2003
DOI: 10.1152/ajpcell.00461.2002
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Isoenzyme-selective regulation of SERCA2 gene expression by protein kinase C in neonatal rat ventricular myocytes

Abstract: marel. Isoenzyme-selective regulation of SERCA2 gene expression by protein kinase C in neonatal rat ventricular myocytes. Am J Physiol Cell Physiol 285: C39-C47, 2003. First published February 26, 2003 10.1152/ajpcell.00461. 2002-Patients with cardiac hypertrophy and heart failure display abnormally slowed myocardial relaxation, which is associated with downregulation of sarco(endo)plasmic reticulum Ca 2ϩ -ATPase (SERCA2) gene expression. We previously showed that SERCA2 downregulation can be simulated in cu… Show more

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Cited by 37 publications
(39 citation statements)
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“…Nevertheless, overexpression of PKCδ did not appear to alter MARCKS phosphorylation. Levels of overexpressed PKCε, PKCα, and PKCδ are also shown, and are consistent with the levels of PKC overexpression obtained in previous studies from our laboratory (21,27). Data summarizing the results of 4-5 experiments following caPKCε expression in NRVM and ARVM are depicted in Fig 2D. …”
Section: Et Treatment Increases Marcks Phosphorylation In Nrvm and Arvmsupporting
confidence: 89%
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“…Nevertheless, overexpression of PKCδ did not appear to alter MARCKS phosphorylation. Levels of overexpressed PKCε, PKCα, and PKCδ are also shown, and are consistent with the levels of PKC overexpression obtained in previous studies from our laboratory (21,27). Data summarizing the results of 4-5 experiments following caPKCε expression in NRVM and ARVM are depicted in Fig 2D. …”
Section: Et Treatment Increases Marcks Phosphorylation In Nrvm and Arvmsupporting
confidence: 89%
“…We have also reported cross-talk between PKCα and PKCε. Overexpression of dnPKCε, even at moderate levels, reduced endogenous PKCα levels in NRVM (21). Conversely, overexpression of dnPKCα decreased endogenous PKCε levels.…”
Section: Discussionmentioning
confidence: 89%
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“…RNA was quantified by absorbance at 260 nm and its integrity was determined by examining the 28S and 18S rRNA bands in ethidium bromide-stained agarose gels. SERCA2, ANF, αMHC, and βMHC mRNAs were then analyzed by real-time RT-PCR, as previously described (5,28). The mixture consisted of 1µL of sample cDNA, 21µL DEPC water, 25µL Platinum Quantitative PCR SuperMix-UDG, and 3µL of a primer/dual-labeled probe combination specific for each gene of interest (Table 1).…”
Section: Mrna Analysismentioning
confidence: 99%
“…All samples were run in triplicate, and the results were averaged. Data were analyzed as previously described (28).…”
Section: Mrna Analysismentioning
confidence: 99%