gp91phox homologues on atherogenesis.

show abstract

“…Free radical scavenging activity was assayed spectrophotometrically by the cytochrome C reduction method as described by McCord and Fridovich [19] and Yu et al, [35]. When xanthine oxidase converts xanthine to uric acid, the superoxide anion produced would reduce ferricytochrome C to ferrocytochrome C. Since ferrocytochrome C shows maximum absorption at 550 nm, the amount of superoxide anion can be evaluated indirectly by spectrophotometric measurement of ferrocytochrome C. Therefore, the superoxide anion scavenging activity of E. thymifolia can be evaluated using the cytochrome C reduction method.…”

Section: Free Radical Scavenging Activitymentioning

confidence: 99%

Antioxidant and Antiviral Activities of <i>Euphorbia thymifolia </i>L.

et al. 2002

View full text Add to dashboard Cite

The antioxidant and antiviral activities of Euphorbia thymifolia L. (Euphorbiaceae) were investigated in this study. The results showed that all of the fractions (MeOH, CHCl₃, EtOAc, n-butanol and water) and pure compounds (3-O-galloyl-4,6-(S)-HHDP-D-glucose, rugosin B and 1,3,4,6-tetra-O-galloyl-K-β-D-glucose)tested possessed antioxidant activities, with the exception of the organic aqueous fraction in the anti-lipid and anti-superoxide formation assays. The range of IC₅₀ of anti-lipid formation, anti-superoxide formation and free radical scavenging assays for all fractions and pure compounds were 2.81–7.63, 0.03–2.18 and 0.013–2.878 mg/ml, respectively. Electron spin resonance studies showed that water extract and pure compounds of E. thymifolia exhibited superoxide radical and hydroxyl radical scavenging activities. Besides antioxidant activities, 3-O-galloyl-4,6-(S)-HHDP-D-glucose and EtOAc fractionalso showed anti-HSV-2 activity. Thus, E. thymifolia was concluded to possess antioxidant and anti-HSV-2 activities.

show abstract

Iron Chlorin e6 Scavenges Hydroxyl Radical and Protects Human Endothelial Cells against Hydrogen Peroxide Toxicity.

Cited by 15 publications

References 43 publications

Antioxidant and Free Radical Scavenging Activities of Terminalia chebula

Antioxidant and Free Radical Scavenging Activities of Terminalia chebula

Novel isoforms of NADPH oxidase in vascular physiology and pathophysiology

Antioxidant and Antiviral Activities of <i>Euphorbia thymifolia </i>L.

Contact Info

Product

Resources

About