Is the biofilm formation and slime producing ability of coagulase-negative staphylococci associated with the persistence and severity of intramammary infection?

Simojoki, Heli; Hyvönen, Paula; Ferrer, C. Plumed; Taponen, Suvi; Pyörälä, Satu

doi:10.1016/j.vetmic.2012.02.031

Cited by 73 publications

(88 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ability to form biofilms was found to vary among coagulase--negative Staphylococcus species; S. xylosus and S. haemolyticus were strong biofilm formers, while S. simulans and S. epidermidis were weak biofilm formers. Simojoki et al (2012) found far fewer coagulase-negative staphylococci (31% of all CNS) forming a biofilm in the mammary gland of cows. The percentage of slime-producing CNS isolates was also small in this study: 14% produced slime on Congo Red Agar (CRA) with glucose, and 8.9% on CRA with lactose.…”

Section: Discussionmentioning

confidence: 94%

Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

Bochniarz¹,

Wawron²,

Szczubiał³

2014

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of the study was to evaluate the slime-producing ability of coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows. The study was carried out on 100 isolates of CNS obtained from milk of 86 cows from farms located in the Lublin region (Poland). Slime-producing ability was observed in over half of coagulase-negative staphylococci (54.0% of isolated CNS), including 19 isolates of methicillin-resistant staphylococci (95.5% of all MRCNS). Of 22 isolates of CNS responsible for the clinical form of mastitis, 20 isolates (90.9%) produced slime: S. xylosus (7 isolates), S. haemolyticus (6 isolates), S. chromogenes (4 isolates), and S. sciuri (3 isolates), including 9 isolates of MRCNS (45.0%). The remaining 34 isolates of CNS (43.6%) with the ability to produce this exopolysaccharide were isolated from the milk of cows with subclinical form of mastitis: S. xylosus (12 isolates), S. sciuri (9 isolates), S. chromogenes (6 isolates), S. haemolyticus (3 isolates), S. warneri (3 isolates) and S. saprophyticus (1 isolate), including 10 isolates of MRCNS (12.8%).

show abstract

Section: Discussionmentioning

confidence: 94%

Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

Bochniarz¹,

Wawron²,

Szczubiał³

2014

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Segundo estes autores, os achados expressaram a habilidade do micro-organismo para sobreviver aos mecanismos de defesa do hospedeiro, mesmo após o tratamento da mastite na secagem da ovelha. Em bovinos, a formação de biofilmes é uma possível causa pela qual estafilococos causadores de mastite evitam a resposta imune do animal e causam infecção intramamária persistente (20) , permanecendo na glândula mamária.…”

Section: Zafalon Lf Et Alunclassified

“…Esses resultados são interessantes, já que a produção de biofilmes por espécies coagulase-negativas foi classificada como rara, porém, segundo Simojoki et al (20), é mais comum em S. epidermidis que em outras espécies. Demais micro-organismos dessa espécie que não pertenciam a um mesmo grupo clonal sugerem uma distribuição ampla no rebanho em diferentes fontes de contaminação ou infecção.…”

Section: Zafalon Lf Et Alunclassified

Persistência De Staphylococcus Coagulase-Negativos Em Glândulas Mamárias De Ovelhas Com Mastite Subclínica Após O Tratamento Antimicrobiano À Secagem

et al. 2017

View full text Add to dashboard Cite

ResumoStaphylococcus coagulase-negativos (SCN) são os principais micro-organismos responsáveis pela mastite ovina e o tratamento ao final da lactação pode ser usado como método de controle contra a doença. Porém, o longo período seco apresentado pelas ovelhas em alguns sistemas de criação pode prejudicar os efeitos positivos do tratamento antimicrobiano. Os objetivos deste trabalho foram identificar as principais espécies de SCN na etiologia da mastite ovina antes e após o tratamento ao final da lactação das ovelhas, bem como investigar a persistência das espécies mais prevalentes na glândula mamária na lactação seguinte. Sessenta ovelhas foram divididas em dois grupos experimentais, um deles formado por animais sem tratamento antimicrobiano, enquanto o outro era composto por ovelhas cujas metades mamárias foram tratadas com cloxacilina-benzatina por via intramamária. As amostras de leite foram obtidas antes da secagem e aos 15 e 30 dias após o parto da lactação seguinte. As espécies prevalentes foram S. warneri, S. simulans e S. epidermidis. Clones das três espécies de maior ocorrência foram identificados antes e depois do tratamento, ou seja, mesmo com o extenso período seco entre as duas lactações consecutivas, os micro-organismos ainda foram identificados no interior da glândula mamária. Palavra-chave: bacteriologia; glândula mamária; leite ovino; métodos de controle da mastite. AbstractCoagulase-negative Staphylococci (CNS) are the main microorganisms responsible for mastitis in sheep and the treatment in the end of lactation can be used as a control method against disease. However, the long dry period presented by ewes in some breeding systems may impair the positive effects of antimicrobial treatment. The aims of this study were to identify the main CNS species in the etiology of ovine mastitis before and after treatment at dry-off of the ewes, as well as investigate the persistence of the most prevalent species in the mammary gland at the next lactation. Sixty sheep

show abstract

“…The mastitis treatment is based primarily on intramammary administration of antimicrobial agents to the animal, which may also be delivered by the parenteral route depending on the severity of the case. However, studies have shown that in many cases the success of the therapy is frustrated, being unable to eliminate the agent from the mammary gland, and allowing the development of persistent and recurrent cases of the disease (MELCHIOR et al, 2007;SIMOJOKI et al, 2012). One hypothesis to explain this fact is the ability of some microorganisms to form biofi lms on the surface of mammary gland tissue, thus presenting resistance to several antimicrobials (MELCHIOR et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland

et al. 2014

View full text Add to dashboard Cite

show abstract

Is the biofilm formation and slime producing ability of coagulase-negative staphylococci associated with the persistence and severity of intramammary infection?

Cited by 73 publications

References 32 publications

Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

Persistência De Staphylococcus Coagulase-Negativos Em Glândulas Mamárias De Ovelhas Com Mastite Subclínica Após O Tratamento Antimicrobiano À Secagem

Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland

Contact Info

Product

Resources

About