Isobolographic analysis of interaction between intrathecal morphine and clonidine in the formalin test in rats

Przesmycki, Krzysztof; Dzieciuch, Jacek A; Czuczwar, Stanisław J.; Kleinrok, Z

doi:10.1016/s0014-2999(97)01280-6

Cited by 32 publications

(11 citation statements)

References 31 publications

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“…Intrathecal morphine reduced the flinching response in both phases of the formalin test in the present study, corroborating with previous results (11-14). Therefore, opioid receptors are involved in the modulation of acute pain as well as the facilitated state.…”

Section: Discussionsupporting

confidence: 94%

“…Recently, it has been reported that intrathecal sildenafil produced an antinociception, which is mediated through the nitric oxide (NO)-cGMP pathway (9, 10). It is demonstrated that morphine reversed not only acute nociception but also tissue injury hyperalgesia through the action on spinal opioid receptor (11-14). Furthermore, several lines of evidence suggest that opioid-induced antinociception may be related to the activation of the NO-cGMP pathway (15-17).…”

Section: Introductionmentioning

confidence: 99%

“…The purpose of the present study was to evaluate the characteristics of pharmacological interaction between spinal sildenafil and morphine in the formalin test which shows tissue injury pain leading to the facilitated state as well as acute pain (11). We further clarified the possibility of contribution of spinal opioid receptor on the action of sildenafil.…”

Section: Introductionmentioning

confidence: 99%

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Additive Antinociception between Intrathecal Sildenafil and Morphine in the Rat Formalin Test

et al. 2008

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The possible characteristics of spinal interaction between sildenafil (phosphodiesterase 5 inhibitor) and morphine on formalin-induced nociception in rats was examined. Then the role of the opioid receptor in the effect of sildenafil was further investigated. Catheters were inserted into the intrathecal space of male Sprague-Dawley rats. For induction of pain, 50 µL of 5% formalin solution was applied to the hind-paw. Isobolographic analysis was used for the evaluation of drug interaction between sildenafil and morphine. Furthermore, naloxone was intrathecally given to verify the involvement of the opioid receptor in the antinociception of sildenafil. Both sildenafil and morphine produced an antinociceptive effect during phase 1 and phase 2 in the formalin test. The isobolographic analysis revealed an additive interaction after intrathecal delivery of the sildenafil-morphine mixture in both phases. Intrathecal naloxone reversed the antinociception of sildenafil in both phases. These results suggest that sildenafil, morphine, and the mixture of the two drugs are effective against acute pain and facilitated pain state at the spinal level. Thus, the spinal combination of sildenafil with morphine may be useful in the management of the same state. Furthermore, the opioid receptor is contributable to the antinocieptive mechanism of sildenafil at the spinal level.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Additive Antinociception between Intrathecal Sildenafil and Morphine in the Rat Formalin Test

et al. 2008

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“…Morphine has been shown to produce dose-dependent inhibition of both phases in the formalin test with ID 50 values of 0.66 and 4.1 nmol, respectively (Przesmycki et al, 1997).…”

Section: +mentioning

confidence: 99%

The antinociceptive and anti-inflammatory effects ofSalvia officinalisleaf aqueous and butanol extracts

Qnais

Abu-Dieyeh

Abdulla

et al. 2010

Pharmaceutical Biology

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“…Adrenergic and opioid agonists are antinociceptive when intrathecally injected either alone or in combination (Kuraishi et al, 1985;Przesmycki et al, 1997;Sherman et al, 1988). Likewise, chromaffin cells release catecholamines and opioid peptides and the antinociceptive effects are blocked with either phentolamine or naloxone (Czech and Sagen, 1995;Siegan and Sagen, 1997;Vaquero et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Spinal subarachnoid adrenal medullary transplants reduce hind paw swelling and peripheral nerve transport following formalin injection in rats

2008

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Previous studies have demonstrated that adrenal medullary chromaffin cells transplanted into the spinal subarachnoid space significantly reduced pain-related behavior following hind paw plantar formalin injection in rats. The data suggests a centrally mediated antinociceptive mechanism. The spinal transplants may have effects on sciatic nerve function as well. To address this, the current study examined the effects of spinal adrenal transplants on hind paw edema and the anterograde transport of substance P (SP) that occur following formalin injection. Robust formalin-evoked edema, as well as hind paw flinching, was observed in striated muscle control-transplanted rats, which were not observed in adrenal-transplanted rats. To visualize transport of SP, the sciatic nerve was ligated ipsilateral to formalin injection and the nerve was processed 48 h later for immunocytochemistry. A significant formalin-induced accumulation of SP immunoreactivity (IR) was observed proximal to the ligation in control-transplanted rats. In contrast, there was significantly less SP IR observed from nerve of adrenal-transplanted rats, suggesting a diminution of anterograde axoplasmic transport by adrenal transplants. The change in SP IR may have been due to an alteration of transport due to formalin injection, thus, transport was visualized by the accumulation of growth-associated protein 43 (GAP43) at the ligation site. Formalin injection did not significantly increase proximal accumulation of GAP43 IR, indicating that formalin does not increase anterograde transport. Surprisingly, however, adrenal transplants significantly diminished GAP43 IR accumulation compared to control-transplanted rats. These data demonstrate that spinal adrenal transplants can attenuate the formalin-evoked response by modulating primary afferent responses.

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Isobolographic analysis of interaction between intrathecal morphine and clonidine in the formalin test in rats

Cited by 32 publications

References 31 publications

Additive Antinociception between Intrathecal Sildenafil and Morphine in the Rat Formalin Test

Additive Antinociception between Intrathecal Sildenafil and Morphine in the Rat Formalin Test

The antinociceptive and anti-inflammatory effects ofSalvia officinalisleaf aqueous and butanol extracts

Spinal subarachnoid adrenal medullary transplants reduce hind paw swelling and peripheral nerve transport following formalin injection in rats

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