Isolated Nitella protoplasm is not excitable

Koppenhöfer, E; Ode, Andrea; Rimmel, Chr.; Schramm, M.; Schuback, P.; Schumann, Habil. Karl

doi:10.1016/0022-5193(77)90071-6

Cited by 3 publications

(1 citation statement)

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“…DISCUSSION Is There a Membrane? The presence of a 'true' biological membrane around the protoplasmic droplet has been questioned (18). But it has been shown that protease and lipase alter the biophysical properties of the protoplasmic droplets of Nitella (14) and that ionic channels are present in the membrane of protoplasmic droplets of C. corallina (13).…”

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confidence: 99%

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

Homblé¹

1987

Plant Physiol.

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The biophysical properties of voltage-dependent K+-channels of pro- (e.g. 8, 19, 26, 27), and that potassium ions are the main species transported in the leakage current (2). K+-channels also play a role in the light-induced membrane potential change in perfused Chara corallina cells (30) and in Eremosphaera viridis (17). Ion transpot through the plant cell membrane has also been studied by noise analysis of the membrane potential (6,(22)(23)(24). The minus two slope of the voltage noise power spectrum has been attributed to the activity of K+-channels (6). More recently the patch-clamp technique has been used to study the properties of a single K+-channel isolated from the plasmalemma of guard cells of Vicia faba (25) and of the membrane surrounding protoplasmic droplets of C. corallina cells (13).In the present work the time-and voltage-dependence of K+-channels were investigated on protoplasmic droplets by means of the tight-seal whole cell technique (9) which allows the intracellular ionic composition to be controlled. Two K+-channel activities with different time-and voltage-dependent characteristics were identified.

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Section: )mentioning

confidence: 99%

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

Homblé¹

1987

Plant Physiol.

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Cell Motility: I Cytoplasmic Movements

Dillon

1981

Ultrastructure, Macromolecules, and Evolution

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Generation of an Anatomically Based Geometric Coronary Model

Smith

Pullan

Hunter

2000

Annals of Biomedical Engineering

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A discrete anatomically accurate finite element model of the largest six generations of the coronary arterial network is developed. Using a previously developed anatomically accurate model of ventricular geometry the boundaries of the coronary mesh are defined from measured epicardial coronaries. Network topology is then generated stochastically from published anatomical data. Spatial information is added to this topological data using an avoidance algorithm accounting for global network geometry and optimal local branch angle properties. The generated vessel lengths, radii and connectivity are consistent with the published studies and a relativity even spatial distribution of vessels within the ventricular mesh is achieved. The local finite element coordinates of the coronary nodes within the ventricular mesh are calculated such that the coronary geometry can be recalculated within a deformed ventricular mesh.

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Isolated Nitella protoplasm is not excitable

Cited by 3 publications

References 10 publications

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

Cell Motility: I Cytoplasmic Movements

Generation of an Anatomically Based Geometric Coronary Model

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Isolated Nitella protoplasm is not excitable

Cited by 3 publications

References 10 publications

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K+-Channels of Protoplasmic Droplets of Chara corallina

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K+-Channels of Protoplasmic Droplets of Chara corallina

Cell Motility: I Cytoplasmic Movements

Generation of an Anatomically Based Geometric Coronary Model

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Product

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A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina

A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K⁺-Channels of Protoplasmic Droplets of Chara corallina