Isoenzyme Pattern of Lactate Dehydrogenase Associated with Human Prostasomes

Olsson, Ingmar; Ronquist, Gunnar

doi:10.1159/000281735

Cited by 15 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prostasomes contain many small molecules and ions (Ca 2+ , Zn 2+ , GDP, ADP and ATP) and a number of enzymes among which phopholipase A, ATPase and peptidases [18][19][20][21][22][23], to list only few. Many prostasomal proteins have been recently identified through the analysis of proteome by Utleg et al [24].…”

Section: Prostasome Componentsmentioning

confidence: 99%

Role of human prostasomes in the activation of spermatozoa

Arienti¹,

Carlini²,

Saccardi

et al. 2004

J Cellular Molecular Medi

View full text Add to dashboard Cite

Prostasomes are small vesicles of prostatic origin contained in human semen. Their composition is peculiar under many aspects. Cholesterol is abundant and many proteins are endowed with enzymatic or other activities. The function of prostasomes has been amply debated and several hypotheses have been put forward. The liquefaction of semen, spermatozoa motility, antibacterial activity and immunological functions have been related to prostasomes. Under certain aspects, prostasomes resemble synaptosomes. The fusion of prostasomes to spermatozoa enriches spermatozoa with cholesterol and causes bursts of cytoplasmic sperm calcium. The interaction of spermatozoa and prostasomes should be limited to vagina since prostasomes are immobile and do not follow spermatozoa in the superior female genital tract. Calcium bursts would increase spermatozoa motility, where cholesterol would decapacitate spermatozoa, so preventing untimely activation. Since spermatozoa receive many different molecules from prostasomes, additional effects are also possible. Prostasomes makes spermatozoa more apt to be activated by progesterone in the proximity of the ovum. Therefore, the fusion between spermatozoa and prostasomes would influence spermatozoa behaviour under many aspects and might be relevant for fecundation. The richness of molecular species in prostasomes is amazing and these small vesicles are expected to lead to many more discoveries in the field of human reproduction.

show abstract

Section: Prostasome Componentsmentioning

confidence: 99%

Role of human prostasomes in the activation of spermatozoa

Arienti¹,

Carlini²,

Saccardi

et al. 2004

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…For example, epididymosomes (Wilson et al 1984) are derived from epididymal epithelial cells, and other vesicles are further provided by the vesicular glands and by the vasa deferentia (Lilja and Weiber 1983;Wilson et al 1984). However, while it would be certainly more appropriate to use the term prostasome for the vesicles produced by the prostatic epithelium only (Olsson and Ronquist 1990), this term is often applied to the whole variety of membrane vesicles isolated from seminal plasma (Stegmayr and Ronquist 1982). The lipid composition of these vesicles is peculiar; cholesterol is present in high amounts as is sphingomyelin, whereas phosphatidylcholine is less abundant (Arienti et al 1998;Arvidson et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Identification and Partial Characterization of Two Populations of Prostasomes by a Combination of Dynamic Light Scattering and Proteomic Analysis

Chiasserini

Mazzoni²,

Bordi

et al. 2015

J Membrane Biol

View full text Add to dashboard Cite

Prostasomes are vesicles secreted by prostate epithelial cells and are found in abundance in the semen. Here we characterized two different prostasome populations isolated from human seminal fluid. Prostasomes were isolated using differential centrifugation, while dynamic light scattering (DLS) was used to characterize their size and size distribution. Their protein content was analyzed using two-dimensional electrophoresis and mass spectrometry. DLS showed two distinct prostasome subpopulations in centrifuged seminal plasma, with an average hydrodynamic radius of 80 and 300 nm. The larger population was isolated after centrifugation at 20,000 × g (P20), while the smaller one was recovered at 100,000 × g (P100). The two fractions had a similar lipid composition, showing an elevated content of sphingomyelin and cholesterol. The P100 vesicles showed a significant over-expression of proteins involved in the endosomal sorting complexes required for transport (ESCRT) machinery such as Alix, TSG101, and syntenin-1. Some proteins possibly involved in prostate cancer were present only in one specific population (TMPRSS2 in P100 and VCP in P20). The different size and protein profile in the two subpopulations of prostasomes might support differential roles of the semen vesicles toward the target cells, and/or different secretion pathways from the organ of origin.

show abstract

“…An ATPase system may be responsible for the transport of Ca 2+ into prostasomes [8]. Furthermore, gamma-glutamyltransferase [9], lactate dehydrogenase [10], and angiotensin converting enzyme [11] are associated with the prostasome membrane. An enzyme that was found in an extremely high concentration on prostasomes was dipeptidyl peptidase IV (DPP IV) [12].…”

Section: Introductionmentioning

confidence: 99%