Isoflavone and the heme oxygenase system in ischemic acute kidney injury in rats

Watanabe, Mirian; Neiva, Luciana Barros de Moura; Santos, Célio Xavier da Costa; Laurindo, Francisco Rafael Martins; Vattimo, Maria de Fátima Fernandes

doi:10.1016/j.fct.2007.06.013

Cited by 27 publications

(31 citation statements)

References 29 publications

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“…23,31,34,35 However, no study has investigated the effects of an immunomodulatory agent, such as infliximab, on I/R damage in rat kidneys. Infliximab, a humanized mouse monoclonal antibody to TNF-α, is a new immunomodulatory agent.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

et al. 2012

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Objective: To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney. Methods: Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R þ infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R þ infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections. Results: The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R þ infliximab (bi) and I/R þ infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R þ infliximab (bi) group and the I/R þ infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group. Conclusion: These results show that infliximab may protect against I/R injury in the rat I/R model.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

et al. 2012

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“…Serum and urinary creatinine was measured using the Jaffe method and calculated with the following formula: creatinine clearance ϭ (urinary creatinine ϫ 24-h urinary volume)/serum creatinine. The calculated clearance was adjusted by 100 g/rat weight (12,44).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…The participation of HO-1 in the redox imbalance in models of 30-min ischemic AKI ameliorated renal function and elevated levels of thiols and catalase, accompanied by a reduction in malondialdehyde (MDA) (44). The cytoprotective effect of heme oxygenase induction is also demonstrated in toxicity of gentamicin on NRK-52E cells by reducing apoptosis and optimizing cell viability (38).…”

mentioning

confidence: 99%

Role of Heme Oxygenase-1 in Polymyxin B-Induced Nephrotoxicity in Rats

Fonseca

Watanabe

Vattimo

2012

Antimicrob Agents Chemother

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Polymyxin B (PMB) is a cationic polypeptide antibiotic with activity against multidrug-resistant Gram-negative bacteria. PMBinduced nephrotoxicity consists of direct toxicity to the renal tubules and the release of reactive oxygen species (ROS) with oxidative damage. This study evaluated the nephroprotective effect of heme oxygenase-1 (HO-1) against PMB-induced nephrotoxicity in rats. Adult male Wistar rats, weighing 286 ؎ 12 g, were treated intraperitoneally once a day for 5 days with saline, hemin (HO-1 inducer; 10 mg/kg), zinc protoporphyrin (ZnPP) (HO-1 inhibitor; 50 mol/kg, administered before PMB on day 5), PMB (4 mg/kg), PMB plus hemin, and PMB plus ZnPP. Renal function (creatinine clearance, Jaffe method), urinary peroxides (ferrous oxidation of xylenol orange version 2 [FOX-2]), urinary thiobarbituric acid-reactive substances (TBARS), renal tissue thiols, catalase activity, and renal tissue histology were analyzed. The results showed that PMB reduced creatinine clearance (P < 0.05), with an increase in urinary peroxides and TBARS. The PMB toxicity caused a reduction in catalase activity and thiols (P < 0.05). Hemin attenuated PMB nephrotoxicity by increasing the catalase antioxidant activity (P < 0.05). The combination of PMB and ZnPP incremented the fractional interstitial area of renal tissue (P < 0.05), and acute tubular necrosis in the cortex area was also observed. This is the first study demonstrating the protective effect of HO-1 against PMB-induced nephrotoxicity.

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“…O grupo SHAM apresentou valores de função renal pelo clearence de creatinina que possibilitaram sua utilização como grupo controle para os demais, uma vez que, estiveram de acordo com os adotados em outros estudos (19,20,44) . Sendo assim, os grupos que comparados, apresentaram redução estatisticamente significante no clearance de creatinina, foram considerados como portadores de LRA (p<0,05).…”

Section: Estatísticaunclassified

“…(18) . Watanabe et al (2007) relataram que o aumento de peróxidos urinários e a diminuição das enzimas antioxidantes exerceram papel fundamental no desenvolvimento da LRA em modelo de isquemia (19) . Outros modelos de nefropatia diabética e insuficiência renal crônica também apresentam excessiva geração de EROs e consumo de enzimas antioxidantes que resultam em lesão endotelial com disfunção da hemodinâmica glomerular e lesão oxidativa das células tubulares (20,21) .…”

Section: Introductionunclassified

Efeito renoprotetor da estatina em modelo experimental de lesão renal aguda induzida por sepse

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Isoflavone and the heme oxygenase system in ischemic acute kidney injury in rats

Cited by 27 publications

References 29 publications

Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

Role of Heme Oxygenase-1 in Polymyxin B-Induced Nephrotoxicity in Rats

Efeito renoprotetor da estatina em modelo experimental de lesão renal aguda induzida por sepse

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