“…The main effect of Fe deprivation from the growth medium (−Fe plants) was the significantly lower leaf N and Fe but also the significantly higher P, K, Ca, Mg and Zn whereas the main effect of high bicarbonate (+HCO 3 plants) was the significantly lower leaf N, P, Fe and Zn and the significantly higher K, Ca, Mg; decreased leaf N, P, Fe concentrations in plants grown with bicarbonate were also observed by Fernandez-Lopez et al (1993), Shi et al (1993a), Bertoni et al (1992) whereas decreased Zn has been only referred to in rice by Forno et al (1975) and Yang et al (1994). Although −Fe or +HCO 3 plants exhibited similar chlorotic symptoms of quite the same intensity, the presence of bicarbonate in the growth medium affected the inorganic composition of the plants differently than the lack of Fe (Hamze and Nimah, 1982;Fernandez-Lopez et al, 1993;Shi et al, 1993a;Cinelli et al, 2003). The fact that leaf N concentration of rootstocks grown without Fe was lower than that of rootstocks with Fe could be ascribed to the decreased activity of nitrate reductase because of the lack of Fe (Wilkinson, 1994, Smolders et al, 1997; in the case of rootstocks grown with bicarbonate the lower leaf N concentration may also be linked to reduced nitrate uptake because of high bicarbonate in the nutrient solution (Bertoni et al, 1992).…”