ISOLASI DAN UJI POTENSI BAKTERI PELARUT FOSFAT DARI LAHAN PERKEBUNAN KELAPA SAWIT (Elaeis guineensis Jacq.)

Abstract

Phosphate solubilizing bacteria has high potential in dissolving P bound into P available in the soil, the mechanism of dissolving P related to the activity of the microbes concerned in producing enzymes and organic acids. This research aims were to determine the type and ability of bacteria that can dissolve P. This research was conducted at the Soil Biology Laboratory, Faculty of Agriculture, University of Sumatera Utara (USU) in January-June 2019. The observed parameters were the population of phosphate solubilizing microorganisms, potential test on solid media from several P sources, analysis of organic acids using the High-Performance Liquid Chromatography (HPLC) method, identification of bacteria by Polymerase Chain Reaction Sequences (PCR-Sequence). The analysis results indicated that the microbes which had the highest ability to dissolve P and produce organic acids were isolates with B1 code and the results of PCR-Sequence identification of B1 isolates were Burkholderia sp strain IBP-VNS5.