Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Lida, Shojaei Kavan; Marcoli, Roberto; Bickle, Thomas A.

doi:10.1002/j.1460-2075.1982.tb01242.x

Cited by 10 publications

(2 citation statements)

References 51 publications

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“…The line KK/VR‐40b carries a Tip100 derivative, Tip100–1 , which contains an additional 48 bp terminal sequence as tandem repeats. A similar duplication of 34 bp terminal sequence in the prokaryotic transposable IS 1 element has been reported previously (Iida et al . 1982).…”

Section: Discussionsupporting

confidence: 83%

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

1998

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SummaryThe mutable flaked (or a f ) lines of the common morning glory bear white flowers with colored flakes and sectors. The a f allele shows incomplete dominance. Plants in the heterozygous state A/a f bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable a f allele is caused by insertion of a new transposable element, Tip100, into the CHS-D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100-1. The structure of Tip100-1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100. Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS-D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.

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Section: Discussionsupporting

confidence: 83%

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

1998

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“…A similar duplication of 34‐bp terminal sequence in the prokaryotic transposable IS1 element was reported. 43 Except for the subline carrying an additional secondary mutation in Tip100 to generate Tip 100 ‐1, all the mutable flaked lines we examined contain the identical CHS‐D structure with Tip 100 insertion.…”

Section: The Mutable Flaked Allelementioning

confidence: 99%

Floricultural Traits and Transposable Elements in the Japanese and Common Morning Gloriesa^a

Iida

Hoshino

Johzuka-Hisatomi

et al. 1999

Annals of the New York Academy of Sciences

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The Japanese morning glory has an extensive history of genetic studies and over 200 different spontaneous mutant lines have been described. Of these, we identified that two mutable alleles, flecked and speckled, for flower variegations are caused by integration of transposable elements, belonging to the En/Spm family, into the DFR-B and CHI genes for flower pigmentation, respectively. The mutable flaked allele of the common morning glory bearing variegated flowers is caused by insertion of a new transposable element, Tip100, into one of the CHS genes for pigmentation and that Tip100 belongs to the Ac/Ds family. These results are discussed with regard to spontaneous transposon mutagenesis and generation of floricultural traits of morning glories.

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The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

Suri¹,

Shepherd²,

Bickle³

1984

The EMBO Journal

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been isolated. It proves to be an unusual enzyme, clearly related functionally to the classical type I restriction enzymes. The basic enzyme is a two subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. The DNA recognition sequence of EcoA has an overall structure that is very similar to previously determined type I sequences. It is: 5'-GAGNNNNNNNGTCA-3' 3'-CTCNNNNNNNCAGT-5' where N can be any nucleotide. Modification methylates the adenosyl residue in the specific trinucleotide and the adenosyl residue in the lower strand of the specific tetranucleotide.

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Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Cited by 10 publications

References 51 publications

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

Floricultural Traits and Transposable Elements in the Japanese and Common Morning Gloriesa^a

The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

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Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Cited by 10 publications

References 51 publications

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

Molecular characterization of the mutableflakedallele for flower variegation in the common morning glory

Floricultural Traits and Transposable Elements in the Japanese and Common Morning Gloriesaa

The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

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Product

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Floricultural Traits and Transposable Elements in the Japanese and Common Morning Gloriesa^a