Is exposure to environmental or industrial endocrine disrupting estrogen-like chemicals able to cause genomic instability

Roy, Deodutta; Colerangle, John B.; Singh, Kamleshwar Prasad

doi:10.2741/a332

Cited by 49 publications

(29 citation statements)

References 56 publications

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“…When absorbed into the body, EDCs either mimic or block hormones and disrupt the body's normal functions. Consequences of exposure can include reproductive impairment (Vos et al 2000), DNA damage, and genomic instability (Roy et al 1998). Among 48 EDCs evaluated by Choi et al (2004), estrogenic effects were the most predominant in pesticides, while effects on thyroid hormone were found for heavy metals.…”

Section: Endocrine Disruptors and Estrogenic Chemicalsmentioning

confidence: 99%

“…In 2002, the World Health Organization defined EDCs as "an exogenous substance or mixture that alters functions of the endocrine system and consequently causes adverse health effects in an intact organism, or its progeny, or (sub)populations" (World Health Organization 2002). The commonly recognized EDCs come from many different types of chemicals but include persistent organic pollutants such as DDT and analogs; industrial compounds such as dioxins, bisphenol A, and polychlorinated biphenyls (PCBs); pesticides including chlorinated insecticides, imidazoles, and triazines; chemical substances that are widely used in cosmetics such as phthalates and parabens; and several heavy metals (Roy et al 1998;Oishi 2002;Choi et al 2004;Carr and Norris 2006). The list of metallic EDCs includes cadmium, mercury, arsenic, lead, manganese, and zinc (Iavicoli et al 2009).…”

Section: Endocrine Disruptors and Estrogenic Chemicalsmentioning

confidence: 99%

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Infectious Disease and Contaminants in Urban Wildlife: Unseen and Often Overlooked Threats

2014

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Section: Endocrine Disruptors and Estrogenic Chemicalsmentioning

confidence: 99%

Section: Endocrine Disruptors and Estrogenic Chemicalsmentioning

confidence: 99%

Infectious Disease and Contaminants in Urban Wildlife: Unseen and Often Overlooked Threats

2014

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“…Dis Aquat Org 65: [187][188][189][190][191][192][193][194][195] 2005 can neoplasia has been motivated by the question as to whether bivalves can be used as bioindicators in environmental monitoring (Roy et al 1998, Brousseau et al 2000, Fournier et al 2001, Sauve et al 2002.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…Environmental carcinogens such as hydrocarbons (Yevich & Barszcz 1977, Naes et al 1995, herbicides (van Beneden et al 1993), biotoxins (Landsberg 1996, Roy et al 1998) and infectious agents (Farley et al 1972, Ford et al 1997 have been suggested as the main risk factors for disseminated neoplasia. Research on mollus-can neoplasia has been motivated by the question as to whether bivalves can be used as bioindicators in environmental monitoring (Roy et al 1998, Brousseau et al 2000, Fournier et al 2001, Sauve et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis

Smolarz¹,

Renault²,

Soletchnik³

et al. 2005

Dis. Aquat. Org.

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A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major population of cells in peak B' and more than 10% of cells in the C' peak. Stage 2 consisted of a lower percentage of cells in peak B' and more than 25% of cells in peak C'. Stage 3 of the neoplasia was characterised by a further reduction in peak B' and more than 40% of cells in peak C'. Flow cytometry allowed for objective detection of neoplasia and provided a rapid method for measuring the DNA content of thousands of cells per individual. The accuracy of flow cytometry was assessed by comparing with standard histological techniques, used here as a reference technique for the detection of neoplasia, and with chromosome analysis. All individuals were analysed in parallel using the 3 techniques. The proportion of normal and affected individuals diagnosed using flow cytometry was comparable to the proportion determined by histology and chromosome analysis. KEY WORDS: Flow cytometry · Histology · Cytogenetics · Neoplasia · Macoma balthica · Baltic Sea Resale or republication not permitted without written consent of the publisherDis Aquat Org 65: [187][188][189][190][191][192][193][194][195] 2005 can neoplasia has been motivated by the question as to whether bivalves can be used as bioindicators in environmental monitoring (Roy et al. 1998, Brousseau et al. 2000, Fournier et al. 2001, Sauve et al. 2002.High prevalence of neoplasia can be found in Macoma balthica L. sampled from industrialised waterways around the Gulf of Gdansk in Poland (Thiriot-Quiévreux & Wolowicz 1996). Clams at these sites are known to be exposed to a range of chemical carcinogens and pollutants (Wolowicz 1994, Szefer et al. 1996, Sokolowski et al. 1999, Hummel et al. 2000. Neoplasia in M. balthica have also been reported from the Chesapeake Bay (Christensen et al. 1974) and Finnish coast (Pekkarinen 1993).This report describes a flow cytometry protocol for the detection of gill neoplasia in Macoma balthica from the Gulf of Gdansk (Baltic Sea, Poland). Flow cytometry has already been used to investigate disseminated neoplasia in molluscs (Elston et al. 1990, Moore et al. 1991, Reno et al. 1994. In the present study, a new method of cell lysis based on a hyposmotic shock has been developed and a combined comparison of the flow cytometry method, histology and chromosome analysis is reporte...

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“…Ainda em 1938, foi feita a primeira demonstração de que alguns produtos químicos poderiam ter ação estrogênica quando administrados em animais (7). Este assunto foi retomado mais recentemente devido ao surgimento de hipóteses que levantam a possibilidade de a exposição a agentes químicos ambientais, com ação estrogênica, poder estar relacionada ao aumento da incidência de câncer de mama (8,9), queda da quantidade de esperma, diminuição da fertilidade (10,11) defeitos congênitos secundários à exposição fetal (12) e outras alterações (13).…”

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Xenoestrogênios: o exemplo do bisfenol-A

Goloubkova

Spritzer

2000

Arq Bras Endocrinol Metab

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RESUMOEvidências acumuladas indicam que a saúde dos seres humanos, animais e espécies selvagens pode sofrer conseqüências adversas da exposição a produtos químicos presentes no meio ambiente e que interagem com o sistema endócrino, tais como bifenilas policloradas, dioxinas, estrogênios de ocorrência natural e sintéticos. Por outro lado, permanecem incertezas científicas com respeito aos dados relatados e, também, quanto à hipótese de haver níveis suficientemente elevados de exposição a estes agentes, a ponto de exercer efeito estrogênico generalizado sobre a população. Este trabalho revisa os principais tópi-cos relacionados a um dos xenoestrogênios que vem sendo mais recentemente estudado: o Bisfenol A (BFA), um monômero de plástico policarbonato, com pouca homologia estrutural com o estradiol (E 2 ) mas semelhante ao dietilestilbestrol (DES), hexestriol e componente bisfenóli-co do tamoxifeno. O presente trabalho comenta e analisa criticamente os efeitos do BFA sobre o trato reprodutivo e função lactotrófica em animais de experimentação, à luz das informações disponíveis e experiên-cia do grupo nesta área, e recomenda algumas necessidades de pesquisa. ABSTRACTSome evidences indicate that humans and domestic and wildlife species might suffer adverse consequences from exposure to environmental chemicals that interact with the endocrine system, including polychlorinated biphenyls, dioxins, synthetic and naturally occurring plant estrogens. However, considerable scientific uncertainty remains regarding the causes of these reported effects and whether sufficiently high levels of endocrine-disrupting chemicals exist in the ambient environment to exert adverse effects on the general population. This review summarizes the principal issues related to bisphenol A, an environmental endocrine disrupting chemical with estrogen activity. Bisphenol A is a monomer of plastics and has little structural homology with estradiol, sharing similarity with synthetic estrogens such as diethylstilbestrol and with the bisphenolic component of tamoxifen. In the light of available information and our laboratory experience in this field of research, this work comments and critically analyses the effects of BPA on the reproductive tract and lactotroph function in several rat strains, and also offers some recommendations for additional research.

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Is exposure to environmental or industrial endocrine disrupting estrogen-like chemicals able to cause genomic instability

Cited by 49 publications

References 56 publications

Infectious Disease and Contaminants in Urban Wildlife: Unseen and Often Overlooked Threats

Infectious Disease and Contaminants in Urban Wildlife: Unseen and Often Overlooked Threats

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis

Xenoestrogênios: o exemplo do bisfenol-A

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