Iron effect on acetone-butanol fermentation

Junelles, Anne-Marie; Janati‐Idrissi, Rachid; Petitdemange, H.; Gay, Robert

doi:10.1007/bf01571332

Cited by 70 publications

(38 citation statements)

References 11 publications

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“…They reported that at initial pH of 7.0, 1000 mgL −1 of FeSO 4 .7H 2 O was sufficient and gave maximum production rate but at initial pH of 8.5, the needed was about 25 mgL −1 of FeSO 4 .7H 2 O, suggested that iron supplementation depend in both initial pH, microorganisms and another factors that affected by iron concentration. Our results regarding the bacterial growth agreed with Junelles et al [11] , they reported that iron limitation affect the growth and the endproducts of fermentation of Clostridium acetobutylicum. They found under iron limitation (0.2 mgL ) at a pH of 4.8, glucose was fermented to butanol as the major fermentation end product and the final conversion yield of glucose into butanol could be increased from 20-30% by iron limitation.…”

Section: Effect Of Calcium Additionsupporting

confidence: 93%

“…In order to find out the conditions that could made the bacterium to be affected by the presence of iron in fermentation media, Junelles et al [11] pointed out that the hydrogenase specific activity was decreased, carbon and electron flow were affected under iron limitation. Whereas Mongi et al [12] , they stated that at initial pH, the bacterial needed for iron was variant.…”

Section: Effect Of Calcium Additionmentioning

confidence: 99%

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Trace Metal Effect on Hydrogen Production Using C.acetobutylicum

Alshiyab

Kalil²,

Hamid³

et al. 2008

OnLine J. of Biological Sciences

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This study was carried out to study the effect of trace metal addition for hydrogen production The results show that this bacterial need a selected nutrient with selected concentration. The optimum parameters for cultivation were at initial pH of 7.0 and temperature of 30°C. Trace metal addition to the new medium showed that only iron enhanced the H 2 yield. The results showed that 25 mgL −1 FeSO 4 ⋅7H 2 O enhanced the hydrogen yield from 391 mLg −1 to 408 mLg −1 glucose utilized with biomass concentration of 1.38 gL

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Section: Effect Of Calcium Additionsupporting

confidence: 93%

Section: Effect Of Calcium Additionmentioning

confidence: 99%

Trace Metal Effect on Hydrogen Production Using C.acetobutylicum

Alshiyab

Kalil²,

Hamid³

et al. 2008

OnLine J. of Biological Sciences

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“…It is speculated that the intracellular ATP and NADH pools play a crucial role in the clostridial adaptation to changing environmental condition including the pH level (Girbal and Soucaille 1994;Wietzke and Bahl 2012), nutritional composition (Bahl et al 1986;Girbal and Soucaille 1994), and further limitations (e.g. iron (Junelles et al 1988;Vasileva et al 2012)). …”

Section: Discussionmentioning

confidence: 99%

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum—evidence from a mathematical model

Millat

Voigt

Janssen

et al. 2014

Appl Microbiol Biotechnol

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(2014) Coenzyme A-transferaseindependent butyrate re-assimilation in Clostridium acetobutylicum -evidence from a mathematical model. Applied Microbiology and Biotechnology, 98 (21 A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum -Evidence from a mathematical model Abstract: The hetero-dimeric CoA-transferase CtfA/B is believed to be crucial for the metabolic transition from acidogenesis to solventogenesis in Clostridium acetobutylicum as part of the industrial-relevant acetone-butanol-ethanol (ABE) fermentation. Here, the enzyme is assumed to mediate re-assimilation of acetate and butyrate during a pH-induced metabolic shift and to faciliate the first step of acetone formation from acetoacetyl-CoA. However, recent investigations using phosphate-limited continuous cultures have questioned this common dogma.To adress the emerging experimental discrepancies, we investigated the mutant strain Cac-ctfA398s::CT using chemostat cultures. As a consequence of this mutation, the cells are unable to express functional ctfA and are thus lacking CoA-transferase activity. A mathematical model of the pH-induced metabolic shift, which was recently developed for the wild type, is used to analyse the observed behaviour of the mutant strain with a focus on re-assimilation activities for the two produced acids.Our theoretical analysis reveals that the ctfA mutant still re-assimilates butyrate, but not acetate. Based upon this finding, we conclude that C. acetobutylicum possesses a CoA-tranferase-independent butyrate uptake mechanism that is activated by decreasing pH levels. Furthermore, we observe that butanol formation is not inhibited under our experimental conditions, as suggested by previous batch culture experiments. In concordance with recent batch experiments, acetone formation is abolished in chemostat cultures using the ctfa mutant.

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“…The alteration of the electron flow by carbon monoxide, a reversible inhibitor of the hydrogenase, can also induce the shift from an acidogenic fermentation to an alcohologenic one (9,20,25,27): alcohol (butanol and ethanol) and lactate production at very high specific rates is obtained, without acetone and little or no acetate and butyrate formation. The decrease of hydrogenase activity or concentration by iron limitation conditions (19) specifically yields butanol and ethanol as the major fermentation end products. It has also been shown, in continuous cultures at low pH, that phosphate limitation, nitrogen limitation, and glucose excess are conditions under which primarily solvents are produced by C. acetobutylicum (3,4,35).…”

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confidence: 99%

Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol

1994

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The metabolism of Clostridium acetobutylicum was manipulated, at neutral pH and in chemostat culture, by changing the overall degree of reduction of the substrate, using mixtures of glucose and glycerol. Cultures grown on glucose alone produced only acids, and the intracellular enzymatic pattern indicated the absence of butyraldehyde dehydrogenase activity and very low levels of coenzyme A-transferase, butanol, and ethanol dehydrogenase activities. In contrast, cultures grown on mixtures of glucose and glycerol produced mainly alcohols and low levels of hydrogen. The low production of hydrogen was not associated with a change in the hydrogenase level but was correlated with the induction of a ferredoxin-NAD reductase and a decreased level of NADH-ferredoxin reductase. The production of alcohols was related to the induction of a NAD-dependent butyraldehyde dehydrogenase and to higher expression of NAD-dependent ethanol and butanol dehydrogenases. The coenzyme A-transferase was poorly expressed, and thus no acetone was produced. These changes in the enzymatic pattern, obtained with cultures grown on a mixture of glucose and glycerol, were associated with a 7-fold increase of the intracellular level of NADH and a 2.5-fold increase of the level of ATP.The complex anaerobic metabolism of Clostridium acetobutylicum has been studied in considerable detail in recent years, but the factors involved in triggering the metabolic shift, and the physiological state associated with the transition from the acidogenic to solventogenic phase, are still not totally understood.In a number of studies, the effects of electron flow regulation, nutrient limitation, and end product accumulation on the onset and maintenance of solvent production have been investigated. In typical batch fermentations, initiation of solventogenesis is associated with a pH-acid effect. Lowering the intracellular pH and thereby increasing the concentration of undissociated butyric acid has a positive effect on the production of acetone and butanol (2,13,14,28,29,38). The alteration of the electron flow by carbon monoxide, a reversible inhibitor of the hydrogenase, can also induce the shift from an acidogenic fermentation to an alcohologenic one (9,20,25,27): alcohol (butanol and ethanol) and lactate production at very high specific rates is obtained, without acetone and little or no acetate and butyrate formation. The decrease of hydrogenase activity or concentration by iron limitation conditions (19) specifically yields butanol and ethanol as the major fermentation end products. It has also been shown, in continuous cultures at low pH, that phosphate limitation, nitrogen limitation, and glucose excess are conditions under which primarily solvents are produced by C. acetobutylicum (3,4,35).In this study, the metabolic flexibility of C. acetobutylicum was studied in chemostat culture by increasing the NAD(P)H pressure, using mixtures of glucose and a more reduced chemical like glycerol. The pH of the culture was maintained at 6.5 so that no changes could be r...

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Iron effect on acetone-butanol fermentation

Cited by 70 publications

References 11 publications

Trace Metal Effect on Hydrogen Production Using C.acetobutylicum

Trace Metal Effect on Hydrogen Production Using C.acetobutylicum

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum—evidence from a mathematical model

Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol

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