Iron Oxide Nanoparticle–labeled Rat Smooth Muscle Cells: Cardiac MR Imaging for Cell Graft Monitoring and Quantitation

Abstract

Magnetic labeling of smooth muscle cells with anionic maghemite nanoparticles allows detection of cells with MR imaging after local transplantation in the heart.

In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy

“…Furthermore, while the number of intracytoplasmic vesicles containing AMNP decreased as the cells proliferated, the particle number per vesicle increased. These results are in keeping with those obtained with another cell line (34). This technique thus offers optimal conditions for MRI-based in vivo cellular imaging (32).…”

“…We compared the cell amplification factor (Af) with the division of iron mass per cell (DIm). The nanoparticles were equally distributed between daughter cells, as previously described (32,34). However, after 72 hr the DIm was 30% larger than the Af, suggesting On SE and GE images we observed vessels in PD-and T 2 *-weighted sequences, as in control tumors (not shown here).…”

“…After two to three cell divisions, occurred some cells contained large magnetic vesicles (Fig. 3d and e), as previously reported (34). This experiment shows that magnetic endosomes persisted after several cell divisions ( Fig.…”

“…3) indicated that AMNP was exclusively located in endosomes that were dispersed within the cytoplasm. This is compatible with clustered fixation on cationic membrane sites, followed by AMNP internalization along the endocytosis pathway, as described for other cell types (31,34). After 24 hr of culture post-labeling there were fewer magnetic endosomes, but each endosome contained more AMNP than at D0 (Fig.…”

Mixing in a multi-inlet vortex mixer (MIVM) for flash nano-precipitation

“…It is motivated by the use and potential use of nanoparticles in drug delivery, especially poorly water soluble drugs 1-4 , cosmetics [5][6][7] , dyes 3,8 , medical imaging and diagnostic 9,10 , and pesticides 11 . One of the advanced processes to produce nanoparticles is "Flash NanoPrecipitation" 12 , which requires fast mixing of two or more streams to create supersaturation.…”