The article presents the results of laboratory tests on the influence of the choice of the vehicle suspension position and the method of mounting child seats on the vibration comfort of children transported in them. Two child seats were used in the work. The B seat was attached to the vehicle with the ISOfix system, while the A seat was attached in the classic way (with seat belts). During the tests, the values of vertical vibrations were recorded on the seats of child seats, the rear seat of the vehicle and on the basis of ISOfix. The analyzed systems, depending on the method of mounting a child seat, may be characterized by two different vibration transmission chains. They depend on the method of fixing the child seat (the classic way of fixing the seat and the ISOFIX system). The article presents the results of empirical tests carried out at the EUSAMA SA.640 stand, which in these tests acted as a vibration generator with a frequency of 0 to 25 Hz. The analysis of the obtained results confirmed the observations published in previous articles about the negative impact of the use of the ISOfix base on the vibrational comfort of children.