Isoflurane anesthesia EC50 and LC50 for zebrafish

Ku, Tien–Hsiung; Ken, Chuian-Fu; Yang, Shufen; Tsao, Yu; Chen, G. S.; Tang, Chau-Kvei

doi:10.1097/00003643-201306001-00478

Cited by 1 publication

(1 citation statement)

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“…The administration of isoflurane has been associated with accelerated apoptosis and teratological effects in mammals (mice, rats, rhesus macaque, and humans) in concentrations ranging from 32.5 to around 146 mM (Brambrink et al 2010;Istaphanous et al 2011;Davidson 2016). The information regarding the effect of these agents in zebrafish larvae is scarce; still, an effective dose of isoflurane for anesthetic purposes of 14.2 mM isoflurane has been established for 7 days postfertilization larvae while a 10 higher concentration has been defined as the mean lethal dose for this species (Ku et al 2013). More studies with isoflurane and other halogenated anesthetics are important to further assess the potential adverse effects and correspondent toxicity mechanisms of these compounds in early life forms using zebrafish pharmacological assays.…”

Section: 5: Volatile Agentsmentioning

confidence: 99%

Review on the use of zebrafish embryos to study the effects of anesthetics during early development

Félix

Coimbra

Valentim

et al. 2019

Critical Reviews in Toxicology

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Over the years, the potential toxicity of anesthetics has raised serious concerns about its safe use during pregnancy. As evidence emerged from research in animal models, showing that some anesthetic drugs are potential teratogenic, the determination of the risk of exposures to anesthetic drugs at early life stages became mandatory. However, due to inaccessibility and ethical constrains related to experimental conditions, the use of early life stages in mammalian models is limited. In this regard, some animal and nonanimal models have been suggested to surpass mammalian use in experimentation. Among them, the zebrafish embryo test has been recognized as a promising alternative in toxicology research, as well as an inexpensive and practical test. Substantial information collected from developmental research following compounds exposure, has contributed to the application of zebrafish assays in research, although only a few studies have focused on the use of early life stages of zebrafish to evaluate the developmental effects of anesthetics. Based on the recent advances of science and technology, there is a clear potential for zebrafish early life stages to provide new insights into anesthetics teratogenicity. This review provides an overview of recent anesthesia research using zebrafish embryos, demonstrating its usefulness to the anesthesia field, discussing the recent findings on various aspects related to the effects of anesthetics during early life development and the strengths and limitations of this model system.

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Section: 5: Volatile Agentsmentioning

confidence: 99%