Isolasi, Karakterisasi, dan Identifikasi Bakteri Patogen pada Tumbuhan Kantong Semar (Nepenthes gracillis)

Prastio, Rahmat Agus; Isnawati, Isnawati; Rahayu, Dwi Anggorowati

doi:10.26740/lenterabio.v11n2.p255-262

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…SDM II B05, LBR I A03, SWR II B04 and SWR I A02 were classified as gram-negative and had endotoxins in their cell walls, suggesting that they could inhibit the growth of F. oxysporum. This is consistent with the result of Prasetya et al (2018), reporting that bacteria isolates AA2, AA8, AA9 and AA10 were gram-negative and produced chitinase enzymes to inhibit the growth of F. oxysporum, compared to AA7 bacteria which was gram-positive and could not inhibit fungi growth.…”

Section: Endophytic Bacteria Inhibitionsupporting

confidence: 92%

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Nurlila¹,

Fua

Abidin³

et al. 2022

Caraka Tani J. Sustain. Agric.

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Fusarium wilt is a plant disease caused by Fusarium oxysporum that generates significant economic loss to crops. A method to sustainably control F. oxysporum is utilizing biological agents, such as endophytic bacteria. Therefore, this study aimed to isolate endophytic bacteria from tomato plant tissue, which could inhibit the pathogen of wilt disease (F. oxysporum). Endophytic bacteria were isolated from local tomato plants in Muna Regency, Indonesia. Morphological characteristics such as size, shape, color and height of bacterial colonies were then determined by Gram staining using potassium hydroxide (KOH). Endophytic bacterial isolates were evaluated for their ability to inhibit F. oxysporum through inhibition and hydrogen cyanide (HCN) production tests. Subsequently, analysis of variance was used to determine whether endophytic bacteria inhibited F. oxysporum growth, and if there was a significant effect, Duncan's test was conducted at 95% significance. HCN production was observed through qualitative methods. The results showed that four endophytic bacteria isolates, namely LBR I A03, SWR II B04, SDM II B05 and SWR I A02 inhibited the growth of F. oxysporum by more than 50%. It also revealed that four endophytic bacterial isolates were strong HCN producers and two were weak producers. Therefore, isolates showing antifungal activity in this study can be used as biopesticide agents to induce plant resistance to F. oxysporum.

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Section: Endophytic Bacteria Inhibitionsupporting

confidence: 92%

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Nurlila¹,

Fua

Abidin³

et al. 2022

Caraka Tani J. Sustain. Agric.

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“…Uji fisiologi biasanya identik dengan uji biokimia (Prastio et al, 2022). Bakteri diidentifikasi melalui uji fisiologi berdasarkan aktivitas selnya.…”

Section: Pendahuluanunclassified

Karakterisasi Morfologi Dan Fisiologi Mikrob Pelarut Fosfat Dan Mikrob Penambat N2 (Azotobacter) Dari Rhizosfer Tanaman Padi Dan Tanah Hutan Cifor Dramaga Bogor

Maatoke,

Dewani,

Suciati

2024

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This study aims to determine the morphological and physiological properties of Phosphate Solubilizing Microbes (MPF) and Azotobacter. The method used was to test morphological properties using gram staining and physiological properties tests using biochemical tests based on cell activity by looking at the interaction of bacterial metabolism with the test reagent media. Data analysis examined the reaction to the isolated morphological and physiological test media. The results showed that based on the morphological observations of the MPF, the colonies showed a slightly milky white color, round, convex, slimy shape, 1-2 mm in diameter and flattened, and Gram-positive. Azotobacter shows the characteristics of transparent color, convex, flat edges, slightly slimy, an average diameter of 1 mm, and is Gram-negative; whereas based on the results of physiological test observations for catalase and oxidation tests, the characteristic of MPF and Azotobacter, each showed a positive (+) effect, as seen from the formation of bubbles in the test results. Tests for motility and O2 requirements show that both bacteria are motile, mobile and aerobic or require oxygen for growth. The observations of resistance in the pH range showed that Azotobacter was more resistant to extreme acid-base pH with a pH range of 3-9, while MPF could only survive in a pH range of 5-9. Tests of glucose and sucrose fermentation showed that MPF did not form gas bubbles (-) and no color change(-). At the same time, Azotobacter showed the formation of gas bubbles (+) but no color change (-).

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“…Identifikasi bakteri terdiri dari dua metode, yaitu secara fenotipik dan secara genomik. Identifikasi bakteri secara fenotipik adalah metode identifikasi secara konvensional berdasarkan pengamatan terhadap morfologi sel, pewarnaan gram, uji oksidasi, uji biokimia, uji fisiologi, dan uji fermentasi (Prastio et al, 2022). Sedangkan, identifikasi bakteri secara genotipik merupakan identifikasi menggunakan metode molekuler berdasarkan urutan nukleotida gen 16S rRNA dengan metode sekuensing dan pembuatan pohon filogenetik (Nuritasari et al, 2017).…”

Section: Pendahuluanunclassified

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

Maulida,

Lisdiana

2024

LenteraBio

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Bakteri merupakan salah satu agen bioremediasi untuk mengatasi pencemaran di lahan pertanian. Bakteri memiliki kemampuan mendegradasi senyawa kontaminan. Isolat bakteri CF6 merupakan isolat bakteri rhizosfer dari tanah pertanian cabai rawit yang berpotensi mampu mendegradasi herbisida glifosat. Namun, perlu diidentifikasi untuk diaplikasikan sebagai agen bioremediasi dan dipahami pengaruhnya terhadap ekosistem. Tujuan penelitian ini adalah mengidentifikasi secara fenotipik dan genomik isolat bakteri rhizosfer yang berpotensi sebagai pendegradasi herbisida glifosat sehingga dapat diaplikasikan lebih lanjut. Metode identifikasi secara fenotipik terdiri dari uji motilitas, pewarnaan gram, katalase, sitrat, urease, endospora, Voges-Proskauer, produksi indol, methyl red, pertumbuhan pada 5% NaCl, dan uji reduksi gula. Metode identifikasi secara genomik terbagi empat tahap, yaitu isolasi DNA, amplifikasi gen 16S rRNA, elektroforesis, dan sekuensing gen 16S rRNA. Analisis data dari hasil uji fenotipik secara numerik menggunakan NTSys 2.11a dan hasil uji genomik menggunakan MEGA11. Hasil dendrogram dalam penelitian ini menunjukkan isolat bakteri CF6 termasuk dalam satu klad dengan bakteri-bakteri anggota genus Klebsiella. Sedangkan hasil analisis BLASTn menunjukkan isolat uji CF6 memiliki kesamaan sekuen gen 16S rRNA dengan Klebsiella quasipneumoniae sebesar 98,62%. Dari hasil kedua metode identifikasi tersebut dapat disimpulkan bahwa isolat bakteri CF6 adalah bakteri K. quasipneumoniae. Aspek patogenitas dan laju degradasi bakteri tersebut perlu diuji sebelum diimplementasikan.

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Isolasi, Karakterisasi, dan Identifikasi Bakteri Patogen pada Tumbuhan Kantong Semar (Nepenthes gracillis)

Cited by 4 publications

References 7 publications

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Karakterisasi Morfologi Dan Fisiologi Mikrob Pelarut Fosfat Dan Mikrob Penambat N2 (Azotobacter) Dari Rhizosfer Tanaman Padi Dan Tanah Hutan Cifor Dramaga Bogor

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

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