Iron homeostasis in insects: Insights from <i>Drosophila</i> studies

Tang, Xiaona; Zhou, Bing

doi:10.1002/iub.1211

Cited by 85 publications

(81 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In constrast to mammalian ferritin, which is predominantly found in the cytosol, Drosophila ferritin binds iron in the early secretory compartments and is then secreted into the circulation system (Mandilaras et al, 2013; Tang and Zhou, 2013a). This process is central for systemic iron supply as well as tissue iron detoxification (Tang and Zhou, 2013b).…”

Section: Introductionmentioning

confidence: 99%

The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster

Xiao

Wan

Fan

et al. 2014

eLife

Self Cite

View full text Add to dashboard Cite

The intracellular iron transfer process is not well understood, and the identity of the iron transporter responsible for iron delivery to the secretory compartments remains elusive. In this study, we show Drosophila ZIP13 (Slc39a13), a presumed zinc importer, fulfills the iron effluxing role. Interfering with dZIP13 expression causes iron-rescuable iron absorption defect, simultaneous iron increase in the cytosol and decrease in the secretory compartments, failure of ferritin iron loading, and abnormal collagen secretion. dZIP13 expression in E. coli confers upon the host iron-dependent growth and iron resistance. Importantly, time-coursed transport assays using an iron isotope indicated a potent iron exporting activity of dZIP13. The identification of dZIP13 as an iron transporter suggests that the spondylocheiro dysplastic form of Ehlers–Danlos syndrome, in which hZIP13 is defective, is likely due to a failure of iron delivery to the secretory compartments. Our results also broaden our knowledge of the scope of defects from iron dyshomeostasis.DOI: http://dx.doi.org/10.7554/eLife.03191.001

show abstract

Section: Introductionmentioning

confidence: 99%

The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster

Xiao

Wan

Fan

et al. 2014

eLife

Self Cite

View full text Add to dashboard Cite

show abstract

“…This deposition leads to changes in the development of eye and brain tissues, causing malformation of these structures 25 , demonstrating the toxic effects of the iron excess in tissues. To avoid these deleterious effects in a diet rich in iron, usually there is an increase the expression of ferritin gene and protein [26][27][28] . This mechanism was observed by Georgieva and colleagues 28 in D. melanogaster when individuals fed on media supplement with iron in which increased ferritin mRNA levels was detected in larvae, pupae and imagoes.…”

Section: Discussionmentioning

confidence: 99%

Functional genomics of the parasitic habits of blowflies and botflies

Cardoso

Deszo

Torres

2019

Preprint

View full text Add to dashboard Cite

Infestation by dipterous larvaemyiasis -is a major problem for livestock industries worldwide and can cause severe economic losses. The Oestroidea superfamily is an interesting model to study the evolution of myiasis-causing flies because of the diversity of parasitism strategies among closely-related species. These flies are saprophagous, obligate parasites, or facultative parasites and can be subdivided into cutaneous, nasopharyngeal, traumatic, and furuncular. We expect that closely-related species have genetic differences that lead to the diverse parasitic strategies. To identify genes with such differences, we used gene expression and coding sequence data from five species (Cochliomyia hominivorax, Chrysomya megacephala, Lucilia cuprina, Dermatobia hominis, and Oestrus ovis). We tested whether 1,287 orthologs have different expression and evolutionary constraints under different scenarios. We found two up-regulated genes; one in species causing cutaneous myiasis that is involved in iron transportation/metabolization (ferritin), and another in species causing traumatic myiasis that responds to reduced oxygen levels (anoxia up-regulated-like). Our evolutionary analysis showed a similar result. In the Ch. hominivorax branch, we found one gene with the same function as ferritin that may be evolving under positive selection, spook. This is the first step towards understanding origins and evolution of parasitic strategy diversity in Oestroidea.

show abstract

“…The situation for invertebrates and especially for insect hosts and their bacterial entomopathogens is less well understood. It is known that insects also possess iron storage proteins [65]–[67] and that entomopathogens synthesize NRPS siderophores [33], [68]. However, whether a competition between host iron sequestration and pathogen iron scavenging similar to the vertebrate system exists and whether insects also use withholding of nutrients (nutritional immunity) as first line defense against invading entomopathogens still remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Production of the Catechol Type Siderophore Bacillibactin by the Honey Bee Pathogen Paenibacillus larvae

et al. 2014

View full text Add to dashboard Cite

The Gram-positive bacterium Paenibacillus larvae is the etiological agent of American Foulbrood. This bacterial infection of honey bee brood is a notifiable epizootic posing a serious threat to global honey bee health because not only individual larvae but also entire colonies succumb to the disease. In the recent past considerable progress has been made in elucidating molecular aspects of host pathogen interactions during pathogenesis of P. larvae infections. Especially the sequencing and annotation of the complete genome of P. larvae was a major step forward and revealed the existence of several giant gene clusters coding for non-ribosomal peptide synthetases which might act as putative virulence factors. We here present the detailed analysis of one of these clusters which we demonstrated to be responsible for the biosynthesis of bacillibactin, a P. larvae siderophore. We first established culture conditions allowing the growth of P. larvae under iron-limited conditions and triggering siderophore production by P. larvae. Using a gene disruption strategy we linked siderophore production to the expression of an uninterrupted bacillibactin gene cluster. In silico analysis predicted the structure of a trimeric trithreonyl lactone (DHB-Gly-Thr)3 similar to the structure of bacillibactin produced by several Bacillus species. Mass spectrometric analysis unambiguously confirmed that the siderophore produced by P. larvae is identical to bacillibactin. Exposure bioassays demonstrated that P. larvae bacillibactin is not required for full virulence of P. larvae in laboratory exposure bioassays. This observation is consistent with results obtained for bacillibactin in other pathogenic bacteria.

show abstract

Iron homeostasis in insects: Insights from Drosophila studies

Cited by 85 publications

References 60 publications

The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster

The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster

Functional genomics of the parasitic habits of blowflies and botflies

Production of the Catechol Type Siderophore Bacillibactin by the Honey Bee Pathogen Paenibacillus larvae

Contact Info

Product

Resources

About