Isolasi dan Identifikasi Bakteri Indigenous Pendegradasi Pestisida Profenofos dan Klorantraniliprol di Jombang Jawa Timur

Pratiwi, Wardha Maulidya; Asri, Mahanani Tri

doi:10.26740/lenterabio.v11n2.p300-309

Cited by 5 publications

(9 citation statements)

References 8 publications

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“…2022) found three bacterial isolates that were isolated and identified as Bacillus stearothermophilus, Bacillus badius, and Bacillus alvei, with the results of the percentage accuracy of similarity coefficients of 70%, 70%, and 60%. The three isolates of indigenous bacteria that have been identified can be used as bioremediation agents on agricultural soil contaminated with the pesticides profenofos and chlorantraniliprole 24 . Based on NGS analysis, only the species Bacillus stearothermophilus were identified in the of Bestari Probolinggo City landfill.…”

Section: Bacterial Biodiversity Results Of Next Generation Sequencing...mentioning

confidence: 99%

Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)

Qodriyah,

Sanjaya,

Wahab

et al. 2023

Valensi

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Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.

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Section: Bacterial Biodiversity Results Of Next Generation Sequencing...mentioning

confidence: 99%

Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)

Qodriyah,

Sanjaya,

Wahab

et al. 2023

Valensi

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“…Homogeneous samples are diluted to a dilution rate of 10-6 for later dissolution to be carried out on dilution of levels 10-5 and 10-6 by the pour plate method using NA media to which a 5 ppm lead solution has been added. The results of the captivity were incubated 24-48 hours at room temperature, then bacterial morphology and calculated the number of bacterial colonies in CFU / mL using the Total Plate Count (TPC) method (Pratiwi et al, 2012).…”

Section: Bacterial Isolationmentioning

confidence: 99%

Exploration and remediation ability test of indigenous bacteria from rice field pemalang regency on lead (Pb) contamined soil

Rahayu¹,

Herliana

Hadi³

et al. 2023

Proceeding ICMA-SURE

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The research aims to obtain isolates and determine the morphological characteristics of bacteria that are resistant to Lead (Pb) contamination. The materials and tools used were soil from conventional rice farming land, Nutrient Agar (NA) medium, alcohol 70%, aquades, PbNO3 solution, aluminum foil, cling wrap, heat-resistant plastic, spiritus, physiological solution of 0.85% NaCl, autoclave, test tubes, petri dishes, erlenmeyer flasks, measuring cylinder, beaker glass, laminar air flow (LAF), shaker, magnetic stirrer, micropipette, refrigerator, analytical balance, ose needle, pH meter, vortex, ice box, shovel , tube racks, bunsen, stationery, labels, and other supporting materials and equipment. This research is a sampling research and continued with laboratory tests including bacterial isolation, colony count calculation and macromorphological characterization. The data obtained from morphological observations are presented in the form of descriptions, while quantitative data are presented in the form of numbers and are used as primary data. The results of isolating bacteria with NA medium added with 5 ppm lead, there are five bacterial isolates were selected, namely KMPb O, KT1Pb C, KT2Pb H, KB1Pb H, and KB2Pb K. Based on the TPC (Total Plate Count) test with a range of 30-300 colonies, the number of bacterial colonies ranged from 10,45 x 106 – 28,9 x 106 CFU/ml. The colony morphological characteristics of the five bacterial isolates were dominated by round in shape, smooth texture and flat elevation.

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“…Uji simmons sitrat digunakan untuk mengidentifikasi kemampuan bakteri dalam menggunakan sitrat sebagai satu-satunya sumber karbon (Zulkifli et al, 2020). Uji motilitas digunakan untuk melihat apakah bakteri memiliki kemampuan bergerak atau tidak bergerak (Pratiwi & Asri, 2022). Sedangkan uji yang melibatkan glukosa, maltose, sukrosa dan laktosa bertujuan untuk mengidentifikasi kemampuan bakteri dalam memetabolisme berbagai jenis gula (Alfiansyah et al, 2023).…”

Section: Pengamatan Morfologi Koloniunclassified

Isolation and Identification of Phosphate Solubilizing Bacteria from The Rhizosphere of Dry Land Lamtoro Plants (Leucaena leucocephala) in North and South Lombok Regions

Hartanto,

Zulkifli,

Karnan

et al. 2023

JBT

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Phosphate solubilizing rhizobacteria are microorganisms that live in the rhizosphere of plants and have the ability to increase the availability of phosphate in the soil, thereby reducing dependence on chemical fertilizers which are harmful to the environment. This research aims to isolate rhizosphere bacteria from Lamtoro plants and test their ability to dissolve phosphate. Screening the ability of these bacterial isolates was carried out using Pikovskaya media and quantitatively using a spectrophotometer. The test results showed that there were 3 isolates, namely KLU13, A2 and KLS1, which had IKF values in the medium category. Meanwhile, the other 4 isolates had abilities in the low category (isolates A1, A31BK, A3BB and A3). The results of quantitative tests with a spectrophotometer showed that there was an increase in dissolved phosphate from day 2 to day 6. Isolates KLU13, A3 and KLS1 dissolved the highest phosphate on day 6 with values of 14.65 ppm, 14.81 ppm and 10.32 ppm respectively. It can be concluded that the isolate obtained from the Lamtoro rhizosphere which grows in the dry areas of Lombok Island has the potential to be developed as a biofertilizer in the future.

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Isolasi dan Identifikasi Bakteri Indigenous Pendegradasi Pestisida Profenofos dan Klorantraniliprol di Jombang Jawa Timur

Cited by 5 publications

References 8 publications

Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)

Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)

Exploration and remediation ability test of indigenous bacteria from rice field pemalang regency on lead (Pb) contamined soil

Isolation and Identification of Phosphate Solubilizing Bacteria from The Rhizosphere of Dry Land Lamtoro Plants (Leucaena leucocephala) in North and South Lombok Regions

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