Iron oxide nanoparticles decrease nuclear fractal dimension of buccal epithelial cells in a time‐dependent manner

Abstract

In this paper, we present results that iron oxide nanoparticles (IONPs) induce time-dependent structural changes in nuclei of buccal epithelial cells. The cells were treated with magnetite, Fe O nanoparticles (spherical shape, diameter 80-100 nanometres). The digital micrographs of the nuclei were made in 3 different time points: 15, 30 and 60 min after the treatment with IONPs, as well as in the control cells. A total of 120 nuclear structures (30 per sample) were analysed. Fractal analysis of nuclei was done in ImageJ software of the National Institutes of Health, (Bethesda, MD, USA). For each nuclear structure, the values of fractal dimension and lacunarity were calculated. There was a time-dependent reduction of nuclear fractal dimension in buccal epithelial cells after exposure to magnetite iron oxide nanoparticles. Negative trend was observed (p < 0.01). Nuclear lacunarity, as another fractal parameter was shown to increase, also in a time-dependent manner, after the treatment with IONPs. To our knowledge, this is the first study to investigate effects of magnetite nanomaterial on nuclear fractal complexity, and also the first to apply fractal analysis method in testing of the interaction between nanoparticles and cell nucleus in this experimental setting.

Peculiarities of Morphodensitometric Indices of Epitheliocytes of the Oral Cavity Mucous Membrane in Children with Latent Iron Deficiency and Mild Iodine Deficiency

“…Significant increase of epitheliocyte area and the decrease of the nuclei area were found. This fact can be explained by the swelling reaction of the cytoplasm to provide more optimal conditions for transmission and synthesis of polypeptides under the conditions of micronutrient deficiency [6]. This proved the presence of gender dimorphism, which was manifested by a greater sensitivity of girls aged 6-11 years to the mild iodine deficiency, latent iron deficiency.…”

“…Iron is the active center of peroxidase involved in the conversion of iodine into the organic form and tyrosine binding to thyroglobulin [4,5]. In iron deficiency intracellular hypoxia occurs, to which the surface epitheliocytes of oral cavity mucous membrane (OCMM) are particularly sensitive [6]. Iodine is an essential element, because it is necessary for the synthesis of thyroid hormones, and is involved in the activation of enhancers of genes that initiate the activity of their expression.…”

Chromatin textural parameters of blood neutrophils are associated with stress levels in patients with recurrent depressive disorder

“…FD was determined on binarized nuclear images, using the FracLac plugin for ImageJ (A. Karperien, Version 2.5, Release 1e, Charles Sturt University, Australia) [21]. During the analysis, a special box-counting method is performed, the structure is covered by a number of boxes (N) on different scales (ε), after which FracLac forms a logarithmic graph (Figure 3) and calculates FD from the slope of the regression line: FD = regression slope [ln(N)/ ln (ε)] FD was previously used on numerous occasions in cell biology in order to detect small structural alterations [16,22]. Although not directly related to ASM or IDM, it may provide additional information on the nature and causes of their potential change.…”

Bile lipids in rats under chronic social stress

“…Зовсім незначна кількість праць стосується отримання даних, які базуються на доказових морфометричних методах, що дозволяє дати об'єктивну оцінку стану букальних епітеліоцитів у різноманітній патологічній і, що важливіше, на нашу думку, передпатологічній ситуації. Зокрема, останніми роками окремі автори провели подібні спроби під час визначення площі ядер епітеліоцитів і подали об'єктивну оцінку цього показника за різних станів організму (Moscicka-Studzinska et al, 2009;Nikolovski et al, 2017).…”

“…У процесі морфологічного дослідження виявлено порушення форми клітин букального епітелію та їх ядер, що характерно для першої стадії апоптозу (Nikolovski et al, 2017). Із даних літератури (Mishra et al, 2017) відомо, що зміна форми клітини та її ядра, а також наявність ацидофільних гранул свідчать про апоптоз, тому що за некрозу у клітинних мембранах (зокрема, ядра та клітинній оболонці) відбувається деструктивні зміни іншого характеру.…”