Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China

Desriani, Desriani; Safira, Ukhradia Maharaniq; Bintang, Maria; Rivai, Akhmad; Lisdiyanti, Puspita

doi:10.25077/jka.v3i2.33

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…Usually, endophytic bacteria are abundant in roots and descend on stems and leaves. This can be caused by roots that are in the soil so that soil bacteria can be found and most endophytic bacteria enter through the soiled tissue so that the number of roots is more in the roots [12]. But sometimes the highest number of endophytic bacteria is in the stem.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Cellulase- Producing Encophytic Bacteria From Yellow Root Plants (Arvangelisia flava (L.) Merr) From Enggano Island

Reformanda¹,

Sipriyadi²,

Darwis³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Endophytic bacteria are bacteria that live in plant tissue without causing disease in their host. In general, endophytic bacteria enter through stomata or wounds in plants by producing cellulase enzymes to degrade cellulose in plant cell walls that contain cellulose, one of which is yellow root (Arcangelisia flava (L.) Merr). The purpose of this study was to isolate endophytic bacteria from yellow roots from Enggano Island, Bengkulu Province, and to identify morphologically, Gram staining and biochemical tests as well as testing the potential of endophytic bacteria in producing cellulase enzymes. Isolation was carried out by the patch method with surface sterilization, using 70% alcohol and 5.25% sodium hypochlorite and then put on NA media that had been treated with Nystatin. The isolates obtained were then tested for their ability to produce cellulase enzymes by scratching them onto CMC media, then measuring the forming of clear zone. The results showed that from 29 isolates, 26 of them were able to degrade cellulose. AKEBG26 and AKEBG25 isolates had a higher ability to hydrolyze cellulose with cellolulitic potential index (IP) were about ±2.90 and ±1.51. Identification based on gram staining and biochemical tests of 8 endophytic bacterial isolates that had the potential to produce cellulase were closely related to 3 genera, namely Bacillus, Amphibacillus, and Micrococcus.

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Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Cellulase- Producing Encophytic Bacteria From Yellow Root Plants (Arvangelisia flava (L.) Merr) From Enggano Island

Reformanda¹,

Sipriyadi²,

Darwis³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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“…[13] yang ada dijaringan tanaman inangnya [14], baik berupa jamur dan bakteri yang hidup dalam jaringan tanaman pada periode tertentu dengan membentuk koloni dalam jaringan tanaman tanpa membahayakan inangnya [15] dan terutama kan dapat menghasilkan senyawa bioaktif yang memiliki karakternya hampir sama dengan tumbuhan inangnya [16]. Penelitian isolatif terhadap bakteri endofit pada berbagai tanaman sudah banyak dilaporkan, antara lain dari spons karang [17], kunyit [18], ketepeng cina [19], yang menyatakan bahwa kemampuan bakteri endofit yang dihasilkan sama dengan kemampuan inangnya. [20].…”

Section: Pendahuluanunclassified

POTENSI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI BAKTERI ENDOFIT PADA DAUN Moringa oleifera L

Yati

Sumpono

Candra

2018

atp

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[POTENTIAL ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITES FROM ENDOPHYTE BACTERIA ON Moringa oleifera L LEAF] This research aims to know the similarities between secondary compound metabolites produced by endophyte bacterial on host leaves and from Moringa oleifera L (kelor) leaves as well as determine the magnitude of antioxidant activity compounds of secondary metabolites produced by bacterial endophyte. Based on the results of the test compound is secondary metabolite in both samples, for secondary metabolite compounds in the extract of the leaves of M. oleifera L. contains identified steroid compounds, flavonoids, saponins, tannins and phenolic compounds, while for metabolites secondary bacterial endophyte undetectable on the existence of any secondary metabolite compounds.For testing of antioxidants on endophyte bacterial extracts of leaves of M. oleifera L is done using the DPPH method, performed on variations of the concentration of 200, 400, 600, 800, and 1000 ppm with Ascorbic acid as a comparison. The results of the test get an IC50 of M. oleifera L leaf extract at 243.67 ppm, and supernatan extract from endophyte bacterial at 492 ppm. The IC50 values showed antioxidant activity in secondary metabolite compounds derived from extracts of the leaves of M. oleifera L which has an extreme antioxidant category. The amount of antioxidant activity for secondary metabolite compound extracts of bacterial endophyte on leaves of M. oleifera L category is feeble, and to extract the supernatan activity is weak compared to the value of the antioxidant activity of Ascorbic acid.

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“…Beberapa mikroba endofit dari kelompok bakteri memiliki aktivitas antagonis yang ditandai dengan terbentuknya zona hambat, hal ini mengindikasikan bakteri tersebut. (Desriani et al, 2014).…”

Section: Pendahuluanunclassified

Uji Antagonis Pseudomonas sp. Asal Endofit Perakaran Padi Terhadap Penyakit Blas (Pyricularia oryzae) Secara In Vitro

Saylendra¹,

Rusbana²,

Herdiani³

2018

Agrologia

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The purpose of this research was to determine the antagonistic bacteria Pseudomonas spp. of paddy roots endophytic against disease blast (Pycularia oryzae) in vitro. This research was conducted at the Laboratory of Agroecology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa on April to June 2015. This research implemented experimental one factor that arranged in Completely Randomized Design with three replications. The treatment consisted of 9 isolates of Pseudomonas sp. which was screened from endophytic roots of paddy plants. The test results showed that antagonistic in vitro testing could inhibit the growth P. oryzae. Inhibition of 9 isolates to the growth Pseudomonas spp. had the significant clear zone. Isolate Ps 17 (58.89%), Ps 22 (54.44%), Ps 37 (52.06%) and Ps 39 (61.42%) have the largest inhibition. The results showed that the bacteria Pseudomonas sp. on this research is potential on controlling disease blast in paddy plant.

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Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China

Cited by 13 publications

References 11 publications

Isolation and Identification of Cellulase- Producing Encophytic Bacteria From Yellow Root Plants (Arvangelisia flava (L.) Merr) From Enggano Island

Isolation and Identification of Cellulase- Producing Encophytic Bacteria From Yellow Root Plants (Arvangelisia flava (L.) Merr) From Enggano Island

POTENSI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI BAKTERI ENDOFIT PADA DAUN Moringa oleifera L

Uji Antagonis Pseudomonas sp. Asal Endofit Perakaran Padi Terhadap Penyakit Blas (Pyricularia oryzae) Secara In Vitro

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