Isolasi dan karakterisasi Bakteri Asam Laktat (BAL) dari Ale-ale dan Cincalok

Nurhamidah, Anggia; Warsidah, Warsidah; Idiawati, Nora

doi:10.26418/lkuntan.v2i3.34780

Cited by 6 publications

(6 citation statements)

References 1 publication

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“…A catalase test was carried out to determine the ability to produce catalase enzyme and isolate tolerance to oxygen. Catalase is an enzyme capable of catalyzing the direct conversion of hydrogen peroxide (H2O2), which is toxic to cells, into water and oxygen [17]. The chemical reaction produced by the catalysis of the enzyme catalase against H2O2 is: catalase 2 H2O2…”

Section: Catalase Testmentioning

confidence: 99%

Isolation and Identification of Lactic Acid Bacteria of Bekamal (Banyuwangi Traditional Fermented Meat)

Prastujati

Hilmi

Khusna

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Fermentation technology has been used for decades for dairy and meat products. Several studies have shown an increase in the nutritional value of fermented meat and can increase its safety and shelf life. Bekamal is a traditional fermented meat product found in Banyuwangi Regency. Lactic acid bacteria is one of the natural microbiota of meat that is widely used as a fermentation agent to maintain quality and improve the product’s hygiene and sensory properties. This study aimed to indetify lactic acid bacteria (LAB) from traditional fermented meat products, namely bekamal. Bekamal tested is meat that has undergone a fermentation process for 7, 14 and 21 days, and each fermentation time consists of one sample to be isolated and identified the presence of lactic acid bacteria in it. The results showed that in bekamal, some lactic acid bacteria were indicated by Gram staining results, namely gram-positive and catalase-positive, and the results of the CO2 production test were classified as heterofermentative bacteria. This research concluded that in bekamal, lactic acid bacteria were indicated from Lactobacillus, Streptococcus and Leuconostoc genus.

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Section: Catalase Testmentioning

confidence: 99%

Isolation and Identification of Lactic Acid Bacteria of Bekamal (Banyuwangi Traditional Fermented Meat)

Prastujati

Hilmi

Khusna

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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show abstract

“…This indicated the bacterial isolates could break down H 2 O 2 and produce oxygen gas. Based on Nurhamidah et al 12 , positive catalase is indicated by the presence of air bubbles in the isolate, indicating the presence of the catalase enzyme produced by bacteria that can convert hydrogen peroxide into water and oxygen. According to Holt et al 13 BAL is catalase negative.…”

Section: Bacterial Physiologymentioning

confidence: 99%

“…Some bacteria ferment glucose and produce various acidic products that will lower the pH of the growth medium to 5 or lower. Adding a pH indicator methyl red can show a change in pH to acid 12 .…”

Section: Bacterial Physiologymentioning

confidence: 99%

ISOLATION OF LACTIC ACID BACTERIA FROM VANAME SHRIMP (Litopenaeus vannamei) AND ITS ANTAGONISM TO PATHOGEN BACTERIA

Nur Sufaidah,

Nursyirwani,

Feliatra

2024

ajoas

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Vaname shrimp is one of Indonesia's leading export species and is widely cultivated. The main problem in vaname shrimp pond cultivation is the attack of shrimp diseases caused by bacteria or viruses. This study aims to isolate and identify lactic acid bacteria (BAL) from vaname shrimp (Litopenaeus vannamei) and test their antagonism against pathogenic bacteria. The research was conducted from February to April 2023. The method used in this study is a survey method. Sampling was conducted at the vaname shrimp pond of the Fisheries Service Task Unit (UPT), Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The method used in this research was a survey method. BAL was isolated using MRS Agar + CaCO3 media. The Kirby-Bauer method was used to test bacterial antagonism against pathogenic bacteria. Based on the identification of seven isolates, it was found that only four isolates were positively identified as LAB bacteria, namely 47D, 54T1, 73DB1, and 73BD2. The results of the antagonism test against pathogenic bacteria showed that all BAL isolates could not suppress the growth of E. coli bacteria. However, these isolates suppressed the growth of A. hydrophila bacteria, with the widest inhibition zone diameter on isolate 54T1 (4.2 mm). On Vibrio sp. bacteria, the widest inhibition zone of BAL was on isolate 73DB1 (6 mm). These results indicate that the antibacterial activity produced by BAL is still relatively weak

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“…The film (Figure 2c) has similar characteristics to fragments, irregular, but its color is transparent. The type of fiber (Figure 2d) is a type of microplastic such as thin fibers that easily accumulate in sediments [7]. Based on the Figure 3, the fragment type is known to have the highest average abundance both in waters (4,4 × 10 6 particles/km 2 ) and sediments (7,6 × 10 8 partikel/km 2 ).…”

Section: Types Of Microplasctics Found In Surabaya Mangrove Areasmentioning

confidence: 99%

Microplastic abundance in Surabaya mangrove areas during the wet season

Nafisyah,

Iswandi,

Karisma

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Plastic waste pollution is the most dominant problem in various parts of the world, and it has increased since 2020. Degraded plastic waste can accumulate in the environment, especially the mangrove ecosystem, which is a sediment trapper, allowing for the accumulation of various materials. This study was done during the wet season in December 2021 at Wonorejo mangrove areas, Surabaya, Indonesia. The aims of study were to observe the abundance of microplastic that accumulates not only in waters during high tide but also in sediments during low tide. The results will provide information about environmental pollution of the Surabaya mangrove ecosystem. Four types of microplastics were observed in water and sediment samples under the stereo microscope, i.e. fragments, fibers, films, and granules. The average abundance of microplastics in waters from five stations was 8×106 particles km-2, while in sediments (three stations) was counted as 1.5×109 particles km-2 on average. The Fourier Transform Infra-Red (FTIR) analysis was done resulting in the characteristic of microplastics. From both waters and sediments, contamination of microplastics consisted of Polyvinyl alcohol (PVA), Polyvinyl chloride (PVC), Polypropylene (PP), Polystyrene (PS), and Polyethylene (PE).

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Isolasi dan karakterisasi Bakteri Asam Laktat (BAL) dari Ale-ale dan Cincalok

Cited by 6 publications

References 1 publication

Isolation and Identification of Lactic Acid Bacteria of Bekamal (Banyuwangi Traditional Fermented Meat)

Isolation and Identification of Lactic Acid Bacteria of Bekamal (Banyuwangi Traditional Fermented Meat)

ISOLATION OF LACTIC ACID BACTERIA FROM VANAME SHRIMP (Litopenaeus vannamei) AND ITS ANTAGONISM TO PATHOGEN BACTERIA

Microplastic abundance in Surabaya mangrove areas during the wet season

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