IS1 is involved in deletion formation in the gal region of E. coli K12

Reif, H. J.; Saedler, Heinz

doi:10.1007/bf00332538

Cited by 229 publications

(62 citation statements)

References 27 publications

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“…Indeed, a deletion of some gvpA genes is found next to the insertion of this IS in the mutant M1 [1]. Such coupled insertion/deletion events have already been described for IS1 elements in the gal region [20] and in the lactose transposon [21] of E. coli. Illegitimate recombination driven by IS1 has been suggested as a mechanism to explain this phenomenon.…”

Section: Indeed Several Examples Of Translational Slippage In Asupporting

confidence: 53%

Mobile DNA elements in the gas vesicle gene cluster of the planktonic cyanobacteria Microcystis aeruginosa

Mlouka

Comte

Marsac

2004

FEMS Microbiology Letters

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Insertion sequences (IS) have been characterized in Microcystis aeruginosa gas vesicle-deficient mutants. ISMae4, a homolog of the cyanobacterial IS702, belongs to the IS5 family, subgroup ISL2. ISMae2 and ISMae3 display typical IS features and express a transposase of the IS4 and IS1 family, respectively. ISMae1 exhibits a more complex genetic structure and harbours a degenerated transposase gene distantly related to IS1 elements. Hybridizations with IS-specific DNA probes suggest that transposition of ISMae2 and ISMae3 occurred by a replicative-type mechanism. To our knowledge this is the first report showing that IS1 elements can be mobile in cyanobacteria.

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Section: Indeed Several Examples Of Translational Slippage In Asupporting

confidence: 53%

Mobile DNA elements in the gas vesicle gene cluster of the planktonic cyanobacteria Microcystis aeruginosa

Mlouka

Comte

Marsac

2004

FEMS Microbiology Letters

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“…Several early studies addressed the effect of another environmental factor, temperature. In particular, it was demonstrated that a copy of IS1 inserted in the gal operon of Escherichia coli generated adjacent deletions whose appearance was sensitive to an increase in temperature (Reif and Saedler, 1975). A similar temperature sensitivity was observed in the case of intermolecular transposition of the transposon Tn3 in E. coli (Kretschmer and Cohen, 1977) and of the Tn3-related lac transposon Tn951 in both E. coli and Y. enterocolitica (Cornelis, 1980).…”

Section: Introductionmentioning

confidence: 65%

“…Several early studies on other transposable elements concluded that transposition was temperature sensitive. In one study, the insertion sequence, IS1, exhibited a pronounced temperature sensitivity in generating adjacent deletions (Reif and Saedler, 1975). In this case, IS1 was inserted into the gal operon.…”

Section: Discussionmentioning

confidence: 99%

IS911‐mediated intramolecular transposition is naturally temperature sensitive

Haren

Bétermier

Polard

et al. 1997

Molecular Microbiology

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SummaryIt is shown here that the bacterial insertion sequence IS911 exhibits a temperature-sensitive transposition phenotype. Previous results have demonstrated that elevated levels of the IS911 transposase OrfAB generate significant quantities of a figure-eight form, created by cleavage and circularization of one of the transposon strands, and of an excised circular form, in which both transposon strands have been circularized. We show here that the level of both types of molecule observed in vivo was greatly reduced at 42ЊC compared with 37ЊC. On the other hand, reducing the temperature to 30ЊC resulted in a significant increase in production. Transposition activity at this temperature was sufficiently high to permit detection in vivo of an excised circular form of a defective single IS911 chromosomal copy when OrfAB is supplied in trans. A similar temperature-activity profile is observed for a cell-free reaction that uses partially purified OrfAB and generates the figure-eight form uniquely. Moreover, two point mutants of OrfAB were obtained, which render the reactions partially temperature resistant both in vivo and in vitro. These results suggest that some property of transposase itself is sensitive to elevated temperatures.

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“…ally, point mutations or small DNA rearrangements may restore the function of the mutated gene converting the cryptic transposon to a normal one. Alternatively, a gene within an IS flanked transposon may be mutated due to the activities of its IS elements, particularly IS-mediated deletion (Reif and Saedler, 1975;lida and , converting an active transposon into one that is phenotypically silent. Again, further spontaneous mutation may restore the phenotype.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Lida¹,

Marcoli²,

Bickle³

1982

The EMBO Journal

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We have physically characterised a deletion mutant of the R plasmid R100 which has lost all of the antibiotic resistances, including chloramphenicol resistance (Cmr), coded by its IS1‐flanked r‐determinant. The deletion was mediated by one of the flanking IS1 elements and terminates within the carboxyl terminus of the Cmr gene. DNA sequence analysis showed that the mutated gene would produce a protein 20 amino acids longer than the wild‐type due to fusion with an open reading frame in the IS element. Surprisingly for a deletion mutation, rare, spontaneous Cmr revertants could be recovered. Two of the four revertants studied had frame shifts due to the insertion of a single AT base pair at the same position; the revertants would code for a protein five amino acids shorter than the wild‐type. The other two revertants had acquired duplications of the 34‐bp inverted terminal repeat sequences of the IS1 element and would direct the synthesis of a protein six amino acids longer than the wild‐type. The reverted Cmr markers were still capable of transposition. These observations suggest a role for point mutations and small DNA rearrangements in the formation of new gene organisations produced by mobile genetic elements.

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IS1 is involved in deletion formation in the gal region of E. coli K12

Cited by 229 publications

References 27 publications

Mobile DNA elements in the gas vesicle gene cluster of the planktonic cyanobacteria Microcystis aeruginosa

Mobile DNA elements in the gas vesicle gene cluster of the planktonic cyanobacteria Microcystis aeruginosa

IS911‐mediated intramolecular transposition is naturally temperature sensitive

Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

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