Irradiation pretreatment enhances the therapeutic efficacy of platelet-membrane-camouflaged antitumor nanoparticles

Abstract

Background: Cell membrane-based nanocarriers are promising candidates for delivering antitumor agents. The employment of a simple and feasible method to improve the tumor-targeting abilities of these systems is appealing for further application. Herein, we prepared a platelet membrane (PM)-camouflaged antitumor nanoparticle. The effects of irradiation pretreatment on tumor targeting of the nanomaterial and on its antitumor action were evaluated. Results: The biomimetic nanomaterial constructed by indocyanine green, poly(d,l-lactide-co-glycolide), and PM is termed PINPs@PM. A 4-Gy X-ray irradiation increased the proportions of G2/M phase and Caveolin-1 content in 4T1 breast cancer cells, contributing to an endocytic enhancement of PINPs@PM. PINPs@PM produced hyperthermia and reactive oxygen species upon excitation by near-infrared irradiation, which were detrimental to the cytoplasmic lysosome and resulted in cell death. Irradiation pretreatment thus strengthened the antitumor activity of PINPs@PM in vitro. Mice experiments revealed that irradiation enhanced the tumor targeting capability of PINPs@PM in vivo. When the same dose of PINPs@PM was intravenously administered, irradiated mice had a better outcome than did mice without X-ray pretreatment. Conclusion: The study demonstrates an effective strategy combining irradiation pretreatment and PM camouflage to deliver antitumor nanoparticles, which may be instrumental for targeted tumor therapy.

Recent Advancements in Serum Albumin-Based Nanovehicles Toward Potential Cancer Diagnosis and Therapy

“…Although multitudinous NIR-light-absorbing agents have been validated to be available for cancer therapy and show encouraging therapeutic effects, they still possess some drawbacks. Fox example, an NIR cyanine dye, indocyanine green (ICG), which has been approved by the FDA, is unstable in aqueous solutions and easily self-bleach and is rapidly eliminated from the body, leading to their poor photostability and unfavorable antitumor effect ( Ma et al, 2013 ; Shen et al, 2019 ; Chen et al, 2020 ). To cope with these issues, various nanocarriers, such as liposomes ( Lajunen et al, 2016 ), micelles ( Kirchherr et al, 2009 ), and vesicles ( Zhu et al, 2015 ), have been developed to effectively deliver ICG for improving stability and tumor targeting.…”

Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection

“…The flow cytometry was performed as we recently discribed. 13 Briefly, HK-2 was seeded in a 6-well plate at a density of 2×10 5 CFU/well and cultured overnight in DMEM containing 10%…”

“…Taking the advantage of functional elements on human platelet membranes, we have developed some CMBNPs with the capabilities of tumor targeting and immune escape. [12][13] In this study, we selected the membrane of human embryonic kidney-239T cells highly expressing human ACE2 (HEK-293T-hACE2) to prepare CMBNPs after analyzing the ACE2 content in five human cells ( Figure 1). The antiviral activity and mechanism of action of this nanomaterial were investigated by pseudovirus neutralization assay, spike adhesion experiment, and proteomics analysis.…”

Radiodynamic Therapy Using TAT Peptide-Targeted Verteporfin-Encapsulated PLGA Nanoparticles

“…In addition, the fact that the DNA double-strand breaks increased compared to the sole X-ray control, even without the TAT, suggests that other effects may be occurring. For example, X-irradiation is known to increase cellular and nuclear membrane permeability [61,62] such that it could allow a degree of nuclear internalization even without the TAT. The greater DNA damage with the TAT NPS may then reflect simply a greater total-cell uptake rather than a specific nuclear localization.…”