Isolates of the Nematophagous Fungus Pochonia chlamydosporia Are Endophytic in Banana Roots and Promote Plant Growth

Mingot-Ureta, Cristina; López-Moya, Federico; López-Llorca, Luis Vicente

doi:10.3390/agronomy10091299

Cited by 18 publications

(20 citation statements)

References 45 publications

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“…Waweru et al [33] The culture filtrate of BF7 significantly increased growth parameters viz., shoot length, root length and pseudostem girth by 20.39, 22.06 and 22.73 %, respectively followed by BF28 isolate which was recorded by 17.69, 18.89 and 19.05%, respectively in banana under pot culture conditions. The present results were also in conformity with the earlier reports of Mingot-Ureta et al [38] found that the endophytic fungal strains significantly increased the growth parameters viz., pseudostem height, girth and number of leaves in banana plants under glasshouse conditions.…”

Section: Pot Culture Evaluation Of Fungal Endophytes Against M Incognitasupporting

confidence: 94%

Endophytic Fungi as Potential Bio-Control Agents against Root Knot Nematode, Meloidogyne incognita in Banana

Ganeshan

Vetrivelkalai

Bhagawati

et al. 2021

CJAST

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A Survey was conducted in 12 districts of Assam to collect 92 healthy banana root samples. A total of 55 fungal isolates were successfully isolated from commercial banana cultivars. The culture filtrates were extracted from 55 endophytic fungal isolates and screened against root knot nematode, Meloidogyne incognita in vitro and pot culture studies. Among them, five fungal isolates viz., EF4, BF7, BF27, BF28 and BF35 showed 100% inhibition of egg hatching and 96.33 to 81.33% juvenile mortality of M. incognita with an exposure period of 72h when compared to other isolates and control. On paddy seed treatment with endophytic fungi of five promising isolates, two isolates viz., BF7 and BF28 significantly enhanced germination percentage (82.67%, 73.33%) and vigour index (62.91, 47.24%), respectively. The selected five endophytic fungal isolates were evaluated for their efficacy against M. incognita in banana under pot culture conditions. The study revealed that culture filtrates of BF7 and BF28 significantly reduced the soil and root nematode population, number of adult females, egg masses and root gall index of M.incognita compared to untreated control. The isolates BF7 and BF28 also significantly increased the growth parameters viz., pseudostem height, root length and pseudostem girth. These promising endophytic fungal isolates viz., BF7 and BF28 were identified as non-pathogenic Fusarium oxysporum strains (Accession no. MN567668) and (Accession no. MN567710), respectively by PCR -18S rRNA of ITS region of gene sequence and phylogenetic tree construction.

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Section: Pot Culture Evaluation Of Fungal Endophytes Against M Incognitasupporting

confidence: 94%

Endophytic Fungi as Potential Bio-Control Agents against Root Knot Nematode, Meloidogyne incognita in Banana

Ganeshan

Vetrivelkalai

Bhagawati

et al. 2021

CJAST

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“…However, the mechanism activated to prime the whole plant for enhanced defense is only partially elucidated. Previous studies reported that P. chlamydosporia colonization may underpin a defense response in roots [9][10][11]. The assays carried out with different pathogens and plant hosts confirmed the capacity of P. chlamydosporia to promote a systemically induced response before or after the insurgence of a biotic stress factor.…”

Section: Discussionsupporting

confidence: 64%

“…Roots colonized by P. chlamydosporia differentially modulated the expression of key genes involved in plant defense responses in leaves of both monocot and dicot hosts. Moreover, the endophyte is known to colonize roots without inducing significant damage [10,20]. The genes herein examined (PIN II, PAL, LOX, and PR1) are involved in defense pathways by encoding pathogenesis-related proteins or JA-or SA-signaling molecules.…”

Section: Discussionmentioning

confidence: 99%

“…Endophyte colonization can activate defense responses at the molecular level, upon additional biotic or abiotic stresses including root-associated microorganisms or pathogens, with an increase of the defense-related gene expression [6,7]. In the case of P. chlamydosporia, roots of monocot and dicot host plants showed that the endophyte colonization induced the expression of several defense genes in roots, eliciting an early host defense response, which is effective in the presence of pests such as endoparasitic nematodes [8][9][10][11]. Endophytic P. chlamydosporia induced changes in the transcriptome of tomato roots, highlighting a specific modulation of stress-responsive transcripts related to a selective activation of defense pathways [11].…”

Section: Introductionmentioning

confidence: 99%

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Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions

Tolba

Rosso

Pentimone

et al. 2021

Plants

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A study was carried out on the effect of the root endophytic fungus Pochonia chlamydosporia on plant systemic signal of defense related genes during fungal or nematode parasitism. Different biotic stress factors were examined, inoculating roots of dicot and monocot hosts with the endophyte, and measuring the expression of defense genes in leaves. A first greenhouse assay was carried out on expression of PAL, PIN II, PR1 and LOX D in leaves of tomato cv Tondino inoculated with Phytophthora infestans (CBS 120920), inoculating or not the roots of infected plants with P. chlamydosporia DSM 26985. In a second assay, plants of banana (Musa acuminata cv Grand Naine) were artificially infected with Fusarium oxysporum f. sp. cubense Tropical race 4 (TR4) and inoculated or not with DSM 26985. In a further experiment, banana plants were inoculated or not with P. chlamydosporia plus juveniles of the root knot nematode (RKN) Meloidogyne incognita. A similar assay was also carried out in vitro with adults and juveniles of the lesion nematode Pratylenchus goodeyi. Differential expression of the defense genes examined was observed for all plant-stress associations, indicative of early, upward systemic signals induced by the endophyte. Changes in expression profiles included a 5-fold down-regulation of PIN II at 2 dai in leaves of tomato plants treated with P. infestans and/or P. chlamydosporia, and the up-regulation of PAL by the endophyte alone, at 2 and 7 dai. In the TR4 assay, PR1 was significantly up-regulated at 7 dai in banana leaves, but only in the P. chlamydosporia treated plants. At 10 dai, PIN II expression was significantly higher in leaves of plants inoculated only with TR4. The banana-RKN assay showed a PR1 expression significantly higher than controls at 4 and 7 dai in plants inoculated with P. chlamydosporia alone, and a down-regulation at 4 dai in leaves of plants also inoculated with RKN, with a PR1 differential up-regulation at 10 dai. Pratylenchus goodeyi down-regulated PIN at 21 dai, with or without the endophyte, as well as PAL but only in presence of P. chlamydosporia. When inoculated alone, the endophyte up-regulated PR1 and LOX. The gene expression patterns observed in leaves suggest specific and time-dependent relationships linking host plants and P. chlamydosporia in presence of biotic stress factors, functional to a systemic, although complex, activation of defense genes.

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“…This could mean that Pc is secreting metabolites homologous to plant hormones in order to facilitate plant root colonization. This could be one of the reasons why Pc increases banana plant growth and development (Mingot‐Ureta et al ., 2020 ). Finally, endophytism in Pc modifies the expression of transcription factors.…”

Section: Discussionmentioning

confidence: 99%

Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism

Suarez-Fernandez

Sambles

López-Moya

et al. 2021

Environmental Microbiology

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Climate change makes plant-parasitic nematodes (PPN) an increasing threat to commercial crops. PPN can be managed sustainably by the biocontrol fungus Pochonia chlamydosporia (Pc). Chitosan generated from chitin deacetylation enhances PPN parasitism by Pc. In this work, we investigate the molecular mechanisms of Pc for chitosan resistance and root-knot nematode (RKN) parasitism, using transcriptomics. Chitosan and RKN modify the expression of Pc genes, mainly those involved in oxidation-reduction processes. Both agents significantly modify the expression of genes associated to 113 GO terms and 180 Pc genes. Genes encoding putative glycoproteins (Pc adhesives) to nematode eggshell, as well as genes involved in redox, carbohydrate and lipid metabolism trigger the response to chitosan. We identify genes expressed in both the parasitic and endophytic phases of the Pc lifecycle; these include proteases, chitosanases and transcription factors. Using the Pathogen-Host Interaction database (PHI-base), our previous RNA-seq data and RT-PCR of Pc colonizing banana we have investigated genes expressed both in the parasitic and endophytic phases of Pc lifecycle.

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Isolates of the Nematophagous Fungus Pochonia chlamydosporia Are Endophytic in Banana Roots and Promote Plant Growth

Cited by 18 publications

References 45 publications

Endophytic Fungi as Potential Bio-Control Agents against Root Knot Nematode, Meloidogyne incognita in Banana

Endophytic Fungi as Potential Bio-Control Agents against Root Knot Nematode, Meloidogyne incognita in Banana

Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions

Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism

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