Isolasi dan identifikasi Staphylococcus aureus dari susu mastitis subklinis di Tasikmalaya, Jawa Barat

Herlina, Nina; Afiati, Fifi; Cahyo, Aditia Dwi; Herdiyani, Poppy Dwie; Qurotunnada, Qurotunnada; Tappa, Baharuddin

doi:10.13057/psnmbi/m010305

Cited by 14 publications

(13 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hemolysis zone was formed due to the presence of hemolysin toxins produced by S. aureus. 19 Hemolysin is an exoprotein that has enzymatic activity and toxins. 20 S. aureus bacteria have four types of hemolysin.…”

Section: 2mentioning

confidence: 99%

The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

Turista

Puspitasari

2019

J. Teknol. Lab

View full text Add to dashboard Cite

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

show abstract

Section: 2mentioning

confidence: 99%

The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

Turista

Puspitasari

2019

J. Teknol. Lab

View full text Add to dashboard Cite

show abstract

“…Nilai CMT berhubungan dengan jumlah sel somatik yang terkandung dalam susu. Semakin tinggi nilai CMT, semakin tinggi jumlah sel somatik yang ditunjukan dengan viskosistas reaksi yang semakin tinggi (Herlina et al 2015). Jumlah sel somatik tersebut dijadikan sebagai acuan untuk menjustifikasi sapi yang terinfeksi mastitis subklinis.…”

Section: Hasil Uji Cmtunclassified

Ekspresi dan Pathway Analisis Gen CD14 dan IL10 pada Sapi Perah yang Terinfeksi Mastitis Subklinis

Widiono¹,

Gunawan²,

Sumantri³

et al. 2019

JIPTHP

View full text Add to dashboard Cite

Mastitis is an inflammation of internal udder tissue caused by bacteria, it decrease milk production both in quality and quantity. This study was aimed to identify expression and pathway analysis of gene CD14 and IL10 as resistance genes in dairy infected with subclinical mastitis. Sample were taken from milk and blood 10 healthy dairy and 10 dairy containing subclinical mastitis used for CMT and RNA isolation used for gene expression analysis. The result showed that expression in healthy dairy CD14 gene had a higher expression (P<0.05). In contrast the IL10 gene had a high expression in infected dairy with subclinical mastitis (P<0.05). CD14 was an early signal receptor that activates a complex nonspecific immune response to induce various immune characteristics such as the production of inflammatory cytokines and chemokines, the production of antimicrobial peptides and so on destroy pathogens. The high expression of IL10 in infected dairy with subclinical mastitis suggested that immune responses were not functioning properly considered due to inhibiton of cytokine transduction signals by excessive production of IL10.

show abstract

“…dan E. coli dan sesuai dengan Standar Nasional Indonesia (SNI) kecap ikan (BSN 1996). Staphylococcus merupakan salah satu bakteri patogen yang dapat menyebabkan berbagai jenis infeksi mulai dari infeksi kulit ringan hingga keracunan makanan, sehingga sangat penting untuk mengetahui keberadaan bakteri tersebut pada suatu produk makanan (Herlina et al 2015).…”

Section: Cemaran Mikrobaunclassified

Microbiologys Quality of Fish Sauce Tongkol (Euthynnus affinis) with Additions Pineapple Juice (Ananas comosus)

Siahaan¹,

Dien²,

Onibala³

2018

Jurnal PHPI

View full text Add to dashboard Cite

AbstrakKecap ikan merupakan salah satu produk perikanan dari proses fermentasi. Ikan yang digunakan dalam penelitian ini yaitu ikan tongkol (Euthynnus affinis) yang merupakan komoditas tangkapan tertinggi di Sulawesi Utara. Penelitian ini bertujuan untuk menentukan mutu mikrobiologi dari produk diversifikasi ikan tongkol menjadi kecap ikan yang aman untuk dikonsumsi. Penelitian ini menggunakan metode ekperimental. Penelitian ini menggunakan penambahan (A) 0%, (B) 9%, (C) 12%, (D) 15% sari buah nanas dan garam (G8) 8%, (G9) 9%, (G10)10% garam, dan penyimpanan didalam inkubator selama 6, 8, 10 hari. Variabel yang diamati adalah total lactic acid bacteria, Total mikroba, E. coli, Staphylococcus sp., pewarnaan gram, pH, kadar air dan uji organoleptik. Hasil penelitian menunjukkan bahwa sampel D (D1G10) dengan penambahan sari buah nanas 15 % menyebabkan penurunan jumlah total bakteri dan menyebabkan meningkatnya jumlah total bakteri asam laktat. Total lactic acid bacteria, Total mikroba, E. coli, Staphylococcus sp., pewarnaan gram, pH, kadar air dan uji organoleptik dengan penambahan sari buah nanas 15% masing-masing 1,0 x 10 2 ; 9,0 x 10 4 ; 5,46; 69,14%; negatif; 100 isolat positif batang; kenampakan 8; bau 8; dan rasa 8. Hasil tersebut sudah memebuhi standar nasional Indonesia (SNI) dan aman untuk dikonsumsi.Kata kunci: bakteri asam laktat, cemaran mikroba, fermentasi Microbiologys Quality of Fish Sauce Tongkol (Euthynnus affinis) with Additions Pineapple Juice (Ananas comosus)Abstract Fish sauce is one of fish fishery product from fermentation process. In this study fish sauce made from tongkol fish (Euthynnus affinis) that has high catching in North Sulawesi. This study aimed to make diversification product from Tongkol fish into fish sauce safe to eat. The research method applied was experimental laboratories. In this research was by additions (A) 0%, (B) 9%, (C) 12%, (D) 15% pineapple juice and (G8) 8%, (G9) 9%, (G10) 10% salt and stored in an incubator at temperature 50ºC during 6, 8, 10 days. Variable measured were total lactic acid bacteria, total plate count, E. coli, Staphylococcus sp., gram strain, pH, water consentration and organoleptic test. The result showed that sample D (D1G10) with additions pineapple juice 15% caused decrease total pathogen bacteria and caused increase lactic acid bacteria. Showed that total lactic acid bacteria, total plate count, E. coli, Staphylococcus sp., gram strain, pH, water consentration, gram strain, organoleptic test with pineapple juice 15% was 1.0x10 2 ; 9.0x10 4 ; 5.46; 69.14%; negative; 100 isolate positive shaped rod; the appearance 8; smell 8; taste 8. The result accordance with Standard National Indonesia (SNI) and safe to eat.

show abstract

Isolasi dan identifikasi Staphylococcus aureus dari susu mastitis subklinis di Tasikmalaya, Jawa Barat

Cited by 14 publications

References 3 publications

The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

Ekspresi dan Pathway Analisis Gen CD14 dan IL10 pada Sapi Perah yang Terinfeksi Mastitis Subklinis

Microbiologys Quality of Fish Sauce Tongkol (Euthynnus affinis) with Additions Pineapple Juice (Ananas comosus)

Contact Info

Product

Resources

About