Is there an optimal method to detach Candida albicans biofilm from dental materials?

Abstract

Introduction. Candida albicans can produce a complex, dynamic and resistant biofilm on the surface of dental materials, especially denture base acrylic resins and temporary soft liners. This biofilm is the main aetiological factor for denture stomatitis, an oral inflammatory condition characterized by chronic and diffuse erythema and oedema of the denture bearing mucosa. Gap Statement. There is no consensus in the literature regarding the best method to detach biofilms from dental materials. In order to assess the antifungal efficacy of new materials and treatments, the biofilm needs to be properly detached and quantified. Aim. This study compared different methods of detaching C. albicans biofilm from denture base acrylic resin (Vipi Cril) and temporary soft liner (Softone) specimens. Methodology. Specimens of each material were immersed in an inoculum of C. albicans SC5314 and remained for 90 min in orbital agitation at 75 r.p.m. and 37 °C. After the removal of non-adherent cells, the specimens were immersed in RPMI-1640 medium for 48 h. Biofilm formation was evaluated with confocal laser scanning microscopy (n=5). Then, other specimens (n=7) were fabricated, contaminated and immersed in 3 ml of sterile phosphate-buffered saline (PBS) and vortexed or sonicated for 1, 2, 5, or 10 min to detach the biofilm. The quantification of detached biofilm was performed by colony-forming unit (c.f.u.) ml−1 count. Results were submitted to one-way analysis of variance (ANOVA)/Tukey HSD test (α=0.05). Results. A mature and viable biofilm was observed on the surfaces of both materials. For both materials, there was no significant difference (P>0.05) among detachment methods. Conclusion. Any of the tested methods could be used to detach C. albicans biofilm from hard and soft acrylic materials.

A Temporary Acrylic Soft Denture Lining Material Enriched with Silver-Releasing Filler-Cytotoxicity, Mechanical and Antifungal Properties

“…Future experiments with biofilm formation should give a better reference of results to real conditions and look particularly promising if we consider that experimental soft linings with antimicrobial fillers have not been investigated in that context until now. In addition, the available results refer to a biofilm based on C. albicans as the most important pathogenic microorganism [50], when synergistic interactions between different microorganisms can also have an influence on the condition of the polymicrobial biofilm [51,52].…”

The Effect of Exposure to Candida Albicans Suspension on the Properties of Silicone Dental Soft Lining Material

“…Due to the conditions under dentures, we need further experiments taking into account environmental conditions, such as different temperature values and pH affecting the germ-tube formation process. Another limitation was that a basic suspension of C. albicans was used, so future experiments after biofilm formation, including the possibility of synergistic interactions between various microorganisms [16,85,86], will pose a serious challenge. Attention should be paid to the lack of prior research studies on the topic, and objective methodological difficulties that could potentially influence the results of microscopic studies.…”