Is the pre-antral ovarian follicle the ‘holy grail’for female fertility preservation?

Bus, A.; Langbeen, A.; Martin, Bronwen; Leroy, Jo; Bols, P.E.J.

doi:10.1016/j.anireprosci.2019.05.017

Cited by 15 publications

(7 citation statements)

References 126 publications

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“…Then, the ovaries, fallopian tubes and uterus of the mouse were found through routine surgery after anesthesia. An incision was made in the fallopian tube, and embryos (9)(10)(11)(12)(13)(14)(15)(16) were injected into the fallopian tube through a microtubule at a time. After the wound was sutured, the mice were transferred to a suitable environment for culture until the mouse pups were born.…”

Section: Ivf Embryo Development In Vitro and In Vivo Transfermentioning

confidence: 99%

“…Among them, embryo cryopreservation requires women to have a male partner or use donated sperms, which may involve religious, moral, ethical and other issues 11 . Oocyte cryopreservation is mainly used by women who have reached adolescence and can withstand multiple rounds of ovarian stimulation 12 . For prepubescent girls and women in urgent need of cancer treatment, cryopreservation of ovarian tissue containing healthy follicles is the only option 13 .…”

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confidence: 99%

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Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

et al. 2022

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Preantral follicles are often used as models for cryopreservation and in vitro culture due to their easy availability. As a promising approach for mammalian fertility preservation, vitrification of preantral follicles requires high concentrations of highly toxic penetrating cryoprotective agents (up to 6 M). Here, we accomplish low-concentration-penetrating cryoprotective agent (1.5 M) vitrification of mouse preantral follicles encapsulated in hydrogel by nanowarming. We find that compared with conventional water bath warming, the viability of preantral follicles is increased by 33%. Moreover, the cavity formation rate of preantral follicles after in vitro culture is comparable to the control group without vitrification. Furthermore, the percentage of MII oocytes developed from the vitrified follicles, and the birth rate of offspring following in vitro fertilization and embryo transfer are also similar to the control group. Our results provide a step towards nontoxic vitrification by utilizing the synergistic cryoprotection effect of microencapsulation and nanowarming.

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Section: Ivf Embryo Development In Vitro and In Vivo Transfermentioning

confidence: 99%

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confidence: 99%

Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

et al. 2022

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“…Freezing of oocytes is more complicated due to limited availability and the large cell size compared with spermatozoa. Nevertheless, refinement in techniques and devices nowadays allows preservation of both sperm and oocytes; however, freezing ovarian follicles is still a challenge (13). The increasing clinical interest in cryopreservation of oocyte has led to studies of the biophysical parameters of mammalian oocyte membrane permeability to water and cryoprotectant agents (21,59,60,69).…”

Section: Cryopreservation Of Gametes and Embryosmentioning

confidence: 99%

Biomechanics of Early Life in the Female Reproductive Tract

2020

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“…Premature subfertility, by itself, has a negative effect on the quality of life in these patients, and hence, fertility preservation plays a key role in this regard. There are quite a number of ways patented to preserve fertility in cancer patients like oocyte, embryo and ovarian tissue cryopreservation [ 2 ]. However, ovarian tissue cryopreservation, by itself, may result in its own restrictions while being applied for critical cancer conditions due to its contamination with malignant cells, which potentiates recurrence of cancer in the patient following ovarian tissue transplantation.…”

Section: Introductionmentioning

confidence: 99%

Successful 3D culture and transplantation of mouse isolated preantral follicles in hydrogel of bioengineered Wharton’s jelly

Zand,

Rajablou,

Siadat

et al. 2023

PLoS ONE

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Main objective Due to Human Wharton’s Jelly (HWJ) could be applied in tissue engineering as a bio scaffold, the present study was conducted to investigate the effects of HWJ hydrogel on in vitro culture and auto-transplantation of mouse ovarian follicles. Materials and methods HWJ was isolated from umbilical cord and decellularized with SDS/Tris/EDTA. DNA, Collagen and Glycosaminoglycans (GAGs) were measured. Decellularized Wharton’s Jelly (DWJ) was dissolved to make Wharton’s Jelly Hydrogel (WJH), and composited with Alginate (ALG) (1.5%) in equal ratio (WJH+ALG). Then, mouse preantral follicles were isolated and encapsulated in 10μL droplets of WJH and randomly considered for both 14 days culture and auto-transplantation. Results Collagen, GAGs and DNA evaluations showed majority of WJ cells have been removed and MTT approved no toxicity. Degradation rate and rheological analysis represented optimal hydrogel compatibility. The data from in vitro culture revealed significant antral formation in WJH+ALG (P≤0.05). In transplantation, follicles failed to survive in ALG; however, survived in WJH+ALG to antral stage (P<0.05). VEGF and CD34 had greater expression in WJH+ALG than ALG (P< 0.05). Conclusion Wharton’s jelly hydrogel and Alginate compound is interesting composite for successful development of mouse preantral follicles in both 3D in vitro culture and transplantation.

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Is the pre-antral ovarian follicle the ‘holy grail’for female fertility preservation?

Cited by 15 publications

References 126 publications

Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Biomechanics of Early Life in the Female Reproductive Tract

Successful 3D culture and transplantation of mouse isolated preantral follicles in hydrogel of bioengineered Wharton’s jelly

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