Is auxin enough? Cytokinins and margin patterning in simple leaves

Navarro-Cartagena, Sergio; Micol, José Luis

doi:10.1016/j.tplants.2022.08.019

Cited by 8 publications

(4 citation statements)

References 174 publications

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“…Notably, similar to CsLMI1 , CsDRNL is also implicated in controlling the development of serrated leaves in Citrus . This suggests the existence of similar regulatory mechanisms involving cell proliferation or expansion in both oil gland formation and leaf serration production, and the latter process is known to be regulated by the interplay of auxin and cytokinin ( 34 ). Furthermore, DRNL ’s homolog is indispensable in leaf initiation and promotes the development of peltate trichomes in tomatoes, which are multicellular secretory structures on the surface of the epidermis ( 10 , 35 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular regulation of oil gland development and biosynthesis of essential oils in Citrus spp.

Wang,

Ren,

Zhou

et al. 2024

Science

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Secretory structures in terrestrial plants serve as reservoirs for a variety of secondary metabolites. Among these, the secretory cavity of the Rutaceae family is notable for containing essential oils with a wide range of applications. However, the molecular basis underlying secretory cavity development is unknown. Here, we reveal a molecular framework for Citrus oil gland formation. Using genetic mapping and genome editing, we demonstrated that this process requires LATE MERISTEM IDENTITY1 (LMI1), a key regulator of leaf serration. A conserved GCC box element of the LMI1 promoter recruits DORNROSCHEN-like (DRNL) for transcriptional activation. This DRNL-LMI1 cascade triggers MYC5 activation, facilitating the development of oil glands and the biosynthesis of essential oils. Our findings spotlight cis -regulatory divergence within leaf shape genes, propelling novel functional tissue formation.

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Section: Discussionmentioning

confidence: 99%

Molecular regulation of oil gland development and biosynthesis of essential oils in Citrus spp.

Wang,

Ren,

Zhou

et al. 2024

Science

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“…Some mesophyll cells adjacent to this tissue also replicate DNA but rarely divide, resulting in cell growth by endoreduplication. In A. thaliana , a complex regulation of transcription factors contributes to leaf margin growth, although the molecular mechanism remains to be elucidated [ 30 , 31 ]. We speculate that exogenous ZT could activate the marginal meristem of tomato cotyledons, probably through the WUSCHEL-RELATED HOMEOBOX1 (WOX1) and WOX3 transcription factors, which in A. thaliana are known to positively regulate the expression of YUCCA1 ( YUC1 ) and YUC4 to synthesize auxin in the margin and thus control lamina outgrowth [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants

Yaroshko

Pasternak

Larriba

et al. 2023

Plants

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Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the effect of different concentrations of zeatin and indole-3-acetic acid on the regeneration of cotyledon explants in tomato cultivars M82 and Micro-Tom. We evaluated regeneration parameters such as the percentage of callus formation and the area of callus formed, as well as the initiation percentage and the number of adventitious shoots. The best hormone combination produced shoot-like structures after 2–3 weeks. We observed the formation of leaf primordia from these structures after about 3–4 weeks. Upon transferring the regenerating micro-stems to a defined growth medium, it was possible to obtain whole plantlets between 4 and 6 weeks. This hormone combination was applied to other genotypes of S. lycopersicum, including commercial varieties and ancestral tomato varieties. Our method is suitable for obtaining many plantlets of different tomato genotypes from cotyledon explants in a very short time, with direct applications for plant transformation, use of gene editing techniques, and vegetative propagation of elite cultivars.

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“…Leaf development in plants is regulated by various phytohormones, including auxin and cytokinin ( Navarro-Cartagena and Micol, 2023 ). The morphology of leaves varies across different plant species, primarily due to the distinct arrangement of leaf lobes or teeth in single leaves and leaflets in compound leaves ( Hay and Tsiantis, 2006 ; Runions et al., 2017 ; Kierzkowski et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding and comparative RNA-Seq analysis provide new insights into leaf formation using a novel resource of high-yielding Epimedium koreanum

Yang,

Fan,

Guo

et al. 2023

Front. Plant Sci.

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Epimedium koreanum Nakai, a well-known traditional Chinese medicinal herb, has been widely used to treat osteoporosis and sexual dysfunction for thousands of years. However, due to the decreasing population of East Asian natural resources, yearly output of Epimedium crude herb has been in low supply year by year. In this study, an unusual variety of E. koreanum was discovered in Dunhua, Jilin Province, the northernmost area where this variety was found containing 6 individuals, with three branches that had 27 leaflets, which is much more than the typical leaflet number of 9. Firstly, the novel E. koreanum varety was identified using DNA barcodes. Then, 1171 differentially expressed genes (DEGs) were discovered through parallel RNA-seq analysis between the newly discovered variety and wild type (WT) E. koreanum plant. Furthermore, the results of bioinformatics investigation revealed that 914 positively and 619 negatively correlated genes associated with the number of leaflets. Additionally, based on RNA-Seq and qRT-PCR analysis, two homologous hub TCP genes, which were commonly implicated in plant leaf development, and shown to be up regulated and down regulated in the discovered newly variety, respectively. Thus, our study discovered a novel wild resource for leaf yield rewarding medicinal Epimedium plant breeding, provided insights into the relationship between plant compound leaf formation and gene expression of TCPs transcription factors and other gene candidates, providing bases for creating high yield cultivated Epimedium variety by using further molecular selection and breeding techniques in the future.

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Is auxin enough? Cytokinins and margin patterning in simple leaves

Cited by 8 publications

References 174 publications

Molecular regulation of oil gland development and biosynthesis of essential oils in Citrus spp.

Molecular regulation of oil gland development and biosynthesis of essential oils in Citrus spp.

Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants

DNA barcoding and comparative RNA-Seq analysis provide new insights into leaf formation using a novel resource of high-yielding Epimedium koreanum

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