Isolates From Thymelaea Hirsuta Inhibit Progression Of Hepatocellular Carcinoma In Vitro And In Vivo

Badawy, Ahmed; Hassanean, Hashim A.; Ibrahim, Amany K.; Habib, Eman S.; El-Magd, Mohammed A.; Ahmed, Safwat A.

doi:10.1080/14786419.2019.1643859

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…This is crucial for cancer cells to maintain their uncontrollable proliferation. Therefore, triggering apoptosis is a main mode of action for most of the anti-cancer agents [16,17,19,[42][43][44][45]. In the present study, A. machrostachyum extract reduces EST size through induction of apoptosis as evidenced histologically (presence of apoptotic cells), and molecularly (up-regulation of the apoptotic genes p53, Bax, and caspase 3, and downregulation of the antiapoptotic gene Bcl2 as well as increase DNA damage).…”

Section: Discussionmentioning

confidence: 50%

“…The notable therapeutic outcomes of some medicinal plants and/or their phytochemicals lifted the bar for other synthetic drugs that could lead to severe adverse effects [9][10][11]. The cytotoxic effect of many medicinal plants against cancer cells is primarily owed to induction of apoptosis mainly through upregulation of the apoptotic genes (such as p53, Bax, caspase 3, 7, 8, and 9) and downregulation of the anti-apoptotic genes (Bcl2 and survivin) [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Sharawi

2020

BMC Complement Med Ther

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Background: The anti-cancer effect of the halophyte Arthrocnemum indicum, a member of Arthrocnemum family of salt-tolerant plants, was evaluated against colorectal cancer cell, CaCo2. However, the anti-cancer effect of another halophyte Arthrocnemum machrostachyum was not investigated yet. Herein, the anticancer effect of A. machrostachyum methanolic extract (AME) was evaluated against Ehrlich solid tumor (EST) in mice and the potential mechanism of action was also studied. Methods: Male Swiss albino mice (n = 28) were randomly divided into 4 groups (n = 7/group). Group 1 (negative control group); group 2 (EST) injected intramuscularly by 0.2 mL Ehrlich ascitic carcinoma (2 × 10 6 cells); and groups 3 and 4 injected intratumorally with AME (180 and 360 mg/kg body weight, respectively) at D12 trice weekly for 2 weeks. Gene expression, protein expression, DNA damage, and TNFa level in tumors were determined by real-time PCR, western blot, comet assay, and Elisa, respectively. Results: Treatment with AME induced anti-tumor effects against EST as indicated by 1) notable reduction in tumor size; 2) elevation in tissue necrosis and apoptosis, as confirmed histologically; 3) increased DNA fragmentation; 4) decreased expression of the apoptotic genes (p53, Bax and caspase 3), and increased expression of the antiapoptotic marker Bcl2; 5) significantly upregulated cell cycle regulatory genes Cdc2 and connexin26, and; 6) decreased TNFa levels in tumor tissues. Interestingly, a high dose of AME exhibited a more potent anti-tumor effect against EST. Conclusion: These findings indicate that AME has a potent antitumor effect against EST and could be used as an adjuvant to anticancer drugs to combat tumor, but after application of further confirmatory clinical trials.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Sharawi

2020

BMC Complement Med Ther

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“…This result was in line with the finding of Kakengi et al, (2003); Olugbemi and Banjo (2012) who found that the inclusion of M. oleifera meal in the diet of the broilers significantly enhanced their weight gain at 1% level which was significantly higher than the control. Several research findings reported that herbal extracts could increase broiler performance by improving live weight gain (Al-Kassie and Jameel, 2009;Badawy et al, 2019;Jamroz et al, 2003).…”

Section: Body Weight Gainmentioning

confidence: 99%

Effect of some herbal extracts on growth performance and liver enzymes in broilers

Fathallah

Shawky

Gaffer

et al. 2020

Kafrelsheikh Veterinary Medical Journal

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Objective: After the ban in 2006 of the use of antibiotic growth promoters, the search for an alternative led to the utilization of plants like Moringa oleifera Lam. The present study was carried out to determine the effect of Moringa and Echinacea supplementation on drinking water on growth performance and liver function. Methods: One hundred and fifty one-day-old chicks (Cobb strain) were randomly allotted to three experimental groups G1 (basic diet, control group), G2 (basic diet + watering of Echinacea herbal extract), and G3 (basic diet + watering of Moringa herbal extract). The liver function test was performed through the detection of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in plasma. Results: The herbal mixture groups (G2 and G3) significantly (P<0.05) improved the growth performance while no significant difference in serum levels of liver function enzymes AST and ALT as compared to the control group. Conclusion: With these results, we could conclude that replacing antibiotic growth promoters with herbal supplements is safe and improve the growth performance of broilers.

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“…Natural products derived from plants have served as an important source of pharmacologic agents for several diseases [ 2 ], including cancer [ 3 ]. An example is the Thymelaeaceae family of plants, that have been shown to exhibit many pharmacological activities [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. For example, extracts of Daphne giraldii [ 4 ] and Wikstroemia indica [ 5 ] from the Thymelaeaceae family of plants are known to contain various pharmacologically active constituents, including: flavonoids [ 6 ], coumarins [ 7 ], diterpenoids [ 8 ], lignans, volatile oils and polysaccharides [ 9 ] that have been used for the tretament of several ailments.…”

Section: Introductionmentioning

confidence: 99%

“…T. hirsute contains flavonoids, coumarins, tannins, and saponins [ 13 ], and has been demonstarted to have antiviral activity against HIV-1 [ 14 ], antioxidant activity [ 15 ], antidiabetic and antihypertensive activities [ 16 ]. Other studies have also shown isolates from T. hirsuta to have significant cytotoxicity against hepatocellular carcinoma (HCC) [ 17 ], which is one of the leading causes of cancer deaths worldwide, with a rapidly increasing incidence rate in Egypt [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

New Antiproliferative Triflavanone from Thymelaea hirsuta—Isolation, Structure Elucidation and Molecular Docking Studies

et al. 2021

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In this study isolates from Thymelaea hirsuta, a wild plant from the Sinai Peninsula of Egypt, were identified and their selective cytotoxicity levels were evaluated. Phytochemical examination of the ethyl acetate (EtOAc) fraction of the methanolic (MeOH) extract of the plant led to the isolation of a new triflavanone compound (1), in addition to the isolation of nine previously reported compounds. These included five dicoumarinyl ethers found in Thymelaea: daphnoretin methyl ether (2), rutamontine (3), neodaphnoretin (4), acetyldaphnoretin (5), and edgeworthin (6); two flavonoids: genkwanin (7) and trans-tiliroside (8); p-hydroxy benzoic acid (9) and β sitosterol glucoside (10). Eight of the isolated compounds were tested for in vitro cytotoxicity against Vero and HepG2 cell lines using a sulforhodamine-B (SRB) assay. Compounds 1, 2 and 5 exhibited remarkable cytotoxic activities against HepG2 cells, with IC50 values of 8.6, 12.3 and 9.4 μM, respectively, yet these compounds exhibited non-toxic activities against the Vero cells. Additionally, compound 1 further exhibited promising cytotoxic activity against both MCF-7 and HCT-116 cells, with IC50 values of 4.26 and 9.6 μM, respectively. Compound 1 significantly stimulated apoptotic breast cancer cell death, resulting in a 14.97-fold increase and arresting 40.57% of the cell population at the Pre-G1 stage of the cell cycle. Finally, its apoptosis-inducing activity was further validated through activation of BAX and caspase-9, and inhibition of BCL2 levels. In silico molecular docking experiments revealed a good binding mode profile of the isolates towards Ras activation/pathway mitogen-activated protein kinase (Ras/MAPK); a common molecular pathway in the development and progression of liver tumors.

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Isolates From Thymelaea Hirsuta Inhibit Progression Of Hepatocellular Carcinoma In Vitro And In Vivo

Cited by 18 publications

References 24 publications

Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Effect of some herbal extracts on growth performance and liver enzymes in broilers

New Antiproliferative Triflavanone from Thymelaea hirsuta—Isolation, Structure Elucidation and Molecular Docking Studies

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