Isobutanol production by combined in vivo and in vitro metabolic engineering

Gupta, Mamta; Wong, Matthew S.; Jawed, Kamran; Gedeon, Kamil S.; Barrett, Hannah; Bassalo, Marcelo C.; Morrison, Clifford S.; Eqbal, Danish; Yazdani, Syed Shams; Gill, Ryan T.; Huang, Jiaqi; Douaisi, Marc; Dordick, Jonathan S.; Belfort, Georges; Koffas, Mattheos A. G.

doi:10.1016/j.mec.2022.e00210

Cited by 5 publications

(1 citation statement)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integration of the α-KIV pathway was performed using CRISPR-Cas9 technique, followed by the downregulation of the competitive valine biosynthetic pathway through CRISPR technology. This simultaneous dual genetic approach aided in the generation of 1.78 g/L of isobutanol with 93% yield and productivity of 0.07 g/L/h [26].…”

Section: Isobutanolmentioning

confidence: 99%

Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production

2023

View full text Add to dashboard Cite

Sustainable biofuel production is necessary to meet the daunting challenge of “fueling” growing economies with a significantly reduced carbon footprint. Although its higher oxygen content often hinders the direct conversion of lignocellulosic biomass (LCB) into energy-dense biofuels, microbial biofuel production from LCB still has potential. The production of primary alcohols by acetone–butanol–ethanol (ABE) fermentation has been practiced for more than a century to attain near-theoretical maximum. However, ABE produced conventionally by native microorganisms is not equivalent to fossil fuel-based aviation fuels in terms of energy density, volatility, and cost-efficiency. Various strategies have been adapted for the microbial synthesis of advanced fuels from renewable feedstock with the advancements in genetic engineering. Yet, the presence of inhibitors and the inefficiency of microbes to utilize or transport the sugar mixtures from LCB often impede titer and yield. However, ABE mixtures can act as platform chemicals to synthesize high-value biofuels by biocatalytic or chemo-catalytic applications. Chemical catalysts, in particular, are used to produce higher alcohols ranging from 3-carbon to 20-carbon fuels from the ABE fermentation mixture. This article reviews the recent trends in the production of higher biofuels from ABE mixtures using biological and chemical catalysts. Focus is placed on genomic and metabolic engineering strategies implemented to upgrade microbes for higher biofuel production via the fermentation of renewable feedstocks. This paper also summarizes the advancements in the chemical conversion route of an ABE fermentation mixture into higher biofuels. Finally, the review provides insights into future research toward commercializing renewable and sustainable higher biofuels and chemicals.

show abstract

Section: Isobutanolmentioning

confidence: 99%

Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production

2023

View full text Add to dashboard Cite

show abstract

Microbial host engineering for sustainable isobutanol production from renewable resources

Nawab,

Zhang,

Ullah

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Biotechnology and biofuels: paving the way towards a sustainable and equitable energy for the future

Nath

2024

Discov Energy

View full text Add to dashboard Cite

This comprehensive review aims to explore and elucidate the pivotal role of biotechnology in biofuel production, specifically focusing on its contribution to enhancing sustainability, efficiency, and productivity in the energy sector. By examining various biotechnological approaches like genetic engineering, metabolic engineering, and synthetic biology, it seeks to provide insights into effectively harnessing biofuel generation processes, including the integration of machine learning and life cycle assessment for microalgae cultivation and harvesting. Additionally, it sheds light on the multifaceted implications surrounding biofuel production and consumption, addressing technological, ethical, social, and economic considerations. Through critical analysis of the advantages and challenges associated with biotechnology-driven biofuel development, it offers a balanced perspective on the true potential of biofuels as a viable, sustainable, and equitable energy source for the future. This examination provides a holistic analysis of the symbiotic relationship between biotechnology and biofuels, highlighting how advancements in biotechnological techniques can pave the way for a more sustainable and resilient energy future. By addressing both the promises and limitations of biotechnology in this context, it aims to contribute to informed decision-making and policy formulation to drive the transition toward a cleaner and more equitable energy paradigm. Graphical Abstract

show abstract

Isobutanol production by combined in vivo and in vitro metabolic engineering

Cited by 5 publications

References 62 publications

Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production

Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production

Microbial host engineering for sustainable isobutanol production from renewable resources

Biotechnology and biofuels: paving the way towards a sustainable and equitable energy for the future

Contact Info

Product

Resources

About