Is gonadotropin stimulation bad for oocytes?

Lindenberg, Svend; Almind, Gitte Juul; Lindenberg, F

doi:10.1097/gco.0000000000000460

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…Controlled ovarian stimulation (COS) using exogenous gonadotropins (Gns) is a conventional step of the in vitro fertilization (IVF) treatment. With regard for this technique, oocyte retrieval is performed with an aim to harvesting a large number of oocytes to achieve a successful pregnancy (i.e., the more oocytes obtained per cycle, the more embryos can be selected for transfer) [1]. In recent years, the concept of acquiring as many oocytes as possible has been shifted to place emphasis on obtaining a cohort of high-quality embryos [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles

Yan

Song

et al. 2019

Reprod Biol Endocrinol

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Background Superovulation treatment had some adverse effects on maturity and development of oocytes. Can superovulation dose of gonadotropins (Gns) affect the transcriptome of granulosa cells and follicular fluid (FF) hormone levels? Methods One leading pre-ovulatory follicle per subject was used from three natural-cycle and four Gn-stimulated patients. Granulosa cells and FF samples were collected from the same leading follicle of each patient. RNA was extracted from granulosa cells and subjected to deep sequencing and analysis. Follicle-stimulating hormone (FSH), estradiol (E2), androstenedione (AND), testosterone (T), luteinizing hormone (LH), and progesterone (P4) levels in FF were measured by immunoassays. Student’s t test was used for statistical analysis. Results A total of 715 genes were up-regulated, and 287 genes were down-regulated, in the Gn-stimulated group relative to the control group. Gene Ontology analysis revealed that the down-regulated genes were enriched in cell cycle and meiosis pathways, primarily those associated with follicle or oocyte maturation and quality. On the other hand, the up-regulated genes were enriched in functions related to immunity and cytokine–cytokine receptor interactions. Compared to the follicles of natural cycle, the E2 and LH concentrations were significantly reduced ( P < 0.001), the P4 concentration was significantly increased ( P = 0.003), and the concentrations of FSH, T and AND had no difference in the follicles of Gn-stimulated cycle. Conclusions Cell cycle– and meiosis-associated genes were down-regulated by Gns stimulation, whereas immune- and cytokine-associated genes were up-regulated. Hormone levels were also affected by Gns stimulation. Compared with natural-cycle follicles,putative markers associated with oocyte quality and follicle maturation were significantly different from those in Gn-stimulated follicles. Hormone levels in follicles were compatible with the steroidogenic patterns of granulosa cell, which reflects the follicle maturation and oocyte quality. Electronic supplementary material The online version of this article (10.1186/s12958-019-0489-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles

Yan

Song

et al. 2019

Reprod Biol Endocrinol

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“…Assisted reproductive technology (ART) is widely used to conquer human infertility in the clinic. However, one major challenge of ART is how to retrieve a sufficient number of high‐quality oocytes to enhance the pregnancy outcome (Lindenberg, Almind, & Lindenberg, ). Ovarian stimulation or superovulation through the administration of exogenous hormones can promote follicular growth and oocyte maturation, thereby generating more oocytes (Fowler & Edwards, ).…”

Section: Introductionmentioning

confidence: 99%

Melatonin alleviates the deterioration of oocytes from mice subjected to repeated superovulation

Xiao

Nie

Wang

et al. 2019

Journal Cellular Physiology

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Induction of repeated superovulation with exogenous hormones is widely used in assisted reproductive technology (ART). Though it is generally safe, emerging evidence has indicated that repeated superovulation may compromise oocyte quality. However, few studies have explored how to ameliorate such impairment. Because melatonin has beneficial influences on oocytes in various detrimental environments, we aimed to explore whether melatonin could protect mouse oocytes after repeated superovulation. We found that repeated superovulation markedly reduced meiotic maturation and disrupted spindle organization and chromosome alignment. Furthermore, we observed reduced mitochondrial content and enhanced early apoptosis in oocytes from mice subjected to repeated superovulation. In addition, 5‐methylcytosine (5mc) fluorescence intensity was lower in oocytes from experimental mice than in those from control mice, indicating that repeated superovulation disrupts genomic DNA methylation, and elevations in reactive oxygen species levels indicated that repeated superovulation also induces oxidative stress. Conversely, melatonin administration improved oocyte maturation and attenuated the observed defects. Interestingly, supplementation with melatonin during in vitro maturation had the same protective effects on oocytes as in vivo melatonin administration. In summary, our results show that melatonin can improve oocyte quality after repeated superovulation and thus provide a potential strategy to improve ART efficiency.

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The effect of male factors on embryo morphokinetics: a retrospective analysis of 2726 blastocysts

Pellegrini,

Gatti,

Navarro

et al. 2024

J Assist Reprod Genet

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Is gonadotropin stimulation bad for oocytes?

Abstract: In conclusion, each fertility clinic setup has its own benefits and gonadotropin hyperstimulation in IVF has to be related to this and the specific patient demographic in the clinic; however, epigenetics and time to pregnancy are still issues open to debate.

Cited by 6 publications

References 17 publications

Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles

Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles

Melatonin alleviates the deterioration of oocytes from mice subjected to repeated superovulation

The effect of male factors on embryo morphokinetics: a retrospective analysis of 2726 blastocysts

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