Iron Oxide Nanoparticles (IONPs) with potential applications in plasmid DNA isolation

Sosa-Acosta, J.R; Silva, J.A.; Fernández‐Izquierdo, Leunam; Díaz-Castañón, S.; Ortiz, M.; Zuaznabar‐Gardona, Julio C.; Díaz-García, Alicia

doi:10.1016/j.colsurfa.2018.02.062

Cited by 43 publications

(26 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The XRD pattern obtained for the MNPs is presented in Figure (c). As shown in the figure, the XRD spectrum of the MNPs exhibited the diffraction peaks at 2θ = 30.1°, 35.4°, 43.0°, 53.2°, 57.0°, and 62.0° corresponding to the crystal planes of Fe 3 O 4 with a spinal structure (220), (311), (400), (422), (511), and (440), respectively . Also, the absence of extraneous peaks in the diffraction pattern confirms the purity of the synthesized MNPs.…”

Section: Resultsmentioning

confidence: 59%

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

Lalegül‐Ülker

Vurat

Elçin

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

Magnetically responsive polymer composites have great potential for use in diverse biomedical applications. In this study, composite biomaterials consisting of silk fibroin (SF) and superparamagnetic iron oxide nanoparticles (SPIONs) were fabricated by the electrospinning method. Two different methods were employed to incorporate the SPIONs into the SF nanofibers. In the first encapsulation method (M1), SPIONs (1.0, 3.0, and 5.0 wt%) were initially included in the electrospinning solution. In the second dip-coating method (M2), electrospun SF nanofiber mats were immersed in the aqueous suspensions of SPIONs (10, 30 and 50% v/v). Then, the pure and composite silk fibroin composite mats were comparatively evaluated for their morphological, chemical, magnetic, mechanical and in vitro biological properties, by using a number of methods including SEM, TEM, FTIR, XRD, EDS, VSM, TGA, mechanical tensile tests, as well as by indirect in vitro cytotoxicity and in vitro hemocompatibility analyses. Overall findings suggested that, while M1 nanofiber mats could be a suitable candidate for use in tissue engineering as a magnetically responsive cytocompatible scaffold, the M2 nanofiber mats perhaps could be more appropriate as an interface for triggering the in vitro stem cell differentiation and/or biosensor applications.

show abstract

Section: Resultsmentioning

confidence: 59%

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

Lalegül‐Ülker

Vurat

Elçin

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…The extraction of genomic DNA with high quality is a critical step for molecular studies. 10 In this regard, different methods can be performed, including phenol-chloroform and DNA extraction kits. However, more appropriate methods are needed due to the use of toxic substances, especially phenol and the high cost of commercial kits.…”

Section: Discussionmentioning

confidence: 99%

“…9 DNA extraction is a major step in molecular studies on pathogenic bacteria. 10 Staphylococcus aureus is one of the most important pathogens that cause extremely important infections. 11 In recent years, the important role of S. aureus in causing nosocomial and community infections has led to increased molecular research on this bacterium.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles

Rostami

Firoozeh

Zibaei

et al. 2020

Int J Enteric Pathog

View full text Add to dashboard Cite

Background: There are different methods for genomic DNA extraction. Magnetic nanoparticles (MNPs) exhibit many important features making them suitable for DNA extraction. Objectives: The aim of this study was to compare the effect of monolayer and bilayer MNPs on genomic DNA extraction. Materials and Methods: The genomic DNA was extracted from the Staphylococcus aureus strain ATCC 25923 and clinical isolates using monolayer MNPs SiO2 and Fe3O4 and bilayer MNPs SiO2/Fe3O4. Then, the quality and quantity of the obtained genomic DNA were compared with both NPs. Results: The obtained results showed the concentration and purity of the extracted genomic DNA using bilayer magnetic NPs was significantly higher in comparison to the extracted genomic DNA by monolayer MNPs. Conclusion: In general, surface-coated MNPs are much more efficient than naked MNPs for genomic DNA extraction.

show abstract

“…The presence of charged groups may be responsible not only for the stability and the agglomeration of the nanoparticles in suspension, but also for the interaction strength particularly when electrostatic interactions play the primary role. Accordingly, highly negative charged molecules, such as DNA, can be repulsed at high pH [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Bio-nano interactions: binding proteins, polysaccharides, lipids and nucleic acids onto magnetic nanoparticles

2021

View full text Add to dashboard Cite

The major interest in nanoparticles as an application platform for biotechnology arises from their high surface-to-volume ratio. Iron oxide nanoparticles (IONPs) are particularly appealing due to their superparamagnetic behavior, which enables bioseparation using external magnetic fields. In order to design advanced biomaterials, improve binding capacities and develop innovative processing solutions, a thorough understanding of the factors governing organic-inorganic binding in solution is critical but has not yet been achieved, given the wide variety of chemical and physical influences. This paper offers a critical review of experimental studies of the interactions between low cost IONPs (bare iron oxides, silica-coated or easily-functionalized surfaces) and the main groups of biomolecules: proteins, lipids, nucleic acids and carbohydrates. Special attention is devoted to the driving forces and interdependencies responsible of interactions at the solid-liquid interface, to the unique structural characteristics of each biomolecular class, and to environmental conditions influencing adsorption. Furthermore, studies focusing on mixtures, which are still rare, but absolutely necessary to understand the biocorona, are also included. This review concludes with a discussion of future work needed to fill the gaps in knowledge of bio-nano interactions, seeking to improve nanoparticles’ targeting capabilities in complex systems, and to open the door for multipurpose recognition and bioseparation processes.

show abstract

Iron Oxide Nanoparticles (IONPs) with potential applications in plasmid DNA isolation

Cited by 43 publications

References 45 publications

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles

Bio-nano interactions: binding proteins, polysaccharides, lipids and nucleic acids onto magnetic nanoparticles

Contact Info

Product

Resources

About