Isolasi dan Seleksi Bakteri Proteolitik Asal Limbah Cair Tahu Sebagai Dasar Penentuan Agen Pembuatan Biofertilizer

Asril, Muhammad; Leksikowati, Sovia Santi

doi:10.22373/ekw.v5i2.4356

Cited by 6 publications

(8 citation statements)

References 10 publications

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“…The clear area is an indicator that isolates can utilize protein in the media as a nutritional source. The protease index produced by Candida tropicalis is smaller compared to the protease index produced by isolates from tofu wastewater (Asril & Leksikowati, 2019).…”

Section: Discussionmentioning

confidence: 77%

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Puspadewi

Adirestuti

Dewi

et al. 2020

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Soil microorganisms have one function as a source of producing extracellular enzymes, especially the lipase and protease enzymes used to catalysis a chemical process. Protease is one of the enzymes with the best prospects for development because it is quite widely applied in various industries, both food and non-food. Most of the lipases used in the food and pharmaceutical industries come from microorganisms. This study aimed to look at the extracellular enzyme activity of Candida tropicalis isolated from the soil of the medicinal plantations of the Faculty of Pharmacy Unjani. The study was conducted through a fermentation process to obtain bioactive metabolites of isolates. The result was analyzed using ANOVA within a 5% interval of confidence, continuing with PostHoc. Based on these results, the Candida tropicalis metabolite giving the best proteolytic index value (0,6556 ± 0,0090) U/mL. The results showed that the metabolite isolate of Candida tropicalis had the highest activity, amounting to 5,776 ± 0,495 U/mL. The best results of lipolytic index value (0,394 ± 0,053) U/mL. The Candida tropicalis metabolites produced the highest lipase enzyme after nine days of fermentation in 5.2917±0,0167 U/mL.

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Section: Discussionmentioning

confidence: 77%

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Puspadewi

Adirestuti

Dewi

et al. 2020

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“…Isolat bakteri yang memiliki indeks pelarut fosfat tertinggi dilakukan uji patogenitas sederhana pada umbi kentang yang telah dimodifikasi (12) . Metode uji patogenitas ini merupakan modifikasi dari Lelliot dan Stead (13) .…”

Section: Pengujian Patogenitas Isolat Bakteri Potensialunclassified

“…Umbi kentang menjadi salah satu sampel model untuk pengujian degradasi pektin yang biasanya ditemukan pada dinding sel tanaman. Indikasi bakteri digolongkan sebagai patogen tanaman yaitu terjadinya lesi pada kentang yang diujikan (12) . Penggunaan kentang sebagai inang model dikarenakan kentang merupakan inang alternatif yang mampu memberikan informasi terhadap patogenitas suatu bakteri uji (32) .…”

Section: Patogenitas Isolat Potensialunclassified

Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera

Asril

Lisafitri

2020

Jurtekling

Self Cite

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Phosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam

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“…Research aimed at exploring proteolytic bacteria has been carried out by calculating the Proteolytic Index (IP), including derived from peat soils (Mahdiyah, 2015), mangrove forest land (Hastuti Sri et al, 2017), Tofu liquid waste (Asril &;Leksikowati, 2019), and on a 72-hour postfermentation red oncom (Lestari et al, 2018). From several studies, potential proteolytic bacteria with varying IP values were obtained.…”

Section: Introductionmentioning

confidence: 99%

“…From several studies, potential proteolytic bacteria with varying IP values were obtained. Five isolates derived from peat soils have proteolytic capabilities with IP 3 (Mahdiyah, 2015), from mangrove forest soil 1.62-2.94 and one of them was identified from the species Vibrio parahaemolyticus (Hastuti Sri et al, 2017), from tofu liquid waste 3.20 (Asril &;Leksikowati, 2019). The formation of a clear zone with a diameter of 78 mm around the bacterial colony showed that protease activity of bacterial isolates from red oncom samples after 72 hours of fermentation (Lestari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Selection of Proteolytic Bacteria from Bonoloyo Public Burial Place (PBP), Banjarsari, Surakarta, Central Java

Putri Salwa Salsabilla,

Yasir Sidiq,

Erma Musbita Tyastuti

et al. 2024

Quagga

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Public Burial Place (PBP) is a cemetery for everyone. In PBP, protein decomposition occurs actively because the human body is made up of 16.4% protein, so it is most likely that proteolytic bacteria can be found. Previous research has isolated as many as 45 isolates from PBP Bonoloyo, Surakarta, but has not selected their proteolytic capabilities. The aim of the study was to isolate proteolytic bacteria from the Bonoloyo Public Cemetery and identify them. The bacterial isolate of PBP Bonoloyo is a collection from the FKIP Biological Laboratory of Muhammadiyah University of Surakarta that selected its proteolytic capabilities using the milk agar scheme (SMA). The parameters measured are the diameter of the colony and the lymphatic zone formed to obtain the value of the proteolytic index (IP). For bacterial isolates showing a positive proteolytic activity, simple identification is carried out through the observation of colonial morphology and Gramme colouring. The results of the study showed that as many as 28 isolates (62.2%) were positive for proteolytic activity, with IP values >2 for B1 and B3. Bacterial proteolytic isolates are dominantly white to yellow with irregular, filamentary, spindle, and circular colonial forms. The results of Gramme colouring show that bacterial isolates that have proteolytic abilities are a group of Gramme negative bacteria that are basil shaped. The bacterial isolate that shows the highest IP value is the B1 isolate, which is suspected to be of the genus Bacillus. This research obtained data showing that in the public cemetery (PBP), Bonoloyo stores potential as a proteolytic bacterial habitat for a group of Gramme negative bacteria.

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Isolasi dan Seleksi Bakteri Proteolitik Asal Limbah Cair Tahu Sebagai Dasar Penentuan Agen Pembuatan Biofertilizer

Cited by 6 publications

References 10 publications

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera

Selection of Proteolytic Bacteria from Bonoloyo Public Burial Place (PBP), Banjarsari, Surakarta, Central Java

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