Iron oxide core oil-in-water emulsions as a multifunctional nanoparticle platform for tumor targeting and imaging

Jarzyna, Peter A.; Skajaa, Torjus; Gianella, Anita; Cormode, David P.; Samber, Daniel D.; Dickson, Stephen; Chen, Wei; Griffioen, Arjan W.; Fayad, Zahi A.; Mulder, Willem J. M.

doi:10.1016/j.biomaterials.2009.09.004

Cited by 101 publications

(99 citation statements)

References 31 publications

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“…Oil-in-water NEs were prepared as described previously (42) and presented in Supplementary information. Liposomes were prepared as the NEs, with the following differences: no soybean oil was added, and the sonication time was only 10 min.…”

Section: Synthesis Of Polymeric-and Lipid-based Npsmentioning

confidence: 99%

Labeling nanoparticles: Dye leakage and altered cellular uptake

et al. 2016

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In vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution, cellular uptake and intracellular distribution. Furthermore, NP labeling with trace amounts of dye should not alter NP properties such as interactions with cells or tissues.To allow for versatile NP studies with a variety of fluorescence-based assays, labeling of NPs with different dyes is desirable. Hence, when new dyes are introduced, simple and fast screening methods to assess labeling stability and NP-cell interactions are needed. For this purpose we have used a previously described generic flow cytometry assay; incubation of cells with NPs at 4°C and 37°C. Cell-NP interaction is confirmed by cellular fluorescence after 37°C incubation, and NP dye retention is confirmed when no cellular fluorescence is detected at 4°C.Three different NP-platforms labeled with six different dyes were screened, and a great variability in dye retention was observed. Surprisingly, incorporation of trace amounts of certain dyes was found to reduce, or even inhibit NP uptake. This work highlights the importance of thoroughly evaluating every dye-NP combination before pursuing fluorescent NP applications.3

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Section: Synthesis Of Polymeric-and Lipid-based Npsmentioning

confidence: 99%

Labeling nanoparticles: Dye leakage and altered cellular uptake

et al. 2016

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“…This offers an extremely versatile and tunable compound for a wide variety of applications, ranging from preclinical cellular and molecular imaging studies to drug delivery in patients [72]. For MRI purposes, large quantities of Gd-containing lipids can be incorporated into the nanoparticle’s lipid (bi)layer [73], or alternatively, hydrophobic iron oxide nanocrystals can be encapsulated in the nanoparticle’s core [74,75]. Moreover, additional inclusion of fluorescent moieties [76] or PET/SPECT tracers [77] in the lipid (bi)layer, or hydrophobic particles, such as gold particles [78] and quantum dots [79], in the nanoparticle core, extends the utility of this platform to multimodal imaging set-ups [80].…”

Section: Future Directionsmentioning

confidence: 99%

Imaging Neuroinflammation after Stroke: Current Status of Cellular and Molecular MRI Strategies

et al. 2012

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Cellular and molecular magnetic resonance imaging (MRI) strategies for studying the spatiotemporal profile of neuroinflammatory processes after stroke are increasingly being explored since the first reports appeared about a decade ago. These strategies most often employ (super)paramagnetic contrast agents, such as (ultra)small particles of iron oxide and gadolinium chelates, for MRI-based detection of specific leukocyte populations or molecular inflammatory markers that are involved in the pathophysiology of stroke or plasticity. In this review we describe achievements, limitations and prospects in the field of cellular and molecular MRI of neuroinflammation in preclinical and clinical stroke. Several studies in rodent stroke models have demonstrated the application of MRI contrast agents for imaging of monocyte infiltration, which served as the foundation for pilot (small-scale proof-of-concept) cellular MRI studies in stroke patients. This may be achieved with isolated cells that are loaded with contrast agent through in vitro incubation prior to systemic administration. Alternatively, superparamagnetic iron oxide particles may be directly injected into the circulation to allow in vivo uptake by phagocytic cells. Both strategies have been successfully employed to measure the spatiotemporal profile of invasion of monocytes in and around cerebral ischemic lesions in experimental stroke models. Molecular MRI studies with target-specific contrast agents have shown the capability for in vivo detection of molecular markers after experimental stroke. For example, (super)paramagnetic micro- or nanoparticles that are functionalized with a ligand (e.g. an antibody) for specific cell adhesion molecules, such as E-selectin and vascular cell adhesion molecule 1 (VCAM-1), can target inflamed, activated endothelium, whose presence can subsequently be detected with MRI. Present applications remain limited as most of the currently available contrast agents provide relatively poor contrast enhancement, which is not easily discriminated from endogenous sources of tissue contrast. Nevertheless, current developments of more efficient particles, such as biocompatible liposomes, micelles and nanoemulsions that can contain high payloads of (super)paramagnetic material as well as other substances, such as dyes and drugs, may open a window of opportunities for promising translational multimodal imaging strategies that enable in vivo assessment of (neuroinflammatory) disease markers, therapeutic targets as well as drug delivery after stroke.

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“…In other reports, iron oxide core oil-in-water emulsions or carbon-coated iron nanoparticles were developed as a new multimodality nanoparticle platform for tumor targeting and imaging. 23,24 Additionally, acute toxicity test of CCINs conducted previously confirms that it is safe and nontoxic and meets the basic premise for clinical application. CCINs were characterized by low acute toxicity and mild side effects on the hepatic, renal, and hematological functions within a certain dose range.…”

Section: Dispersion Stability and Thermal Conductivity Of Ccins-nanofmentioning

confidence: 63%

Preparation of carbon-coated iron nanofluid and its application in radiofrequency ablation

Zhang

Chen

et al. 2014

J. Biomed. Mater. Res.

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Carbon-coated iron nanoparticles (Fe@C CCINs) were synthesized by carbon arc discharge method and were studied via X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results showed that CCINs have good core-shell structure and are in size of 40-50 nm. Also, carbon-coated iron nanofluid (CCINs-nanofluid) was prepared via two-step method by dispersing as-prepared CCINs and polyvinylpyrrolidone (PVP) into physiological saline. Its dispersion stability and thermal conductivity were detected by gravity sedimentation method and Hotdisk thermal constant analyzer respectively. The results indicated that CCINs-nanofluid possesses good dispersity and stability. Moreover, CCINs-nanofluid showed enhanced thermal conductivity compared with its base fluid physiological saline. The enhancement of thermal conductivity even reaches 41%. Additionally, CCINs-nanofluid injection aided radiofrequency ablation (RFA) was carried out. The relation between tissue temperature and ablation time revealed that by injecting CCINs-nanofluid into pork livers during RFA, target tissue temperatures were less than 100°C. Dissected pork livers showed that there was little or no tissue charring around the ablation probe. Results of ablation area calculation showed that the ablation area of CCINs-nanofluid injection aided RFA was 67% larger than that of saline injection aided RFA, indicating that a larger-volume tumor tissue necrosis at a single session can be achieved by CCINs-nanofluid injection aided RFA.

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Iron oxide core oil-in-water emulsions as a multifunctional nanoparticle platform for tumor targeting and imaging

Cited by 101 publications

References 31 publications

Labeling nanoparticles: Dye leakage and altered cellular uptake

Labeling nanoparticles: Dye leakage and altered cellular uptake

Imaging Neuroinflammation after Stroke: Current Status of Cellular and Molecular MRI Strategies

Preparation of carbon-coated iron nanofluid and its application in radiofrequency ablation

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