Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella

Hashizume, Tomomi; Momoi, Fumiki; Kurita‐Ochiai, Tomoko; Kaminogawa, Shuichi; Hosono, Akira; Kataoka, Kosuke; Shinozaki-Kuwahara, Noriko; Kweon, Mi‐Na; Yamamoto, Masafumi

doi:10.1016/j.bbrc.2007.06.096

Cited by 9 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S1C and Movie S2), which resemble PPs and still develop in PP-null mice (19). This result was identical to previous reports showing that PPs are the major sites for uptake of orally inoculated bacteria and the subsequent induction of host immune responses (e.g., Salmonella typhimurium and Helicobacter pylori) (20,21).…”

Section: Resultssupporting

confidence: 89%

Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis

Obata

Goto

Kunisawa

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

202

185

View full text Add to dashboard Cite

The indigenous bacteria create natural cohabitation niches together with mucosal Abs in the gastrointestinal (GI) tract. Here we report that opportunistic bacteria, largely Alcaligenes species, specifically inhabit host Peyer's patches (PPs) and isolated lymphoid follicles, with the associated preferential induction of antigen-specific mucosal IgA Abs in the GI tract. Alcaligenes were identified as the dominant bacteria on the interior of PPs from naïve, specific-pathogen-free but not from germ-free mice. Oral transfer of intratissue uncultured Alcaligenes into germ-free mice resulted in the presence of Alcaligenes inside the PPs of recipients. This result was further supported by the induction of antigen-specific Ab-producing cells in the mucosal (e.g., PPs) but not systemic compartment (e.g., spleen). The preferential presence of Alcaligenes inside PPs and the associated induction of intestinal secretory IgA Abs were also observed in both monkeys and humans. Localized mucosal Ab-mediated symbiotic immune responses were supported by Alcaligenes-stimulated CD11c + dendritic cells (DCs) producing the Ab-enhancing cytokines TGF-β, B-cell-activating factor belonging to the TNF family, and IL-6 in PPs. These CD11c + DCs did not migrate beyond the draining mesenteric lymph nodes. In the absence of antigen-specific mucosal Abs, the presence of Alcaligenes in PPs was greatly diminished. Thus, indigenous opportunistic bacteria uniquely inhabit PPs, leading to PP-DCsinitiated, local antigen-specific Ab production; this may involve the creation of an optimal symbiotic environment on the interior of the PPs.Alcaligenes | intratissue habitation | Peyer's patch

show abstract

Section: Resultssupporting

confidence: 89%

Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis

Obata

Goto

Kunisawa

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

202

185

View full text Add to dashboard Cite

show abstract

“…Furthermore, the structure of this small lymphoid follicle resembles that of PP (8,20). These findings imply that ILF could be an IgA inductive site for the induction of Ag-specific intestinal IgA Ab responses after oral immunization with rSalmonella-Tox C. However, despite the presence of ILF in PP-null mice (8,9,20,22), the PP-null mice in the present study failed to induce Ag-specific mucosal IgA Ab responses following oral immunization with rSalmonella-Tox C. In addition, Salmonella LPS-specific IgA Ab responses were not detected in the intestines of PP-null mice, while ILF-null mice induced mucosal IgA anti-LPS Ab responses when rSalmonella-Tox C was given orally. These results suggest that ILF do not compensate for the function of PP in the induction of intestinal IgA Ab responses when rSalmonella-Tox C is given .…”

Section: Discussionmentioning

confidence: 89%

Peyer's Patches Are Required for Intestinal Immunoglobulin A Responses toSalmonellaspp

Hashizume

Togawa²,

Nochi

et al. 2008

Infect Immun

Self Cite

View full text Add to dashboard Cite

“…Therefore, the exclusive role of PP for the induction of IgA has been a matter of debate for a long time. Recent observations indicate that PP appear to be essential for intestinal immune responses to Salmonella (45)(46)(47), even though conflicting results have been obtained regarding the role of SILT in anti-Salmonella responses (48).…”

Section: Discussionmentioning

confidence: 99%

Chemokine Receptor CXCR5 Supports Solitary Intestinal Lymphoid Tissue Formation, B Cell Homing, and Induction of Intestinal IgA Responses

Velaga

Herbrand

Friedrichsen

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

Solitary intestinal lymphoid tissue (SILT) comprises a spectrum of phenotypically diverse lymphoid aggregates interspersed throughout the small intestinal mucosa. Manifestations of SILT range from tiny lymphoid aggregates almost void of mature lymphocytes to large structures dominated by B cells. Large SILT phenotypically resemble a single Peyer’s patch follicle, suggesting that SILT might contribute to intestinal humoral immune responses. In this study, we track the fate of individual SILT in vivo over time and analyze SILT formation and function in chemokine receptor CXCR5-deficient mice. We show that, in analogy to Peyer’s patches, formation of SILT is invariantly determined during ontogeny and depends on CXCR5. Young CXCR5-deficient mice completely lack SILT, suggesting that CXCR5 is essential for SILT formation during regular postnatal development. However, microbiota and other external stimuli can induce the formation of aberrant SILT distinguished by impaired development of B cell follicles in CXCR5-deficient mice. Small intestinal transplantation and bone marrow transplantation reveal that defect follicle formation is due to impaired B cell homing. Moreover, oral immunization with cholera toxin or infection with noninvasive Salmonella fail to induce efficient humoral immune responses in CXCR5-deficient mice. Bone marrow transplantation of CXCR5-deficient recipients with wild-type bone marrow rescued B cell follicle formation in SILT but failed to restore full humoral immune responses. These results reveal an essential role of CXCR5 in Peyer’s patch and SILT development and function and indicate that SILT do not fully compensate for the lack of Peyer’s patches in T cell-dependent humoral immune responses.

show abstract

Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella

Cited by 9 publications

References 24 publications

Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis

Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis

Peyer's Patches Are Required for Intestinal Immunoglobulin A Responses toSalmonellaspp

Chemokine Receptor CXCR5 Supports Solitary Intestinal Lymphoid Tissue Formation, B Cell Homing, and Induction of Intestinal IgA Responses

Contact Info

Product

Resources

About