Isolation and antimicrobial activities of a novel discolornolide and other compounds from Monanthotaxis discolor

Abstract

Characterisation of a novel discolornolide (1) isolated from a first time investigation of Monanthotaxis discolor is described. Other 6 known compounds, karatavin (2), Nacetylanonaine (3), quercetin-3-O--arabinose (4), stigmasterol (5 ), a mixture of stigmasterol and -sitosterol (6) and octahydro-5-isopropyl-3-methyl-2-methyleneazulene-8,8-diol (7) isolated are also reported. The structures were established by spectroscopic methods.Citotoxicities and antimicrobial activities of the compounds and crude extracts are also reported whereby compound 1 showed in vitro antifungal activity against Candida albicans and Aspergilus niger at concentrations of 0.13 and 0.17 mg/ml with zones of inhibitions of 7.0 and 5.5 mm respectively. The compound also showed cyitotoxic activity in the brine shrimp test with LC 50 of 5.88 g/ml. Also compound 4 exhibited antibacterial activity against E. coli and S.aureus. The compound also exhibited cytotoxic activities in the brine shrimp test with LC 50 of 24.73 g/mL. The crude extracts exhibited varying citotoxic and in vitro antimicrobial activities.

In vitro and in silico cytotoxicity effects of Zanthoxylum simulans Hance. fruit bark extract against gastric cancer cell lines

“…N-acetylanonaine is an alkaloid, found in many plants such as Zanthoxylum simulans, and Magnolia kachirachirai [22,23]. This compound has been reported for its complete inhibitory ability on platelet aggregation induced by arachidonic acid and collagen as well as antimicrobial activity in both bacteria and fungi such as C. albicans and S. aureus [23,24]. In our study, N-acetylanonaine exhibited the ability to inhibit both AKT1 and MAPK1 targets with docking scores of -9.8 and -9.1 kcal/mol, respectively.…”