Is 24-Color FISH Detection of In-Vitro Radiation-Induced Chromosomal Aberrations Suited to Determine Individual Intrinsic Radiosensitivity?

Kuechler, Alma; Neubauer, Susann; Grabenbauer, Gerhard G.; Claussen, Uwe; Liehr, Thomas; Sauer, Rolf; Wendt, Thomas

doi:10.1007/s00066-002-0904-0

Cited by 25 publications

(23 citation statements)

References 26 publications

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“…After an orienting classification of chromosomal rearrangements by 24-color FISH as described by Kuechler et al (2002), MCB was performed on all chromosomes involved in rearrangements in HT-29 clone 19A (Fig. 1).…”

mentioning

confidence: 99%

Precise breakpoint characterization of the colon adenocarcinoma cell line HT‐29 clone 19A by means of 24‐color fluorescence in situ hybridization and multicolor banding

Kuechler

Weise

Michel

et al. 2002

Genes Chromosomes & Cancer

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confidence: 99%

Precise breakpoint characterization of the colon adenocarcinoma cell line HT‐29 clone 19A by means of 24‐color fluorescence in situ hybridization and multicolor banding

Kuechler

Weise

Michel

et al. 2002

Genes Chromosomes & Cancer

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“…In contrast, a 24-color FISH detected the same frequency of radiation-induced aberrations in two lymphoblastoid cell lines derived from NBS heterozygotes and in a control cell line [Kuechler et al, 2002]. Likewise, the frequency of chromosomal aberrations in G2-irradiated lymphoblastoid cells from seven out of eight NBS heterozygotes was similar to that of control cell lines [Tanzanella et al, 2003].…”

Section: Introductionmentioning

confidence: 47%

“…Except for a few studies reporting somewhat controversial data [Pincheira et al, 1998;Kuechler et al, 2002;Neubauer et al, 2002;Tanzanella et al, 2003], little is known about the radiosensitivity of heterozygous NBS1 carriers at the cellular level. Cells from the parents of NBS patients have normal levels of chromosomal damage in G2, and the duration of the G2 phase of the cell cycle in X-irradiated lymphocytes from NBS parents is similar to control cells [Pincheira et al, 1998].…”

Section: Introductionmentioning

confidence: 97%

Radiation‐induced DNA damage and repair in peripheral blood mononuclear cells from Nijmegen breakage syndrome patients and carriers assessed by the Comet assay

Bürger

Schindler

Fehn

et al. 2006

Environ and Mol Mutagen

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Nijmegen breakage syndrome (NBS) patients and carriers are predisposed to malignancy and are often treated with X-irradiation. In the present study, the single-cell gel electrophoresis (Comet) assay was used to examine radiation-induced DNA damage and repair in peripheral blood mononuclear cells from NBS patients (n=13) and carriers (n=36) of six unrelated families. Cells from apparently healthy donors (n=10) and from breast cancer patients with normal clinical radiosensitivity (n=10) served as controls. Cells were irradiated with 5 Gy of X-rays and assayed for initial DNA damage and for residual DNA damage after 40 min of repair; the kinetics of DNA repair also was estimated. In addition, the nuclear area of unirradiated cells was extracted from the Comet data. The initial radiation-induced DNA fragmentation indicated that cells from members of two out of six NBS families were significantly more sensitive to X-irradiation than cells from the controls. Cells from four NBS families had longer DNA repair half-time values, while cells from five NBS families had significantly increased residual DNA damage following repair. The mean nuclear area of unirradiated cells processed in the Comet assay was 1.3-fold higher in cells from all NBS families than in the controls (P<0.05). Notably, the Comet assay parameters (initial and residual DNA damage and the repair kinetics) of irradiated NBS cells predicted the carrier status of the majority (86%) of blindly tested individuals. The prediction of NBS status was higher if the nuclear area of unirradiated cells was used as the endpoint. The results of this study suggest that the impaired radiation response of NBS cells should be taken into account if radiotherapy of NBS patients and carriers is required.

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“…Despite intensive research [1,5,17,21,35] on individual radiosensitivity, there is still no reliable test available which can be employed predictively in clinical everyday use to describe the individual degree of radiosensitivity. On the cellular level, ionizing radiation produces chromosomal aberrations, which can be used as indicators for radiosensitivity [15,35].…”

Section: Introductionmentioning

confidence: 99%

Impact of Various Parameters in Detecting Chromosomal Aberrations by FISH to Describe Radiosensitivity

Keller¹,

Kuechler

Liehr

et al. 2004

Strahlenther Onkol

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Is 24-Color FISH Detection of In-Vitro Radiation-Induced Chromosomal Aberrations Suited to Determine Individual Intrinsic Radiosensitivity?

Cited by 25 publications

References 26 publications

Precise breakpoint characterization of the colon adenocarcinoma cell line HT‐29 clone 19A by means of 24‐color fluorescence in situ hybridization and multicolor banding

Precise breakpoint characterization of the colon adenocarcinoma cell line HT‐29 clone 19A by means of 24‐color fluorescence in situ hybridization and multicolor banding

Radiation‐induced DNA damage and repair in peripheral blood mononuclear cells from Nijmegen breakage syndrome patients and carriers assessed by the Comet assay

Impact of Various Parameters in Detecting Chromosomal Aberrations by FISH to Describe Radiosensitivity

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