Isocratic reporter-exclusion immunoassay using restricted-access adsorbents

Abstract

We introduce analyte-dependent exclusion of reporter reagents from restricted-access adsorbents as the basis of an isocratic reporter-exclusion immunoassay for viruses, proteins, and other analytes. CaptoTM Core 700 and related resins...

Smartphone-read phage lateral flow assay for point-of-care detection of infection

“…Uncoupled antibodies were removed by passing the solution through a Capto Core 700 resin (Cytiva) column (2 mL) pre-equilibrated with 1× phosphate-buffered saline solution (PBS). 34 Phage construct concentration was determined using UV-Vis absorbance (Thermo NanoDrop ND-1000 UV/Vis Spectrophotometer) and the formula: virions per mL = [( A 269nm − A 320nm ) × 6 × 10 16 ]/genome length 35 (7249 nt for M13mp18). Phage constructs were stored in 1× PBS at 4 °C until use.…”