Is the beneficial effect of urine extracts on mann-williamson ulcers due to the gastric secretory depressant in urine?

Sandweiss, David J.; Friedman, Michaël

doi:10.1007/bf02997292

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…Urogastrone was discovered after the observation that the extracts of urine from pregnant women had beneficial effect on healing of chronic ulcers in Mann-Williamson dogs. 3 Studies on animals showed that EGF, like urogastrone, displays many kinds of biological effects including stimulation of proliferation and differentiation of epithelial and non-epithelial tissues"4, stimulation of DNA synthesis4 through increasing ornithine decarboxylase activity,' protection of the gastroduodenal mucosa against various irritants, enhance-present in human saliva, urine and milk" and to be localised to the submandibular glands, Brunner's glands in the duodenum and the Paneth cells of the small intestine. '``Studies on the distribution of EGF in human gastrointestinal tract have, however, been limited mainly to immunocytochemistry and little information is available regarding the distribution and the release of EGF under various physiological conditions.…”

mentioning

confidence: 99%

Distribution and release of epidermal growth factor in man.

Konturek

Bielański

Konturek

et al. 1989

Gut

150

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SUMMARY Epidermal growth factor (EGF) is localised in man to salivary and Brunner's glands. It is present in large concentrations in saliva and duodenal contents but the mechanisms of its release have been little studied. This study carried out on four groups of healthy subjects was designed to determine the distribution and the release of immunoreactive EGF (IR-EGF) in salivary, gastric, duodenal, and pancreatic secretions. Under basal conditions, the concentrations of IR-EGF in salivary, gastric, duodenal and pancreatic secretions were; 2.7 (0.4), 0.42 (0.12), 21 (5) and 8.5 (1.2) ng/ml, respectively. Chewing of Parafilm* significantly increased salivary but not gastric or duodenal EGF output while atropinisation led to the reduction in basal salivary and duodenal EGF output without affecting the increment in EGF release induced by chewing. Cigarette smoking caused a marked reduction in basal salivary and duodenal EGF output. Infusion of pentagastrin increased salivary and duodenal EGF output and this was blocked by the addition of somatostatin. Injection of secretin lead to an increase in pancreatic output of EGF. We conclude that in man the major sources of EGF are salivary glands, duodenum, and pancreas and that the release of EGF remains under neurohormonal control.Epidermal growth factor (EGF) originally isolated by Cohen' from the mouse submaxillary glands is a 53 amino acid peptide structurally resembling urogastrone, another peptide isolated from urine.' Urogastrone was discovered after the observation that the extracts of urine from pregnant women had beneficial effect on healing of chronic ulcers in MannWilliamson dogs.3Studies on animals showed that EGF, like urogastrone, displays many kinds of biological effects including stimulation of proliferation and differentiation of epithelial and non-epithelial tissues"4, stimulation of DNA synthesis4 through increasing ornithine decarboxylase activity,' protection of the gastroduodenal mucosa against various irritants, enhancement of healing of chronic gastroduodenal ulceration`and inhibition of gastric acid secretion." Epidermal growth factor has been reported to be Address for correspondenIcice: Prof D)r S J Konturek. Institute of lPhysiology.31-531 Kr,tkow ul. Grzegorzecka 16. Poilnd.

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mentioning

confidence: 99%

Distribution and release of epidermal growth factor in man.

Konturek

Bielański

Konturek

et al. 1989

Gut

150

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“…From the start of investigations of a gastric inhibitory principle derived from urine some workers have considered urogastrone to be an antiulcer factor (Sandweiss, Saltzstein & Farbmann, 1939) but others have considered it to be an antisecretory factor (Gray, Wieczorowski & Ivy, 1939;Necheles, Hanke & Fantl, 1939). Sandweiss & Friedman (1942) concluded that the two activities were separate and named the antiulcer factor anthelone and the antisecretory factor urogastrone, but Lugaro, Lupi & Corbellini (1965) who studied the chemical and biological properties of a commercial preparation called Urogastrone considered that the two activities were probably due to the same substance. Even the definition of an antisecretory factor is uncertain, since some workers have assessed it by measuring inhibition of spontaneous secretion (e.g.…”

Section: Discussionmentioning

confidence: 99%

Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

Lawrence

Schild

Smith

1971

British J Pharmacology

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Summary1. Urogastrone, an acid gastric secretion inhibitor extracted from human urine, was assayed in the anaesthetized rat by circulating through its stomach a succinic-propionic buffer and recording electrometrically the secretion of acid in response to carbachol, and inhibition of carbachol secretion by urogastrone. 2. Quantitative assay procedures are described requiring the administration of only one or two doses of urogastrone to each rat.3. An extraction procedure for urogastrone is described based on the use of zeocarb, a cationic polystyrene resin.4. The most purified samples contained 40-80 times the amount/milligram which was just detectable by the assay method.

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“…As mentioned previously, the beneficial action of urogastrone on the healing of chronic peptic ulcerations has been described long ago (18), and it was clearly dissociated from the gastric antisecretory action of this substance. More recently, it has been confirmed that oral administration of synthetic human EGF does enhance the healing of chronic duodenal ulcerations induced by cysteamine (48).…”

Section: Egf and Ulcer Healingmentioning

confidence: 94%

“…Urogastrone was discovered after the observation that the extracts of urine from pregnant women had a beneficial effect on the healing of chronic ulcers in Mann-Williamson dogs (18). Since urogastrone given parenterally caused a potent inhibition of gastric acid secretion, the question remained whether the observed ulcer healing could be attributed to its antisecretory effect or to other action.…”

Section: Egf In Mucosal Protection and Integritymentioning

confidence: 98%