Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh

Abstract

An isolation and identification of phenolic compounds from Halban Leaves (Vitex pinnata Linn), a traditional medicinal plant in Aceh, has been done. Halban leaves were extracted through methanol maceration followed by isolation. The methanol extract went into n-hexane and ethyl acetate partition consecutively resulting in 3 different fractions of ethyl acetate and n-hexane extract. The ethyl acetate fraction demonstrated the most promising antioxidant and cytotoxic activity. Therefore, purification of ethyl acetate fraction was done by column chromatography [SiO2; (i) n-hexane-ethyl acetate 10:1, 1:1 ; (ii) n-hexane-ethyl acetate = 2 : 1); (iii) n-hexane-acetone = 1:1]. The pure isolate as identified by using UV, IR, NMR, and MS spectra afforded 2 phenolic compounds, namely (1) 4-hydroxymethyl benzoate and (2) p-hydroxymethyl benzoic acid (PHBA). The antioxidant activity of compound 1 and cytotoxicity activity of 2 expressed in IC50 and LD50 was 41.08 ppm and 59.41 ppm, respectively.Abstrak: Isolasi dan identifikasi senyawa fenolik dari Daun Halban (Vite xpinnata Linn) tanaman obat tradisional asal Aceh telah dilakukan. Isolasi ini dilakukan dengan cara mengekstraksi daun halban dengan cara maserasi menggunakan pelarut metanol, Ekstrak metanol kemudian dipartiisi dengan n-heksan dan etilasetat secara berturut-turut sehingga diperoleh tiga ekstrak yaitu ekstrak etil asetat, n-heksan dan air. Ekstrak etil asetat memiliki bioaktivitas sebagai antioksidan dan juga toksisitas. Pemurnian dilanjutkan dengan cara kromatografi kolom [SiO2; (i) n-heksan-etilasetat 10:1, 1:1 ; (ii) n-heksan-etilasetat = 2 : 1); (iii) n-heksan-aseton = 1:1] dan diperoleh isolat murni. Isolat murni tersebut diidentifikasi berdasarkan interpretasi data spektra UV, IR, NMR dan MS. Hasil interpretasi data menunjukkan daun halban (V. pinnata Linn) mengandung dua senyawa fenolik yaitu (1) 4-hidroksi metil benzoat dan (2) asam para hidroksi benzoat (PHBA). Senyawa 1 memiliki aktivitas antioksidan dengan nilai IC50 = 41,08bpj dan senyawa 2 memiliki aktivitas toksisitas dengan nilai LC50 = 59,41.

Psychoreligious Dimensions on Therapy Perspective in the Middle of the Covid-19 Pandemic

“…When discussing the cognitive-religious aspects in the perspective of Islam, the therapist must be able to show the rationality of each Islamic teaching to the client (Faraz et al, 2022). The practice of therapy in a time of pandemic which is full of chaos, be it social, political, or even in scientific narratives, because many of the opposing parties are medical professionals, making the practice of therapy is increasingly difficult to carry out, and therapists face more and more obstacles in their work in dealing with their clients (Mastura et al, 2020).…”

Antioxidant Activity-Guided Isolation of Phenolic Compounds from Leaves of Vitex pinnata (Lamiaceae)

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Identification of acetogenin compound, sabadelin from soursop seeds, Annona muricata (Annonaceae)

“…As a continuation of previous research in the search for bioactive compounds from Indonesian medicinal plants, such as quercitrin from the leaves of Dendrophthoe pentandra (L.) Mig as antioxidant and antibacterial (Hadiyanti R. et al, 2019;Sembiring H. et al, 2022);anticancer from rodent tuber, Typhonium flagelliforme (Sianipar NF. et al, 2020); phenolic compounds from halban leaves, Vitex pinnata Linn as an antioxidant (Mastura et al, 2020); n-hexane extract of Myrica fragrans as an antioxidant and antitumor activities against MCF-7 cell line (Ginting B.et al, 2020); stilbinoids compound from the bark of Raru, Vatica pauciflora Baume has cytotoxicity (Kartika R. et al, 2021); and the neem plant, Azadirachta indica A. Juss has bioinsecticide activities (Prianto AH. et al, 2022).…”