Isolation and Antimicrobial Susceptibility Profile of Escherichia coli O157 : H7 from Raw Milk of Dairy Cattle in Holeta District, Central Ethiopia

Ababu, Ashenafi; Endashaw, Dereje; Fesseha, Haben

doi:10.1155/2020/6626488

Cited by 35 publications

(49 citation statements)

References 31 publications

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“…Owing to its important ingredients, it is widely accepted as a full diet. 2,3 In Ethiopia, however, milk consumed annually is poor in comparison with other developing countries' total dairy intake. Local milk production fails to meet the milk requirements of the country because of low inputs and common cattle health conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Bovine mastitis leads to a decrease in milk production, increases culling rate, incurs medical costs, and often leads to severe deaths from infections. 3,6,10 Mastitis is of extreme zoonotic importance since the milk is unsafe for human consumption. This is due to the risk of bacterial contamination of the milk from infected cows.…”

Section: Introductionmentioning

confidence: 99%

“…This is due to the risk of bacterial contamination of the milk from infected cows. 3,11,12 Clinical and subclinical mastitis (SCM) are the two types of mastitis. Variations in physical characteristics of milk, udder enlargement, redness, and increase in udder temperature were commonly observed as a clinical manifestation of clinical mastitis, whilst SCM infected dairy cattle display no significant milk or udder changes and can only be detected by somatic cell count (SCC) or bacterial culture.…”

Section: Introductionmentioning

confidence: 99%

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Study on Prevalence of Bovine Mastitis and Associated Risk Factors in Dairy Farms of Modjo Town and Suburbs, Central Oromia, Ethiopia

Fesseha¹,

Mathewos²,

Aliye³

et al. 2021

VMRR

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Background:In the global dairy industry, mastitis is the main economic significant disease of cattle. Milk and other dairy outputs are scarce in developed countries, including Ethiopia. Methods: In this cross-sectional investigation in the Modjo district, milk samples were collected aseptically from 384 randomly selected lactating cattle to investigate the prevalence of clinical and sub-clinical mastitis and determine the possible risk factors and isolate bacterial pathogens causing mastitis. Besides, clinical mastitis cases have been reported by veterinarians based on milk, udder, or systemic cow anomalies, whereas the presence of subclinical mastitis was determined using California Mastitis Test (CMT). Results:The research revealed that subclinical mastitis (71.02%) is more prevalent in dairy farms of the study area than in the clinical type (28.9%). The quarter-level frequency was 36.9%; from which, 34.9% and 3.4% were from subclinical form and blind teat, respectively. There was a significant correlation between the frequency of mastitis in lactating cows (p < 0.05) and factors, such as breed, age, body condition score, herd size, milking mastitic cow at the end, and previous mastitis history. The dominant mastitis-causing agents isolated in this study were Staphylococcus aureus (40.3%), Streptococcus species (24.3%), Coagulasenegative Staphylococcus (12.5%), E. coli (8.3%), Staphylococcus hyicus (3.5%), and Staphylococcus intermedius (1.4%). The high occurrence of mastitis, particularly sub-clinical mastitis, revealed significant economic potential losses in dairy farms in the research district. Conclusion: Therefore, appropriate measures aimed at increasing the understanding and hygiene milking methods of dairy farmers, routine monitoring for subclinical mastitis, dry cow therapy, and culling of chronically contaminated cows to reduce bovine mastitis and its impact on milk production and food security.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study on Prevalence of Bovine Mastitis and Associated Risk Factors in Dairy Farms of Modjo Town and Suburbs, Central Oromia, Ethiopia

Fesseha¹,

Mathewos²,

Aliye³

et al. 2021

VMRR

Self Cite

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“…In Ethiopia, a country which ranks second after Nigeria in the health burden of zoonotic diseases in Africa [28], the epidemiology of food-borne pathogens especially that of pathogenic E. coli is not well studied. However, recently, there is an increasing trend of reporting the occurrence level of the organism in beef, dairy products, and fish [2,3,9,16,[29][30][31][32][33][34][35][36][37]. Besides this, current and detailed studies on the prevalence of antibiotic resistance and multidrug phenotypes of enterohemorrhagic E. coli O157 : H7 in foods of animal origin in different catering establishments are very limited in Ethiopia except for the report of [2,3,9] on beef at butcher shops and restaurants and raw milk and dairy products such as yogurt and cheese derived from raw milk at cafeterias, restaurants, open markets, and supermarkets in Central Ethiopia.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli O157 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

Geresu

Regassa

2021

The Scientific World Journal

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Escherichia coli O157 : H7 (E. coli O157 : H7) has been found to be the major cause of food-borne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial-resistant strains. Hitherto, little is known about the carriage of E. coli O157 : H7 and its antimicrobial susceptibility profile in the food of animal origin in Ethiopia. This study aimed to determine the occurrence and multidrug resistance profile of E. coli O157 : H7 from food of animal origin at different catering establishments in the selected study settings of Arsi Zone. One hundred ninety-two animal origin food items, namely, raw/minced meat (locally known as “Kitfo,” “Kurt,” and “Dulet”), raw milk, egg sandwich, and cream cake samples were collected and processed for microbiological detection of E. coli O157 : H7. Out of 192 samples, 2.1% (4/192) were positive for E. coli O157 : H7. Two E. coli O157 : H7 isolates were obtained from “Dulet” (6.3%) followed by “Kurt” (3.1%, 1/32) and raw milk (3.1%, 1/32), whereas no isolate was obtained from “Kitfo,” egg sandwich, and cream cake samples. Of the 4 E. coli O157 : H7 isolates subjected to 10 panels of antimicrobial discs, 3 (75%) were highly resistant to kanamycin, streptomycin, and nitrofurantoin. Besides, all the isolates displayed multidrug resistance phenotypes, 3 to 5 antimicrobial resistance, amid kanamycin, streptomycin, nitrofurantoin, tetracycline, and chloramphenicol. The occurrence of multidrug-resistant E. coli O157 : H7 isolates from foods of animal origin sampled from different catering establishments reveals that the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices did not comply with the recommended standards. Thus, this finding calls for urgent attention toward appropriate controls and good hygienic practices in different catering establishments dealing with consuming raw/undercooked foods of animal origin.

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“…The closest results to this study for the resistant strains of several human isolates were reported by Momtaz et al [ 66 ], who determined that the isolates were more resistant to Oxytetracycline 86%, and Ranjbar et al [ 67 ], Gentamycin, Ciprofloxacin, and aminoglycosides. Higher results for antibiotic resistance were observed by Ababu et al [ 68 ], who noted that the resistance of the isolates for both Oxytetracycline and Gentamycin was 100%. On the other hand, Al-Ajmi et al [ 43 ] stated that 100% of STEC isolates were susceptible to Ciprofloxacin and 84% for Amoxicillin.…”

Section: Discussionmentioning

confidence: 69%

Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt

et al. 2021

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This study aims to determine the prevalence of STEC in she-camels suffering from mastitis in semi-arid regions by using traditional culture methods and then confirming it with Serological and molecular techniques in milk samples, camel feces, as well as human stool samples for human contacts. In addition, an antibiotic susceptibility profile for these isolates was investigation. Mastitic milk samples were taken after California Mastitis Test (CMT) procedure, and fecal samples were taken from she-camels and human stool samples, then cultured using traditional methods to isolate Escherichiacoli. These isolates were initially classified serologically, then an mPCR (Multiplex PCR) was used to determine virulence genes. Finally, both camel and human isolates were tested for antibiotic susceptibility. Out of a total of 180 she-camels, 34 (18.9%) were mastitic (8.3% clinical and 10.6% sub-clinical mastitis), where it was higher in camels bred with other animals. The total presence of E. coli was 21.9, 13.9, and 33.7% in milk, camel feces, and human stool, respectively, whereas the occurrence of STEC from the total E. coli isolates were 36, 16, and 31.4% for milk, camel feces, and stool, respectively. Among the camel isolates, stx1 was the most frequently detected virulence gene, while hlyA was not detected. The most detected virulence gene in human isolates was stx2 (45.5%), followed by stx1. Camel STEC showed resistance to Oxytetracycline only, while human STEC showed multiple drug resistance to Amoxicillin, Gentamycin, and Clindamycin with 81.8, 72.7, and 63.6%, respectively. Breeding camels in semi-arid areas separately from other animals may reduce the risk of infection with some bacteria, including E. coli; in contrast, mixed breeding with other animals contributes a significant risk factor for STEC emergence in camels.

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Isolation and Antimicrobial Susceptibility Profile of Escherichia coli O157 : H7 from Raw Milk of Dairy Cattle in Holeta District, Central Ethiopia

Cited by 35 publications

References 31 publications

Study on Prevalence of Bovine Mastitis and Associated Risk Factors in Dairy Farms of Modjo Town and Suburbs, Central Oromia, Ethiopia

Study on Prevalence of Bovine Mastitis and Associated Risk Factors in Dairy Farms of Modjo Town and Suburbs, Central Oromia, Ethiopia

Escherichia coli O157 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt

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