Is Color‐Specific Photoswitching in Dual‐Color Fluorescence Systems Possible? Manipulating Intermolecular Energy Transfer among Two Different Fluorophores and One Photoswitch

Kim, Dojin; Kwon, Ji Eon; Park, Soo Young

doi:10.1002/adom.201500699

Cited by 31 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A large number of fluorescence-color phototunable DAE-based NPs have been developed by incorporating two or more energy level-matched fluorescent dyes into NPs together with the DAE unit and controlling the fluorescence intensity of each component with a pcFRET mechanism. For example, Park and coworkers 92,93 color dramatically changed from green to blue. This fluorescence color change is attributed to the efficient energy transfer from only the BTD unit to the closed-ring isomer of the DAE unit in the P-dots because of the much larger spectral overlap between the fluorescence band of the BTD unit and the absorption band of the DAE unit compared to the case between the phenylene unit and the DAE closed-ring isomer unit.…”

Section: Fluorescence Color Modulationmentioning

confidence: 99%

Photochromic fluorophores at the molecular and nanoparticle levels: fundamentals and applications of diarylethenes

2018

View full text Add to dashboard Cite

Fluorescent molecules and materials are widely used in many areas in physics, chemistry, and biology as emitters, tags, or sensors. The possibility of controlling their fluorescence signal by light, namely, fluorescence photoswitching, down to the nanoscale level can then dramatically extend their fields of applications. This review focuses on fluorescent and photochromic diarylethene-based nanosystems. The choice of the diarylethene family has been driven by its excellent photoswitching properties (conversion yield, bistability, fatigue resistance), which make them fully appropriate when high-performance behavior is required. The different molecular and nanomaterial designs providing suitable combinations of fluorescence and photochromism are summarized. Besides the inherently fluorescent diarylethene molecules, chemical association between photochromic and fluorescent molecular units can advantageously lead to fluorescence photoswitching thanks to resonance energy transfer or intramolecular electron transfer processes. Furthermore, the preparation of nanoscale emissive materials involving diarylethene units paves the way to new interesting features, such as near-infrared control of emissive and photoswitchable nanohybrids, giant amplification of the fluorescence photoswitching in organic nanoparticles, or fluorescence color modulation. Many applications derived from such fluorescent diarylethene-based molecules and nanomaterials have been developed recently, especially in the field of biology for fluorescence biolabeling and super-resolution imaging but also for photocontrol of biological functions. Extremely promising prospects are expected in the near future.

show abstract

Section: Fluorescence Color Modulationmentioning

confidence: 99%

Photochromic fluorophores at the molecular and nanoparticle levels: fundamentals and applications of diarylethenes

2018

View full text Add to dashboard Cite

show abstract

“…[19] Diarylethenes represent ap owerful class of organic photoswitches that have various applications such as data storage and chemical sensing. [20][21][22] Despite their wide variability,t he lack of derivatives in which the closed form has astrong fluorescence in the visible range has prevented their direct application in super-resolution imaging. Strategies to covalently link diarylethene switches with suitable fluorophores and to utilize the energy [23] or electron transfer [24] of the resulting multichromophoric systems have been successful, but require extended syntheses and result in rather large probes.L abeling structures with such extended probes can significantly alter the structures to be visualized.…”

mentioning

confidence: 99%

Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

et al. 2016

View full text Add to dashboard Cite

The in situ imaging of soft matter is of paramount importance for a detailed understanding of functionality on the nanoscopic scale. Although super-resolution fluorescence microscopy methods with their unprecedented imaging capabilities have revolutionized research in the life sciences, this potential has been far less exploited in materials science. One of the main obstacles for a more universal application of superresolved fluorescence microscopy methods is the limitation of readily available suitable dyes to overcome the diffraction limit. Here, we report a novel diarylethene-based photoswitch with a highly fluorescent closed and a nonfluorescent open form. Its photophysical properties, switching behavior, and high photostability make the dye an ideal candidate for photoactivation localization microscopy (PALM). It is capable of resolving apolar structures with an accuracy far beyond the diffraction limit of optical light in cylindrical micelles formed by amphiphilic block copolymers.The nanoscopic structure of soft-matter materials determines their properties. [1] Therefore, methods to directly visualize structures in the nanometer range are of paramount importance for the ongoing evolution of novel materials with specialized and adaptive properties for sophisticated applications. Scanning probe microscopy techniques give access to the nanometer range and determine surface properties such as topology and softness, [2,3] while modern electron microscopy methods, such as scanning electron microscopy (SEM) [4,5] and transmission electron microscopy (TEM), [6][7][8] can yield structural information even in the subnanometer range when there is sufficient electron density contrast. Despite the success of these methods, they are technically demanding and time-consuming. Furthermore, many softmatter samples possess poor electron contrast, and require non-invasive in situ imaging below the surface as well as the possibility to directly study dynamics. In recent years, superresolved fluorescence microscopy has revolutionized optical imaging, [9][10][11][12][13][14] by utilizing the photophysical or photochemical switching of fluorescent dyes in a sophisticated manner in combination with modern optics. So far, the life sciences have benefited, in particular, from the new possibilities of resolving structures well beyond the diffraction limit of light. Only a few examples of the application of super-resolution microscopy to materials science have been reported, [15][16][17][18] since concepts that require, for example, the addition of (polar) switching buffers often fail for these systems. Therefore, the main bottleneck for more universal applications of super-resolution imaging are switchable dyes with suitable (photo-)physical and chemical properties, such as high photostability, adjustable switching rates, minimum interaction with the environment to be probed, and simple design, with the possibility of multiple and straightforward derivatization for the specific labeling of structures or compartments. [19] ...

show abstract

“…Upon photoswitching of a diarylethene, only the green emission could be turned on/off selectively, but with a limited efficiency of 54%. Recently, we demonstrated color-specific photoswitching by employing the excited-state intramolecular proton transfer (ESIPT) process in an all-organic system, which consists of two ESIPT dyes with different emission colors (blue and orange) and a diarylethene 46 . As the energy transfer between the ESIPT dyes was blocked according to the proton transfer morphing 47 , the blue emission could be selectively switched (59%) upon photochromic switching.…”

Section: Introductionmentioning

confidence: 99%

Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy

et al. 2019

Self Cite

View full text Add to dashboard Cite

Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.

show abstract

Is Color‐Specific Photoswitching in Dual‐Color Fluorescence Systems Possible? Manipulating Intermolecular Energy Transfer among Two Different Fluorophores and One Photoswitch

Cited by 31 publications

References 54 publications

Photochromic fluorophores at the molecular and nanoparticle levels: fundamentals and applications of diarylethenes

Photochromic fluorophores at the molecular and nanoparticle levels: fundamentals and applications of diarylethenes

Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy

Contact Info

Product

Resources

About