Irreversible corneal decompensation in patients treated with topical dorzolamide

konowal, Alexandra; Morrison, John C.; Brown, Steven V.L.; Cooke, Daniel L; Maguire, Leo J.; Verdier, de; Fraunfelder, F. T.; Dennis, Richard F.; Epstein, Randy J.

doi:10.1016/s0002-9394(98)00438-3

Cited by 196 publications

(95 citation statements)

References 13 publications

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“…One study demonstrated corneal decompensation in patients treated with topical dorzolamide (Konowal et al 1996), however, all patients had compromised corneal endothelium and one would expect that the alternative pathways might not be sufficient to maintain the fluid transport.…”

Section: Discussionmentioning

confidence: 99%

Influence of dorzolamide on corneal thickness, endothelial cell count and corneal sensibility

Kaminski¹,

Hommer²,

Koyuncu³

et al. 1998

Acta Ophthalmologica Scandinavica

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Section: Discussionmentioning

confidence: 99%

Influence of dorzolamide on corneal thickness, endothelial cell count and corneal sensibility

Kaminski¹,

Hommer²,

Koyuncu³

et al. 1998

Acta Ophthalmologica Scandinavica

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“…Although carbonic anhydrase enzyme is expressed by the corneal endothelium and is associated with endothelial pump function, the effect of CAIs on the graft is likely to be clinically significant only if the graft is near failure. 37,38 Surgical management of post-keratoplasty glaucoma…”

Section: Medical Management Of Post-keratoplasty Glaucomamentioning

confidence: 99%

Management of patients with combined glaucoma and corneal transplant surgery

Banitt

Lee

2009

Eye

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Penetrating keratoplasty (PKP) is associated with an increased risk of secondary glaucoma. The development of glaucoma after PKP is an important risk factor for decreased corneal graft survival. The incidence of glaucoma after corneal transplant as well as the mechanism of developing increased intraocular pressure is reviewed in this paper. Treatments for post-PKP glaucoma include medications, laser, and surgery. The most frequent surgical glaucoma intervention is implantation of a glaucomadrainage device. Recent advances in corneal transplantation surgery may help to decrease corneal failure and the risk of developing post-keratoplasty glaucoma.

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“…On the other hand, when endothelial cell loss is accelerated, (e.g. Fuchs' Endothelial Dystrophy, trauma, chronic inflammation), corneal hydration will increase and sensitivity to topical CAIs can be uncovered (Konowal et al, 1999). These findings indicate that human corneal endothelium is likely to possess similar transport function as shown in animal studies.…”

Section: Introductionmentioning

confidence: 84%

Identification and cloning of the Na/HCO3− cotransporter (NBC) in human corneal endothelium

Sun

Bonanno

2003

Experimental Eye Research

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Fluid secretion by the corneal endothelium is associated with the net flux of from basolateral (stromal) to apical (anterior chamber) sides of the tissue. In this study we asked if cotransporter (NBC-1) protein expression and functional activity are present in freshly isolated human corneal endothelium. Immunoblot analysis using a polyclonal antibody to NBC-1 showed a single band at ~130 kDa. Indirect immunofluorescence indicated that NBC-1 is expressed on the basolateral, but not apical side of human corneal endothelium. RT-PCR was used to determine whether the kidney or pancreatic isoform of NBC-1 is expressed. Using the specific primers for pNBC and kNBC isoforms, RT-PCR showed that only pNBC could be detected in human corneal endothelium. The product was cloned and confirmed by sequencing. Full-length NBC-1 was also cloned from human corneal endothelium. This clone (hcNBC) is 100% identical to the longer, more common form of NBC [pNBC; 1079 amino acids (aa); 122 kDa in human heart, pancreas and prostate]. To test for functional activity of NBC-1, freshly isolated endothelium was loaded with the pH sensitive fluorescent dye BCECF and fluxes were measured. fluxes were Na + -dependent, electrogenic and H 2 -DIDS sensitive. We conclude that the long isoform of the sodium bicarbonate cotransporter (pNBC-1) is expressed on the basolateral side of fresh human corneal endothelium (hcNBC). The shorter form, kNBC, could not be detected. As in bovine corneal endothelium, hcNBC is instrumental in loading into endothelial cells from the basolateral membrane.

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Irreversible corneal decompensation in patients treated with topical dorzolamide

Cited by 196 publications

References 13 publications

Influence of dorzolamide on corneal thickness, endothelial cell count and corneal sensibility

Influence of dorzolamide on corneal thickness, endothelial cell count and corneal sensibility

Management of patients with combined glaucoma and corneal transplant surgery

Identification and cloning of the Na/HCO3− cotransporter (NBC) in human corneal endothelium

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